<i>Blastocladiella emersonii</i> expresses a centrin similar to <i>Chlamydomonas reinhardtii</i> isoform not found in late‐diverging fungi

Ribichich, Karina Fabiana; Gomes, Suely L.

doi:10.1016/j.febslet.2005.06.075

Cited by 6 publications

(2 citation statements)

References 36 publications

(44 reference statements)

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“…Accordingly, we hypothesized that the transcriptional levels of genes encoding axoneme-specific, as well as basal body-specific proteins should correspond to the level of zoosporogenesis. Therefore, in conjunction with the microscopic examination, we followed the transcriptional levels of RS3 (encoding an axoneme-specific protein [ 27 ]), and centrin (encoding basal body and nuclear cap-specific protein [ 28 ]) in the samples collected above (days 1-2-5-15-19). Samples with the highest transcriptional levels of RS3 and centrin genes and microscopic evidence of active zoosporogenesis were used as representatives of active sporangia, while late samples that showed no microscopic evidence of zoosporogenesis as well as very low to no transcription of RS3 and centrin were used as representatives of late sporangia samples.…”

Section: Methodsmentioning

confidence: 99%

Insights into the Utility of the Focal Adhesion Scaffolding Proteins in the Anaerobic Fungus Orpinomyces sp. C1A

Calkins

Youssef

2016

PLoS ONE

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Focal adhesions (FAs) are large eukaryotic multiprotein complexes that are present in all metazoan cells and function as stable sites of tight adhesion between the extracellular matrix (ECM) and the cell’s cytoskeleton. FAs consist of anchor membrane protein (integrins), scaffolding proteins (e.g. α-actinin, talin, paxillin, and vinculin), signaling proteins of the IPP complex (e.g. integrin-linked kinase, α-parvin, and PINCH), and signaling kinases (e.g. focal adhesion kinase (FAK) and Src kinase). While genes encoding complete focal adhesion machineries are present in genomes of all multicellular Metazoa; incomplete machineries were identified in the genomes of multiple non-metazoan unicellular Holozoa, basal fungal lineages, and amoebozoan representatives. Since a complete FA machinery is required for functioning, the putative role, if any, of these incomplete FA machineries is currently unclear. We sought to examine the expression patterns of FA-associated genes in the anaerobic basal fungal isolate Orpinomyces sp. strain C1A under different growth conditions and at different developmental stages. Strain C1A lacks clear homologues of integrin, and the two signaling kinases FAK and Src, but encodes for all scaffolding proteins, and the IPP complex proteins. We developed a protocol for synchronizing growth of C1A cultures, allowing for the collection and mRNA extraction from flagellated spores, encysted germinating spores, active zoosporangia, and late inactive sporangia of strain C1A. We demonstrate that the genes encoding the FA scaffolding proteins α-actinin, talin, paxillin, and vinculin are indeed transcribed under all growth conditions, and at all developmental stages of growth. Further, analysis of the observed transcriptional patterns suggests the putative involvement of these components in alternative non-adhesion-specific functions, such as hyphal tip growth during germination and flagellar assembly during zoosporogenesis. Based on these results, we propose putative alternative functions for such proteins in the anaerobic gut fungi. Our results highlight the presumed diverse functionalities of FA scaffolding proteins in basal fungi.

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Section: Methodsmentioning

confidence: 99%

Insights into the Utility of the Focal Adhesion Scaffolding Proteins in the Anaerobic Fungus Orpinomyces sp. C1A

Calkins

Youssef

2016

PLoS ONE

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“…Nas algas verdes, como, Chlamydomonas reinhardtii, localizam-se no centro organizador de microtúbulos e participam da contração de fibras conectoras do núcleo ao corpo basal, sendo este mecanismo dependente de cálcio (18). Também existem relatos da participação de centrinas na excisão do flagelo em C. reinhardtii (19 (29).…”

Section: Centrinasunclassified

Estudos estruturais e termodinâmicos de centrinas BeCen1 e BeCen3 do fungo >i<Blastocladiella emersonii>/i<

