<i>Bifidobacterium longum</i>Suppresses Murine Colorectal Cancer through the Modulation of oncomiRs and Tumor Suppressor miRNAs

Fahmy, Cinderella A.; Gamal‐Eldeen, Amira M.; El‐Hussieny, Enas A.; Raafat, Bassem M.; Mehanna, Nayra; Talaat, Roba M.; Shaaban, Mohamed T.

doi:10.1080/01635581.2019.1577984

Cited by 53 publications

(35 citation statements)

References 36 publications

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“…Mechanisms by which bacteria confer protection against CRC include competition against pathogenic bacteria, metabolic functions, and genetic modulations [22]. In a colitis-induced murine model of CRC, administration of Bifidobacterium longum resulted in increased expression of tumor suppressor micro-RNAs (miRs) miR-145, and miR-15a, decreased expression of miR-146a (which regulates expression of interleukins 1β and 6), decreased nuclear factor kappa-light-chain-enhancer of activated B cell (NF-κB) activation, and resulted in decreased aberrant crypt foci numbers [25]. In another murine study, Bifidobacterium longum has also been reported to suppress food mutagen (2-Amino-3-methylimidazo(4,5-f)quinoline) induced colon cancer incidence [26].…”

Section: Bacteria and Protection Against Colorectal Cancermentioning

confidence: 99%

“…Patient samples: Tissue [9], Stool [10] Murine model of microbe-induced colon tumorigenesis [12] Increased abundance of ETBF in early-stage lesions [9] and stool samples [10] Bacteroides fragilis toxin (BFT) mediated increase in IL-17 dependent NF-κB activation, chemokines production and myeloid cell accumulation [12] BFT mediated tumorigenesis of colonic epithelial cells through mechanisms dependent on STAT3 activation, and IL-17 signaling mediated NF-κB activation, production of C-X-C chemokines, and recruitment of CXCR2-expressing myeloid cells [12] Streptococcus bovis/gallolyticus CRC tissues from patients with or without bacteremia [17] In vitro with human colonic epithelial cell line Caco-2; and rat model of azoxymethane-induced colon carcinogenesis [15] Concomitant colorectal tumors present in about 25…”

Section: Bacteroides Fragilismentioning

confidence: 99%

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Colorectal Cancer and Probiotics: Are Bugs Really Drugs?

Lamichhane

Maiolini

Alnafoosi

et al. 2020

Cancers

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Colorectal cancer (CRC) is one of the most common types of cancer worldwide. There are many factors that predispose a patient to the disease such as age, family history, ethnicity, and lifestyle. There are different genetic factors and diseases that also increase a person’s risk for developing CRC. Studies have found associations between gut microbiome and the risk for developing versus protection against CRC. Normal gut microbiome aid in daily functions of the human body such as absorption, metabolism, detoxification, and regulation of inflammation. While some species of bacteria prevent CRC development and aid in therapeutic responses to various treatment regiments, other species seem to promote CRC pathogenesis. In this regard, many studies have been conducted to not only understand the biology behind these opposing different bacterial species; but also to determine if supplementation of these tumor opposing bacterial species as probiotics lends toward decreased risk of CRC development and improved therapeutic responses in patients with CRC. In this literature review, we aim to discuss the basics on colorectal cancer (epidemiology, risk factors, targets, treatments), discuss associations between different bacterial strains and CRC, and discuss probiotics and their roles in CRC prevention and treatment.

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Section: Bacteria and Protection Against Colorectal Cancermentioning

confidence: 99%

Section: Bacteroides Fragilismentioning

confidence: 99%

Colorectal Cancer and Probiotics: Are Bugs Really Drugs?

Lamichhane

Maiolini

Alnafoosi

et al. 2020

Cancers

View full text Add to dashboard Cite

show abstract

“…As well, Fahmy et al revealed that treatment of CRC mice with B. longum , isolated from women breast milk, decrease NF-κB and IL-6 concentration. On the other hand, administration of this bacteria increased IL-1β concentration and resulted in the decline of aberrant crypt foci number in CRC-mice and improve necrosis and fibrosis of the colon cells [ 47 ]. Since the NF-κB is involved in cell proliferation and also plays a critical role in the inflammatory process, it provides a possible mechanistic link between inflammation and cancer [ 48 ].…”

