<i>Brassica napus</i><scp>GLABRA</scp>3‐1 promotes anthocyanin biosynthesis and trichome formation in true leaves when expressed in <i>Arabidopsis thaliana</i>

Gao, Chenhao; Guo, Yuan; Wang, Jianjun; Li, Dong; Liu, Kaige; Qi, Shihua; Jin, Changyu; Duan, Shengwen; Gong, Jingyun; Li, Zixiong; Chen, Mingxun

doi:10.1111/plb.12633

Cited by 13 publications

(6 citation statements)

References 56 publications

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“…However, MIXTA could not rescue the trichome phenotype of gl1 mutant of Arabidopsis (Payne et al , 1999). GL3 , a bHLH transcription factor, is another key gene of the MYB/bHLH/WD complex (Morohashi et al ., 2009; Gao et al ., 2018). However, the expression of SlGL3 driven by AtGL3 promoter could not promote the trichome formation in Arabidopsis (Tominaga‐Wada et al ., 2013).…”

Section: Discussionmentioning

confidence: 99%

HOMEODOMAIN PROTEIN8 mediates jasmonate‐triggered trichome elongation in tomato

Hua

Chang

et al. 2021

New Phytologist

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Plant hormones can adjust the physiology and development of plants to enhance their adaptation to biotic and abiotic challenges. Jasmonic acid (JA), one of the immunity hormones in plants, triggers genome-wide transcriptional changes in response to insect attack and wounding. Although JA is known to affect the development of trichomes, epidermal appendages that form a protective barrier against various stresses, it remains unclear how JA interacts with developmental programs that regulate trichome development. In this study, we show that JA affects trichome length in tomato by releasing the transcriptional repression mediated by Jasmonate ZIM (JAZ) proteins. We identified SlJAZ4, a negative regulator preferentially expressed in trichomes, as the critical component in JA signaling in tomato trichomes. We also identified a homeodomain-leucine zipper gene, SlHD8, as the downstream regulator of JA signaling that promotes trichome elongation. SlHD8 is also highly expressed in trichomes and physically interacts with SlJAZ4. Loss-of-function mutations in SlHD8 caused shorter trichomes, a phenotype that was only partially rescued by methyl jasmonate treatment. Our dual-luciferase and chromatin immunoprecipitation-quantitative PCR assays revealed that SlHD8 regulates trichome elongation by directly binding to the promoters of a set of cellwall-loosening protein genes and activating their transcription. Together, our findings define SlHD8-SlJAZ4 as a key module mediating JA-induced trichome elongation in tomato.

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Section: Discussionmentioning

confidence: 99%

HOMEODOMAIN PROTEIN8 mediates jasmonate‐triggered trichome elongation in tomato

Hua

Chang

et al. 2021

New Phytologist

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“…The expression level of Fudingdabai haplotype B of W03g008694 was significantly higher than that of Fudingdabai haplotype A, which was also indicated by scaled‐read counts for each phased SNP (adjusted P < 0.05; anova ; Figure 4(b)). Another gene ( W11g023219 ) encodes a bHLH transcription factor and the function of its homolog in regulating anthocyanin biosynthesis has been demonstrated in many species (Feyissa et al ., 2009; Tominaga‐Wada et al ., 2013; Gao et al ., 2018; Su et al ., 2020). Ten and 11 accessions harbored Fudingdabai haplotype A and haplotype B of W11g023219 , respectively, and haplotype B displayed markedly higher expression than haplotype A (Figure 4(a)).…”

Section: Resultsmentioning

confidence: 99%

A phased genome based on single sperm sequencing reveals crossover pattern and complex relatedness in tea plants

et al. 2020

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SUMMARYFor diploid organisms that are highly heterozygous, a phased haploid genome can greatly aid in functional genomic, population genetic and breeding studies. Based on the genome sequencing of 135 single sperm cells of the elite tea cultivar ‘Fudingdabai’, we herein phased the genome of Camellia sinensis, one of the most popular beverage crops worldwide. High‐resolution genetic and recombination maps of Fudingdabai were constructed, which revealed that crossover (CO) positions were frequently located in the 5′ and 3′ ends of annotated genes, while CO distributions across the genome were random. The low CO frequency in tea can be explained by strong CO interference, and CO simulation revealed the proportion of interference insensitive CO ranged from 5.2% to 11.7%. We furthermore developed a method to infer the relatedness between tea accessions and detected complex kinship and genetic signatures of 106 tea accessions. Among them, 59 accessions were closely related with Fudingdabai and 31 of them were first‐degree relatives. We additionally identified genes displaying allele specific expression patterns between the two haplotypes of Fudingdabai and genes displaying significantly differential expression levels between Fudingdabai and other haplotypes. These results lay the foundation for further investigation of genetic and epigenetic factors underpinning the regulation of gene expression and provide insights into the evolution of tea plants as well as a valuable genetic resource for future breeding efforts.

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“…Unfortunately, all bHLH genes in L. bicolor have low homology (<30%) with AtGL3 [ 28 ], except for a key gene for salt gland development called LbbHLH (Lb1G04899, homology of 30–40%) [ 29 ]. LbbHLH can interact with LbTTG1 to inhibit the production of salt glands in the epidermal structure of L. bicolor [ 28 ], which was different from the role of AtGL3 in Arabidopsis in initiating trichome formation [ 30–32 ]. Thus, the roles of bHLH family genes need to be investigated in more detail, which is relevant to the study of salt glands.…”

Section: Discussionmentioning

confidence: 99%

Genome-wide identification of bHLH transcription factors and functional analysis in salt gland development of the recretohalophyte sea lavender (Limonium bicolor)

Wang,

Yuan

2024

Horticulture Research

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Transcription factors with basic helix–loop–helix (bHLH) structures regulate plant growth, epidermal structure development, metabolic processes, and responses to stress extensively. Sea lavender (Limonium bicolor) is a recretohalophyte with unique salt glands in the epidermis that make it highly resistant to salt stress, and contribute to improve saline lands. However, the features of the bHLH transcription factor family in L. bicolor are largely unknown. Here, we systematically analyzed the characteristics, localization, and phylogenetic relationships of 187 identified bHLH family genes throughout the L. bicolor genome, as well as their cis-regulatory promoter elements, expression patterns, and key roles in salt gland development or salt tolerance by genetic analysis. Nine verified L. bicolor bHLH genes are expressed and the encoded proteins function in the nucleus, among which the proteins encoded by Lb2G14060 and Lb1G07934 also localize to salt glands. Analysis of CRISPR-Cas9-generated knockout mutants and overexpression lines indicated that the protein encoded by Lb1G07934 is involved in the formation of salt glands, salt secretion and salt resistance, indicating that bHLH genes strongly influence epidermal structure development and stress responses. The current study lays the foundation for further investigation of the effects and functional mechanisms of bHLH genes in L. bicolor and paves the way for selecting salt-tolerance genes that will enhance salt resistance in crops and for the improvement of saline soils.

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Brassica napusGLABRA3‐1 promotes anthocyanin biosynthesis and trichome formation in true leaves when expressed in Arabidopsis thaliana

Cited by 13 publications

References 56 publications

HOMEODOMAIN PROTEIN8 mediates jasmonate‐triggered trichome elongation in tomato

HOMEODOMAIN PROTEIN8 mediates jasmonate‐triggered trichome elongation in tomato

A phased genome based on single sperm sequencing reveals crossover pattern and complex relatedness in tea plants

Genome-wide identification of bHLH transcription factors and functional analysis in salt gland development of the recretohalophyte sea lavender (Limonium bicolor)

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