Camargo¹

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AGRADECIMENTOSPrimeiramente quero agradecer a minha querida família, que foi quem me forneceu todo o suporte necessário para chegar até aqui. Mãe, Pai, Angela e Marcia muito obrigado por sempre estarem presentes na minha vida, eu os amo infinitamente. Ao meu amado "namorido" Júlio que fez parte desses ótimos 4 anos de doutorado e contribuiu imensamente para meu crescimento. Ensinou-me muita coisa, promoveu momentos inesquecíveis, permitiu realização de sonhos e continua me fazendo feliz a cada dia! Amo você! Aos meus queridos cunhados, Evandro e Dio, obrigado por fazerem parte da família e aos meus amados e insubstituíveis sobrinhos: Kauet, Isabela, Inaiá e Henry obrigado por me ensinarem coisas novas sempre.Aos meus companheiros Sussu e Joci que foram meus grandes amigos de todas as horas, principalmente quando o assunto era "smirnoff" ou mesmo só um cafezinho... Voces dois foram essênciais para minha vida estes anos de doutorado, não posso descrever a alegria de ter convivido com vocês.Aos "antigos" (porém não velhos) amigos da biofísica, As minhas novas amizades da Enfermagem que tornam minhas noites mais prazerosas, proveitosas e por fazerem parte da realização de um sonho antigo.As minhas amadas amigas, que mesmo estando longe, fazem parte da minha vida: , pela oportunidade e orientação. Palavras-chave: Centrinas, Proteínas ligantes de cálcio, Formação de filamentos, Domínio "EF-Hand", Blastocladiella emersonii. ABSTRACT CAMARGO, A. I. Structural and Thermodynamics Studies of Blastocladiella Emersonii Centrins. 2011. 119p. Tese (Doutorado) -Instituto de Física de São Carlos, Universidade de São Paulo, São Carlos, 2011.Centrin proteins are essential component of the microtubule organizing centers in a large range of organisms. They belong to the EF-Hand calcium binding proteins family e can be divided in two subfamilies: one defined by the green algae Chlamydomonas reinhardtii CrCenp, related to contractile functions and the other centrin of the yeast Saccharomices cerevisiae ScCdc31p, related to duplication of centrossome mitotic centers. Interesantly, Blastocladiella emersonii fungus posses two centrin forms in it genome: BeCen1 closely related to CrCenp and BeCen3 closely related to ScCdc31p. All other known fungus have only one form of centrin: ScCdc31p. With this curious finding, described in 2005, compared structural and thermodynamics studies between recombinant proteins BeCen1 and BeCen3 were performed, in attempt to identify relevant differences that could explain the presence of two forms of centrins in this fungus. Both centrins were expressed in E. coli and purified by chromatography resulting in 5mg/l protein yield. Structural analyses were performed with circular dichroism (CD), fluorescence, light scattering (DLS AND RALS), atomic force microscopy (AFM) and transmission electronic microscopy (TEM). Using isothermal titration calorimetry (ITC) thermodynamics parameters about the calcium binding were defined. Both showed alpha helix predominant content and structural physical-chemistry difference...

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Origin and Evolution of the Centrosome

Bornens

Azimzadeh

Advances in Experimental Medicine and Biology

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In this brief account we specifically address the question of how the plasma membrane-associated basal body/axoneme of the unicellular ancestor of eukaryotes has evolved into the centrosome organelle through the several attempts to multicellularity. We propose that the connection between the flagellar apparatus and the nucleus has been a critical feature for leading to the centriole-based centrosome of metazoa, the Spindle Pole Body of fungi, or to the absence of any centrosome in seed plants. We further suggest that the evolution of this connection could be reflected in the evolution of the centrin proteins. We then review evidence showing that the evolution of the centrosome-based tubulin network has been correlated with the evolution of the cortical actin-based cleavage apparatus. Finally we argue that this coevolution had a major impact on the cell individuation process and on the evolution of multicellular organisms. We conclude that only the metazoan lineage evolved multicellularity without loosing the ancestral association of three basic cellular functions of the basal body/axoneme or the derived centrosome organelle, namely sensation, motion and division.

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Blastocladiella emersonii expresses a centrin similar to Chlamydomonas reinhardtii isoform not found in late‐diverging fungi

Cited by 6 publications

References 36 publications

Insights into the Utility of the Focal Adhesion Scaffolding Proteins in the Anaerobic Fungus Orpinomyces sp. C1A

Insights into the Utility of the Focal Adhesion Scaffolding Proteins in the Anaerobic Fungus Orpinomyces sp. C1A

Estudos estruturais e termodinâmicos de centrinas BeCen1 e BeCen3 do fungo >i<Blastocladiella emersonii>/i<

Origin and Evolution of the Centrosome

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