Beauveria bassiana: a new N⁶-(2-hydroxyethyl)-adenosine–producing fungus

Abstract

N6-(2-Hydroxyethyl)-adenosine (HEA), which was the first calcium antagonist derived from biological sources, has been intensively investigated because of its ability to inhibit tumour cell proliferation, restrain inflammation, protect kidneys and function as a sedative and insecticide. Up to now, the production of HEA has been detected only in a few species such as Cordyceps pruinosa, C. militaris and Isaria tenuipes. Here, we found a new HEA-producing fungus, which was identified as Beauveria bassiana based on morphological and phylogenetic characteristics. The HEA production was verified by reversed-phase high-performance liquid chromatography/mass spectrometry in this fungus. Moreover, HEA was also detected in the mycelia of two other B. bassiana strains from different origins, but not in the culture medium of all tested B. bassiana strains. The maximum production of HEA (0.8483 ± 0.0439 mg/g mycelia dry weight) was achieved on day 7. The fungal biomass and kinetics of HEA production exhibited similar trends over the duration of the culture period. This is the first report describing HEA production in B. bassiana, which suggests that this fungal strain may have new applications as a source of HEA.

Secondary metabolites from hypocrealean entomopathogenic fungi: novel bioactive compounds

“…Its production was reported in several Cordyceps sensu lato species, and later in strains of Beauveria bassiana. 183 Two alloxazines, the novel analogue 1-methyl-11-hydroxylumichrome (250), along with lumichrome, a well-known riboflavin (vitamin B 2 ) derivative, were isolated from Beauveria bassiana mycelia cultured in rice-oat medium. 63 Lumichrome had previously been described as a signaling molecule produced by the bacterium Sinorhizobium meliloti that enhances root respiration in alfalfa (Medicago sativa) and triggers a compensatory increase in wholeplant net carbon assimilation at a dosage as low as 3 nM.…”

Promotion of Metabolite Synthesis in Isaria cicadae, a Dominant Species in the Cicada Flower Microbiota, by Cicada Pupae

“…HEA is a new nucleoside secondary metabolite discovered from the mycelia of Cordyceps and Isaria species. It is the first biologically derived Ca 2+ antagonist, which has been a research hotspot in the field of Cordyceps fungi in recent years. ,,− The two nucleosides are mainly produced by Cordyceps fungi through fermentation and solid-state culture . Therefore, the contents of adenosine and HEA were the main focus in our study.…”

Analysis of the bacteria community in wild Cordyceps cicadae and its influence on the production of HEA and nucleosides in Cordyceps cicadae

“…Therefore, we propose that the inhibition is related to the increased HEA yield. In industrial processes, HEA is usually produced by chemosynthesis and fermentation, but high cost and low yield are challenges of this production process (Qu et al 2006;Meng et al 2014;Fang et al 2016;Liu et al 2017a). Therefore, appropriate technology and methods of promoting the yield of HEA are essential for HEA development (Wang et al 2013;Kuan-Bo et al 2017).…”

“…HEA also attenuates lipopolysaccharide‐induced proinflammatory responses by suppressing the Toll‐like receptor 4‐mediated nuclear factor‐κB signalling pathway and protecting mice from renal ischaemia reperfusion injury (Lu et al ; Peng et al ). In the industrial production of HEA, there are still many problems that need to be solved, such as its high cost and low yield (Qu et al ; Meng et al ; Fang et al ; Liu et al ). Therefore, finding an efficient method to produce HEA is essential for its development.…”