Section: Discussionmentioning

confidence: 99%

Anticancer effects of bifidobacteria on colon cancer cell lines

Faghfoori

Saber

et al. 2021

Cancer Cell Int

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Background Colorectal cancer (CRC), with a growing incidence trend worldwide, is resistant to apoptosis and has uncontrolled proliferation. It is recently reported that probiotic microorganisms exert anticancer effects. The genus Bifidobacterium, one of the dominant bacterial populations in the gastrointestinal tract, has received increasing attention because of widespread interest in using it as health-promoting microorganisms. Therefore, the present study aimed to assess the apoptotic effects of some bifidobacteria species on colon cancer cell lines. Methods The cytotoxicity evaluations performed using MTT assay and FACS-flow cytometry tests. Also, the effects of five species of bifidobacteria secretion metabolites on the expression level of anti- or pro-apoptotic genes including BAD, Bcl-2, Caspase-3, Caspase-8, Caspase-9, and Fas-R studied by real-time polymerase chain reaction (RT-PCR) method. Results The cell-free supernatant of all studied bifidobacteria significantly decreased the survival rates of colon cancer cells compared with control groups. Flow cytometric and RT-PCR results indicated that apoptosis is induced by bifidobacteria secretion metabolites and the mechanism for the action of bifidobacteria species in CRC prevention could be down-regulation and up-regulation of anti-apoptotic and, pro-apoptotic genes. Conclusions In the present study, different bifidobacteria species showed anticancer activity on colorectal cancer cells through down-regulation and up-regulation of anti-apoptotic and pro-apoptotic genes. However, further studies are required to clarify the exact mechanism of apoptosis induction by bifidobacteria species.

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“…As well, Fahmy et al revealed that treatment of CRC mice with B. longum, isolated from women breast milk, decrease NF-κB and IL-6 concentration. On the other hand, administration of this bacteria increased IL-1β concentration and resulted in the decline of aberrant crypt foci number in CRC-mice and improve necrosis and brosis of the colon cells (47). Since the NF-κB is involved in cell proliferation and also plays a critical role in the in ammatory process, it provides a possible mechanistic link between in ammation and cancer (48).…”

Section: Discussionmentioning

confidence: 99%

Anticancer effects of Bifidobacteria on colon cancer cell lines

Faghfoori

Saber

et al. 2021

Preprint

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Background: Colorectal cancer (CRC), with a growing incidence trend worldwide, is resistant to apoptosis and have the uncontrolled proliferation. It is recently reported that probiotic microorganisms exert anticancer effects. The genus Bifidobacterium, one of the dominant bacterial populations in the gastrointestinal tract, has received increasing attention because of widespread interest in using as health-promoting microorganisms. Therefore, the present study aimed to assess the apoptotic effects of some bifidobacteria species on colon cancer cell lines.Methods: The cytotoxicity evaluations performed using MTT assay and FACS-flow cytometry tests. Also, the effects of five species of bifidobacteria secretion metabolites on the expression level of anti- or pro-apoptotic genes including BAD, Bcl-2, Caspase-3, Caspase-8, Caspase-9, and Fas-R studied by real-time polymerase chain reaction (RT-PCR) method. Results: The cell-free supernatant of all studied bifidobacteria significantly decreased the survival rates of colon cancer cells compared with control groups. Flow cytometric and RT-PCR results indicated that apoptosis is induced by bifidobacteria secretion metabolites and the mechanism for the action of bifidobacteria species in CRC prevention could be down-regulation and up-regulation of anti-apoptotic and, pro-apoptotic genes.Conclusions: In the present study, different bifidobacteria species showed anticancer activity on colorectal cancer cells through down-regulation and up-regulation of anti-apoptotic and pro-apoptotic genes. However, further studies are required to clarify the exact mechanism of apoptosis induction by bifidobacteria species.

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Bifidobacterium longumSuppresses Murine Colorectal Cancer through the Modulation of oncomiRs and Tumor Suppressor miRNAs

Cited by 53 publications

References 36 publications

Colorectal Cancer and Probiotics: Are Bugs Really Drugs?

Colorectal Cancer and Probiotics: Are Bugs Really Drugs?

Anticancer effects of bifidobacteria on colon cancer cell lines

Anticancer effects of Bifidobacteria on colon cancer cell lines

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