Bifidobacterium animalisssp.lactis420 Protects against Indomethacin-Induced Gastric Permeability in Rats

Abstract

<jats:p>Gastrointestinal (GI) adverse effects such as erosion and increased permeability are common during the use of nonsteroidal anti-inflammatory drugs (NSAIDs). Our objective was to assess whether<jats:italic>Bifidobacterium animalis</jats:italic>ssp.<jats:italic>lactis</jats:italic>420 protects against NSAID-induced GI side effects in a rat model. A total of 120 male Wistar rats were allocated into groups designated as control, NSAID, and probiotic. The NSAID and probiotic groups were challenged with indomethacin (10 mg/kg<jats:sup>−1</jats:sup>; single dose). The probiotic group was also supplemented daily with 10<jats:sup>10</jats:sup> CFU of<jats:italic>B. lactis</jats:italic>420 for seven days prior to the indomethacin administration. The control group rats received no indomethacin or probiotic. The permeability of the rat intestine was analysed using carbohydrate probes and the visual damage of the rat stomach mucosa was graded according to severity.<jats:italic>B. lactis</jats:italic>420 significantly reduced the indomethacin-induced increase in stomach permeability. However, the protective effect on the visual mucosal damage was not significant. The incidence of severe NSAID-induced lesions was, nevertheless, reduced from 50% to 33% with the probiotic treatment. To conclude, the<jats:italic>B. lactis</jats:italic>420 supplementation protected the rats from an NSAID-induced increase in stomach permeability and may reduce the formation of more serious GI mucosal damage and/or enhance the recovery rate of the stomach mucosa.</jats:p>

Bifidobacterium animalis subsp. lactis 420 for Metabolic Health: Review of the Research

“…Schematic proposal of how B420 and/or its metabolites presumably affect cellular signaling via downregulation of TLR pathways, indicated as light green arrows in the figure, as well as the inflammatory IKKα-NF-κB pathways or p38 MAPK-ELK-1 pathways. B420 has been shown to enhance epithelial integrity in vitro [22] and to decrease the levels of circulating LPS in mice [27]. LPS triggers inflammation through upregulation of IKKα-NF-κB pathways and p38 MAPK-ELK-1 pathways [72], indicated as dark green arrows in the figure.…”

“…There is growing evidence on the ability of B420 to affect weight and metabolism via gut barrier function and gut microbiota composition modulation in various in vitro and in vivo trials, as well as in a clinical study. An important feature of B420 in weight maintenance can be related to its ability to enhance intestinal epithelial integrity in vitro [22] and in vivo [27]. Furthermore, in obesogenic mouse models, B420 feeding has been shown to decrease in the quantity of inflammatory markers and gut-derived bacteria in tissues [23,25].…”

“…In an animal study, treatment with a strain of B. lactis was able to suppress COX-2 expression and colonic TNF-α production in a trinitrobenzene-sulfonic acid-induced model of rat colitis [81]. Furthermore, in a rat model, B420 supplementation protected from an NSAID-induced increase in gastric permeability [27]. B420 has been shown to affect the COX pathway by producing metabolites that have been observed to upregulate COX-1 in an undifferentiated and differentiated human intestinal epithelial cell model, Caco-2, and concomitantly, downregulate the expression of COX-2 [22,82].…”

Effect of NSAIDs on Na+/H+ exchanger activity in rat colonic crypts

“…Furthermore, high-dose aspirin and indomethacin target COX-1 and COX-2, both of which synthesize proinflammatory prostaglandins (35,43,45). COX-2 activity is inducible and has been implicated in the progression of conditions such as osteoarthritis through induction of hyperalgesia and inflammation (32,43).…”

MICROENCAPSULATION OF BIFIDOBACTERIUM LACTIS Bi-07 WITH GALACTOOLIGOSACCHARIDES USING CO-EXTRUSION TECHNIQUE

“…lactis which are gram-positive, non-spore forming, lactic acid-producing bacteria (Morovic et al, 2017). B. lactis Bi-07 is a lactic acid bacteria that tend to have a higher abundance in colonic microbiota (Lyra et al, 2012). It has been widely studied for its beneficial effect on gastrointestinal and colon health.…”