I Believe I Can Fly!: Use of Drosophila as a Model Organism in Neuropsychopharmacology Research

Narayanan, Anjana S.; Rothenfluh, Adrian

doi:10.1038/npp.2015.322

Cited by 29 publications

(30 citation statements)

References 72 publications

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“…The fruit fly ( Drosophila melanogaster ) is a well-established model species for studying the neuronal circuits involved in sensory perception (Albert and Göpfert, 2015 ; Behnia and Desplan, 2015 ; Joseph and Carlson, 2015 ) and a wide range of behaviors (Owald and Waddell, 2015 ; Anderson, 2016 ; Auer and Benton, 2016 ; Dubowy and Sehgal, 2017 ). Drosophila has also become an attractive model for brain pathologies and disorders (McGurk et al, 2015 ; Narayanan and Rothenfluh, 2016 ), aging and age related decline (Jones and Grotewiel, 2011 ) or addictions (Kaun et al, 2012 ). A key advantage of Drosophila is the availability of a large collection of transgenic constructs for monitoring or altering neuronal activity (Venken et al, 2011 ; Sivanantharajah and Zhang, 2015 ).…”

Section: Introductionmentioning

confidence: 99%

A Statistically Representative Atlas for Mapping Neuronal Circuits in the Drosophila Adult Brain

Arganda‐Carreras

Manoliu

Mazuras

et al. 2018

Front. Neuroinform.

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Imaging the expression patterns of reporter constructs is a powerful tool to dissect the neuronal circuits of perception and behavior in the adult brain of Drosophila, one of the major models for studying brain functions. To date, several Drosophila brain templates and digital atlases have been built to automatically analyze and compare collections of expression pattern images. However, there has been no systematic comparison of performances between alternative atlasing strategies and registration algorithms. Here, we objectively evaluated the performance of different strategies for building adult Drosophila brain templates and atlases. In addition, we used state-of-the-art registration algorithms to generate a new group-wise inter-sex atlas. Our results highlight the benefit of statistical atlases over individual ones and show that the newly proposed inter-sex atlas outperformed existing solutions for automated registration and annotation of expression patterns. Over 3,000 images from the Janelia Farm FlyLight collection were registered using the proposed strategy. These registered expression patterns can be searched and compared with a new version of the BrainBaseWeb system and BrainGazer software. We illustrate the validity of our methodology and brain atlas with registration-based predictions of expression patterns in a subset of clock neurons. The described registration framework should benefit to brain studies in Drosophila and other insect species.

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Section: Introductionmentioning

confidence: 99%

A Statistically Representative Atlas for Mapping Neuronal Circuits in the Drosophila Adult Brain

Arganda‐Carreras

Manoliu

Mazuras

et al. 2018

Front. Neuroinform.

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“…[31] Exactly, Drosophila melanogaster is an excellent animal model to study the molecular and physiological mechanisms of MDD. The history of the use of Drosophila in modern biological sciences is a rich one, spanning more than a century.…”

Section: Discussionmentioning

confidence: 99%

The involvement of potassium channel ORK1 in short-term memory and sleep in Drosophila

Zhang

Zheng²,

Ren³

et al. 2017

Medicine

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Background:The sleep and cognitive dysfunction are common in major depressive disorders (MDDs). Recently, the 2-pore domain potassium channel twik-related K(+) channel 1 (TREK-1) has been identified to be closely related to the etiology of MDD. However, whether TREK-1 is involved in the regulation of sleep and cognition is still unknown.Methods:The present study tried to dissect the role of outwardly rectifying K+ channel-1 (ORK1) (TREK-1 homolog in Drosophila) in sleep and cognition in Drosophila. The mutant and over-expressed lines of ork1 were generated using Drosophila genetics. Sleep analysis and short-term memory experiments were used to test sleep time and short-term memory of the mutant and over-expressed ORK1 lines, respectively.Results:Our results showed that the learning index of ork1 mutant lines was increased compared with the wild type. However, ork1 mutant could obviously decrease sleep time in Drosophila. Contrary to the ork1 mutant lines, we also found that ORK1 over-expression could increase sleep time and decreased learning index in Drosophila.Conclusion:Results from this study suggest that ORK1 might play an important role in the regulation of sleep time and short-term memory in Drosophila.

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“…Of particular relevance to this report: RhoGAP18B (encoded by the whir gene) binds to, and acts on Rac1. 11 , 40 Rac1 is linked to Arf6 via Arfaptin, which binds to either activated GTPase. 21 Here, we show that Efa6 is required for Arf6 activation and behavioral ethanol responses, and together, these biochemical data support our initial finding of a genetic interaction between whir and Efa6 , placing them in the same network.…”

Section: Figurementioning

confidence: 99%

“…Numerous model organisms have been established to investigate genes and mechanisms that underlie the development of AUDs. Upon acute alcohol exposure, the vinegar fly, Drosophila melanogaster , exhibits behaviors similar to mammals, 11 , 12 such as disinhibition 13 and locomotor hyperactivity, 14 followed by sedation. 14 Repeat exposures can lead to rapid or chronic tolerance, i.e.…”

Section: Introductionmentioning

confidence: 99%

The Arf6 activator Efa6/PSD3 confers regional specificity and modulates ethanol consumption in Drosophila and humans

et al. 2017

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SUMMARYUbiquitously expressed genes have been implicated in a variety of specific behaviors, including responses to ethanol. However, the mechanisms that confer this behavioral specificity have remained elusive. Previously, we showed that the ubiquitously expressed small GTPase Arf6 is required for normal ethanol-induced sedation in adult Drosophila. Here, we show that this behavioral response also requires Efa6, one of (at least) three Drosophila Arf6 guanine exchange factors. Ethanol-naïve Arf6 and Efa6 mutants were sensitive to ethanol-induced sedation and lacked rapid tolerance upon re-exposure to ethanol, when compared to wild-type flies. In contrast to wild type-flies, both Arf6 and Efa6 mutants preferred alcohol-containing food without prior ethanol experience. An analysis of the human ortholog of Arf6 and orthologs of Efa6 (PSD1-4) revealed that the minor G-allele of SNP rs13265422 in PSD3, as well as a haplotype containing rs13265422, were associated with increased frequency of drinking and binge drinking episodes in adolescents. The same haplotype was also associated with increased alcohol dependence in an independent European cohort. Unlike the ubiquitously expressed human Arf6 GTPase, PSD3 localization is restricted to the brain, particularly the prefrontal cortex (PFC). Functional magnetic resonance imaging (fMRI) revealed that the same PSD3 haplotype was also associated with a differential fMRI signal in the PFC during a Go/No-Go task, which engages PFC-mediated executive control. Our translational analysis therefore suggests that PSD3 confers regional specificity to ubiquitous Arf6 in the PFC to modulate human alcohol-drinking behaviors.

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I Believe I Can Fly!: Use of Drosophila as a Model Organism in Neuropsychopharmacology Research

Cited by 29 publications

References 72 publications

A Statistically Representative Atlas for Mapping Neuronal Circuits in the Drosophila Adult Brain

A Statistically Representative Atlas for Mapping Neuronal Circuits in the Drosophila Adult Brain

The involvement of potassium channel ORK1 in short-term memory and sleep in Drosophila

The Arf6 activator Efa6/PSD3 confers regional specificity and modulates ethanol consumption in Drosophila and humans

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