<i>Borrelia tanukii</i> Sp. Nov. and <i>Borrelia turdae</i> Sp. Nov. Found from Ixodid Ticks in Japan: Rapid Species Identification by 16S rRNA Gene‐Targeted PCR Analysis

Fukunaga, Masao; Hamase, Akiko; Okada, Keiji; Nakao, Minoru

doi:10.1111/j.1348-0421.1996.tb01154.x

Cited by 116 publications

(61 citation statements)

References 25 publications

(30 reference statements)

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“…Groups Hk501 and Ya501 are restricted to Japan (13,27). Recently, these two genomic groups were reclassified as the new species Borrelia tanuki and Borrelia turdae, respectively (15). Groups VS116 and PotiB2 comprise European isolates, whereas group DN127 includes only North American isolates (31).…”

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confidence: 99%

Genetic and Phenotypic Analysis of Borrelia valaisiana sp. nov. (Borrelia Genomic Groups VS116 and M19)

Wang¹,

Dam²,

Flèche

et al. 1997

International Journal of Systematic Bacteriology

192

131

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To clarify the taxonomic status of two recently described Borrelia genomic groups, groups VS116 and M19, three group VS116 strains and eight group M19 strains isolated from Ixodes ricinus ticks in Switzerland, The Netherlands, and the United Kingdom were characterized. PCR-restriction fragment length polymorphism (RFLP) analysis of the 5s-23s intergenic spacer amplicon, rRNA gene restriction analysis, 16s rRNA gene sequence analysis, randomly amplified polymorphic DNA (RAPD) fingerprinting, sodium dodecyl sulfatepolyacrylamide gel electrophoresis (SDS-PAGE), and immunoblotting with monoclonal antibodies were used for genetic and phenotypic analysis. The PCR-RFLP and RAPD patterns of three group VS116 strains and eight group M19 strains were identical but differed from those of Borrelia burgdorferi sensu stricto, Borrelia garinii, Borrelia afielii, and Borrelia japonica. DNAs from all group VS116 and M19 strains yielded three fragments (6.9, 3.2, and 1.4 kb) and four fragments (2.1, 1.2, 0.8, and 0.6 kb) after digestion with EcoRV and HindIII, respectively, hybridizing with an Escherichia coli 16s +23S cDNA probe. The SDS-PAGE protein profiles of group VS116 and M19 strains were heterogeneous. Phylogenetic analysis of the partial 16s rRNA gene sequences showed that group VS116 and M19 spirochetes were members of a Borrelia species distinct from previously characterized members of the genus Borrelia. Based on our present study and data from previous DNA-DNA hybridizations, a new Borrelia species, Borrelia valaisiana sp. nov., in the B. burgdorferi complex, is proposed. Strain VS116 is the type strain of this new species.

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confidence: 99%

Genetic and Phenotypic Analysis of Borrelia valaisiana sp. nov. (Borrelia Genomic Groups VS116 and M19)

Wang¹,

Dam²,

Flèche

et al. 1997

International Journal of Systematic Bacteriology

192

131

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“…Borrelia burgdorferi sensu lato are classified into seven species: B. burgdorferi (7,13) sensu stricto isolated in North America and Europe; Borrelia garinii (3) and Borrelia afzelii (8) isolated in Europe and Japan; Borrelia japonica (14,25), Borrelia tanukii (10,17,19) and Borrelia trudae (10,17) isolated from Ixodes ovatus, Ixodes tanuki and Ixodes turdus in Japan; and Borrelia andersonii isolated from Ixodes dentatus in North America (15). Postic et al (24) developed a simple and rapid method for identification of species of B. burgdorferi sensu lato based on restriction fragment length polymorphism (RFLP) analysis of the 5S-23S ribosomal RNA (rRNA) intergenic spacer amplicon, and identified three genomic groups (Groups DN127, VS116 and Poti B2) among isolates from North America and Europe.…”

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confidence: 99%

Genetic Diversity of Borrelia burgdorferi Sensu Lato Isolated in Far Eastern Russia

