<i>Betula platyphylla BpHOX2</i> transcription factor binds to different cis‐acting elements and confers osmotic tolerance

Tan, Zilong; Wen, Xuejing; Wang, Yucheng

doi:10.1111/jipb.12994

Cited by 28 publications

(20 citation statements)

References 46 publications

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“…Proline is reported to act as both an osmolyte and an ROS scavenger, alleviating damage caused by various stresses [ 50 ]. Overexpression of Betula platyphylla homeodomain-leucine zipper I class homeobox gene ( BpHOX2 ) improved birch plants osmotic tolerance partly by promoting the biosynthesis of proline [ 51 ]. Here, in addition to its function in osmotic adjustment, proline may also have acted as a hydroxy radical scavenger to protect the photosynthetic apparatus under drought in OE lines.…”

Section: Discussionmentioning

confidence: 99%

Overexpression of MdATG8i Enhances Drought Tolerance by Alleviating Oxidative Damage and Promoting Water Uptake in Transgenic Apple

Gong

Jia

et al. 2021

IJMS

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Water deficit adversely affects apple (Malus domestica) productivity on the Loess Plateau. Autophagy plays a key role in plant responses to unfavorable environmental conditions. Previously, we demonstrated that a core apple autophagy-related protein, MdATG8i, was responsive to various stresses at the transcript level. Here, we investigated the function of this gene in the response of apple to severe drought and found that its overexpression (OE) significantly enhanced drought tolerance. Under drought conditions, MdATG8iOE apple plants exhibited less drought-related damage and maintained higher photosynthetic capacities compared with the wild type (WT). The accumulation of ROS (reactive oxygen species) was lower in OE plants under drought stress and was accompanied by higher activities of antioxidant enzymes. Besides, OE plants accumulated lower amounts of insoluble or oxidized proteins but greater amounts of amino acids and flavonoid under severe drought stress, probably due to their enhanced autophagic activities. Particularly, MdATG8iOE plants showed higher root hydraulic conductivity than WT plants did under drought conditions, indicating the enhanced ability of water uptake. In summary, the overexpression of MdATG8i alleviated oxidative damage, modulated amino acid metabolism and flavonoid synthesis, and improved root water uptake, ultimately contributing to enhanced drought tolerance in apple.

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Section: Discussionmentioning

confidence: 99%

Overexpression of MdATG8i Enhances Drought Tolerance by Alleviating Oxidative Damage and Promoting Water Uptake in Transgenic Apple

Gong

Jia

et al. 2021

IJMS

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“…Each sample contained at least 9 seedlings, and three independent experiments were performed. qRT-PCR was used to detect the relative expression levels of five ABA biosynthesis-related genes (NCED1, 3, 4, ZEP, and AAO3) (Tan et al, 2020) in OE transgenic plants after normal condition and stress with 150 mM NaCl for 24 h. The primers used for qRT-PCR are listed in Supplementary Table 1.…”

Section: Biochemical Staining and Physiological Measurements Of Transformed Plantsmentioning

confidence: 99%

ThCOL2 Improves the Salt Stress Tolerance of Tamarix hispida

et al. 2021

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The CONSTANS-LIKE (COL) transcription factor has been reported to play important roles in regulating plant flowering and the response to abiotic stress. To clone and screen COL genes with excellent salt tolerance from the woody halophyte Tamarix hispida, 8 ThCOL genes were identified in this study. The expression patterns of these genes under different abiotic stresses (high salt, osmotic, and heavy metal) and abscisic acid (ABA) treatment were detected using quantitative real-time PCR (qRT-PCR). The expression levels of 8 ThCOL genes changed significantly after exposure to one or more stresses, indicating that these genes were all stress-responsive genes and may be involved in the stress resistance response of T. hispida. In particular, the expression level of ThCOL2 changed significantly at most time points in the roots and leaves of T. hispida under salt stress and after ABA treatments, which may play an important role in the response process of salt stress through a mechanism dependent on the ABA pathway. The recombinant vectors pROKII–ThCOL2 and pFGC5941–ThCOL2 were constructed for the transient transformation of T. hispida, and the transient infection of T. hispida with the pROKII empty vector was used as the control to further verify whether the ThCOL2 gene was involved in the regulation of the salt tolerance response of T. hispida. Overexpression of the ThCOL2 gene in plants under 150 mM NaCl stress increased the ability of transgenic T. hispida cells to remove reactive oxygen species (ROS) by regulating the activity of protective enzymes and promoting a decrease in the accumulation of O2– and H2O2, thereby reducing cell damage or cell death and enhancing salt tolerance. The ThCOL2 gene may be a candidate gene associated with excellent salt tolerance. Furthermore, the expression levels of some genes related to the ABA pathway were analyzed using qRT-PCR. The results showed that the expressions of ThNCED1 and ThNCED4 were significantly higher, and the expressions of ThNCED3, ThZEP, and ThAAO3 were not significantly altered in OE compared with CON under normal conditions. But after 24 h of salt stress, the expressions of all five studied genes all were lower than the normal condition. In the future, the downstream genes directly regulated by the ThCOL2 transcription factor will be searched and identified to analyze the salt tolerance regulatory network of ThCOL2.

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“…ROS accumulation depends largely on the balance between ROS production and simultaneous ROS scavenging (Liu et al, 2019 SOD and POD play a central part (Wei et al, 2019). Previous studies have shown that expression of BpHOX2 (which encodes an HD-Zip I family protein) increased POD and SOD activities to scavenge excess ROS, ultimately reducing ROS levels and enhancing osmotic stress tolerance (Tan et al, 2020). In this work, POD and SOD enzyme activities under salt stress were significantly higher in MdHB7-like OE lines and lower in RNAi lines compared with GL-3 plants (Figure 6).…”

Section: Discussionmentioning

confidence: 99%

The HD‐Zip I transcription factor MdHB7‐like confers tolerance to salinity in transgenic apple (Malus domestica)

Zhao

Wang

Jia

et al. 2021

Physiologia Plantarum

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Salinity is a major environmental constraint that substantially limits global agricultural productivity. HD-Zip I transcription factors are involved in plant responses to salt stress, but little is known about the HD-Zip I genes in apple (Malus domestica). Here, we characterized the function of an apple HD-Zip I gene (MdHB7-like) and report that its expression is induced by salt stress. To further explore its role in salt stress, we created MdHB7-like overexpressing and RNAi transgenic apple plants. The overexpression of MdHB7-like improved the photosynthetic performance and reduced ROS and Na + accumulation under salt stress. Plants that overexpressed MdHB7-like also showed increased accumulation of proline and soluble sugars, which may have played an important role in their salt stress tolerance. RNAi suppression of MdHB7-like had the opposite effects. Together, our results demonstrate that MdHB7-like is an important regulator of salt tolerance in apple. Our results provide new insights for future research on the mechanisms by which MdHB7-like promotes salt tolerance and provide a potential target for molecular breeding in apple.

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Betula platyphylla BpHOX2 transcription factor binds to different cis‐acting elements and confers osmotic tolerance

Cited by 28 publications

References 46 publications

Overexpression of MdATG8i Enhances Drought Tolerance by Alleviating Oxidative Damage and Promoting Water Uptake in Transgenic Apple

Overexpression of MdATG8i Enhances Drought Tolerance by Alleviating Oxidative Damage and Promoting Water Uptake in Transgenic Apple

ThCOL2 Improves the Salt Stress Tolerance of Tamarix hispida

The HD‐Zip I transcription factor MdHB7‐like confers tolerance to salinity in transgenic apple (Malus domestica)

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