Masuzawa

Iwaki

Sato

et al. 1997

Microbiology and Immunology

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One-hundred and fifty-seven Borrelia isolated from adult ticks, Ixodes persulcatus, and wild rodents, Clethrionomys rufocanus and Apodemus peninsulae, in the far eastern part of Russia were charaeterized and identified by restriction fragment length polymorphism (RFLP) of the 5S-23S rRNA intergenic spacer. Some isolates showed unique RFLP patterns and were determined as Borrelia garinii on the basis of a sequence analysis of the intergenic spacer amplicon and reactivity with species-specific monoclonal antibodies (MAbs). 86.5 and 12.7% of the tick isolates, and 74.2 and 12.9% of the rodent isolates were determined as Borrelia garinii and Borrelia afzelii, respectively, but no Borrelia burgdorferi sensu stricto was detected. This finding is similar to the results obtained from Borrelia surveys of I. persulcatus and wild rodents in Hokkaido, Japan.

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“…To date, a lot is known about the species composition of the whole complex of which three (perhaps four) species cause the disease with clinical symptoms typical of LB in different parts of the world. Based on gene sequence variation of a limited numbers of loci, the following 11 genospecies of B. burgdorferi sensu lato have so far been discriminated from each other and named: B. burgdorferi sensu stricto, B. garinii and B. afzelii (Baranton et al 1992, Canica et al 1993, B. japonica (Kawabata et al 1993), B. andersonii (Marconi et al 1995), B. tanukii, B. turdi (Fukunaga et al 1996), B. lusitaniae (LeFleche et al 1997), Borrelia bissettii (formerly genomic group DN127) ), B. valaisiana (Wang et al 1999, and the recently described B. sinica . Borrelia bissettii has been cultured from patients as an undocumented strain that is genetically similar to Borrelia japonica, but appears only occasionally in humans.…”

Section: Genospecies Of Borrelia Burgdorferi Sensu Latomentioning

confidence: 99%

Lyme borreliosis: insights into tick- / host-borrelia relations

Grubhoffer¹,

Golovchenko²,

Vancová³

et al. 2005

FOLIA PARASIT

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Abstract. Lyme borreliosis (LB) is a serious infectious disease of humans and some domestic animals in temperate regions of the Northern Hemisphere. It is caused by certain spirochetes in the Borrelia burgdorferi sensu lato (s.l.) species complex. The complex consists of 11 species (genospecies). Borrelia burgdorferi sensu stricto (s.s.), Borrelia garinii and Borrelia afzelii are the major agents of human disease. Borrelia burgdorferi s.l. species are transmitted mainly by ticks belonging to the Ixodes ricinus species complex plus a few additional species not currently assigned to the complex. B. burgdorferi infections may produce an acute or chronic disease with a wide array of clinical symptoms such as erythema migrans (EM), carditis, arthritis, neuroborreliosis, and acrodermatitis chronica atrophicans (ACA). Differences in LB spirochetes 'genospecies' and strains/isolates determine the occurrence and severity of this multi-system disease. Accurate and reliable identification of the LB spirochetes in ticks as well as knowledge of their prevalence are essential for prevention against the disease and development of an effective vaccine. An overview of the knowledge of molecular factors with emphasis on potential protein-carbohydrate interactions in the tickborrelia system is the main focus of this review.

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Borrelia tanukii Sp. Nov. and Borrelia turdae Sp. Nov. Found from Ixodid Ticks in Japan: Rapid Species Identification by 16S rRNA Gene‐Targeted PCR Analysis

Cited by 116 publications

References 25 publications

Genetic and Phenotypic Analysis of Borrelia valaisiana sp. nov. (Borrelia Genomic Groups VS116 and M19)

Genetic and Phenotypic Analysis of Borrelia valaisiana sp. nov. (Borrelia Genomic Groups VS116 and M19)

Genetic Diversity of Borrelia burgdorferi Sensu Lato Isolated in Far Eastern Russia

Lyme borreliosis: insights into tick- / host-borrelia relations

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