Bacillus thuringiensis<scp>CHGP12</scp> uses a multifaceted approach for the suppression of Fusarium oxysporum f. sp. ciceris and to enhance the biomass of chickpea plants

Fatima, Rida; Mahmood, Tahir; Moosa, Anam; Aslam, Muhammad; Shakeel, Muhammad Taimoor; Maqsood, Ambreen; Shafiq, Muhammad Umar; Ahmad, Tanvir; Moustafa, Mahmoud; Alshehri, Mohammad

doi:10.1002/ps.7203

Cited by 19 publications

(9 citation statements)

References 61 publications

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“…The production of secondary metabolites with broad antimicrobial activity and highly differentiated structures are some of the interesting properties of Bacillus spp. [48][49][50][51]. Bacillus strains with significant potential to produce antifungal compounds are an important research field.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of Bacillus subtilis Czk1 Metabolites by LC–MS/MS and Their Antifungal Potential against Pyrrhoderma noxium Causing Brow Rot Disease

Liang

et al. 2023

Agriculture

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Brown rot disease caused by Pyrrhoderma noxium is a widespread disease that severely affects the roots of rubber trees (Hevea brasiliensis Muell. Arg.). The economic losses, along with environmental and health problems arising from the use of disease control chemicals, have raised the interest of scholars to explore the use of biological control agents for the effective control of fungal pathogen P. noxium. Here, the inhibition effect of the culture filtrate of B. subtilis Czk1 on P. noxium was demonstrated. The findings indicate that the antifungal activity of this strain is mediated wholly or partly by compounds produced in the culture filtrate. The combined use of liquid chromatography–tandem mass spectrometry and antifungal activity assays rapidly identified compounds produced by B. subtilis Czk1. Metabolic profiles were assessed and used to identify major metabolites based on the scores of variable importance in the projection and the plot scores of principal component analysis. A total of 296 differential metabolites were screened, including 208 in positive ion mode and 88 in negative ion mode. Two key metabolites, diacetyl and trans-2-octenoic acid, were screened from 29 metabolites by antifungal activity assays. The median effective concentration (EC50) of trans-2-octenoic acid and diacetyl were 0.9075 mg/mL and 4.8213 mg/mL, respectively. The antifungal metabolites can disrupt the internal structure of the pathogenic fungal mycelium, thereby impeding its growth. This study is expected to contribute to the existing knowledge of Czk1-produced metabolites and their future antifungal applications. This study is also expected to provide a new biopreservative perspective on unexplored antifungal metabolites produced by Czk1 as a biocontrol agent.

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Section: Discussionmentioning

confidence: 99%

Evaluation of Bacillus subtilis Czk1 Metabolites by LC–MS/MS and Their Antifungal Potential against Pyrrhoderma noxium Causing Brow Rot Disease

Liang

et al. 2023

Agriculture

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“…Many antagonistic bacteria secrete hydrolytic enzymes such as chitinase, glucanase, and protease, which plays a major role in fungal suppression ( Fatima et al, 2023 ; Xu et al, 2016 ). For example, the proteolytic activity of antagonistic bacteria exhibits strong inhibitory effect on fungal mycelial growth and conidial germination ( Gandhi Pragash et al, 2009 ; Ji et al, 2020 ; Ling et al, 2022 ).…”

Section: Discussionmentioning

confidence: 99%

Biological Control of Fusarium oxysporum, the Causal Agent of Fusarium Basal Rot in Onion by Bacillus spp.

Shin,

Lee,

Lee

et al. 2023

Plant Pathol J

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Fusarium oxysporum is the main pathogen causing Fusarium basal rot in onion (Allium cepa L.), which incurs significant yield losses before and after harvest. Among management strategies, biological control is an environmentally safe and sustainable alternative to chemical control. In this study, we isolated and screened bacteria for antifungal activity against the basal rot pathogen F. oxysporum. Isolates 23-045, 23-046, 23-052, 23-055, and 23-056 significantly inhibited F. oxysporum mycelial growth and conidial germination. Isolates 23-045, 23-046, 23-052, and 23-056 suppressed the development of Fusarium basal rot in both onion seedlings and bulbs in pot and spray inoculation assays. Isolate 23-055 was effective in onion seedlings but exhibited weak inhibitory effect on onion bulbs. Based on analyses of the 16S rRNA and rpoB gene sequences together with morphological analysis, isolates 23-045, 23-046, 23-052, and 23-055 were identified as Bacillus thuringiensis, and isolate 23-056 as Bacillus toyonensis. All five bacterial isolates exhibited cellulolytic, proteolytic, and phosphate-solubilizing activity, which may contribute to their antagonistic activity against onion basal rot disease. Taken together B. thuringiensis 23-045, 23-046, 23-052, and 23-055 and B. toyonensis 23-056 have potential for the biological control of Fusarium basal rot in onion.

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“…Bacillus strains were obtained from the bacterial culture collection in the Molecular Plant Pathology Laboratory at The Islamia University, Bahawalpur, Pakistan ( Table 1 ; Fatima et al, 2023 ), which were stored in a 60% glycerol stock solution at −80°C. The bacterial cultures were grown on fresh Luria Bertani (LB) medium, and incubated at 37°C.…”

Section: Methodsmentioning

confidence: 99%

Endophytic Bacillus atrophaeus CHGP13 and salicylic acid inhibit blue mold of lemon by regulating defense enzymes

et al. 2023

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Lemons (Citrus limon L.) are one of the most economically important and consumed fruit worldwide. The species is vulnerable to several postharvest decay pathogens, of which Penicillium italicum associated with blue mold disease is the most damaging. This study investigates the use of integrated management for blue mold of lemon using lipopeptides (LPs) extracted from endophytic Bacillus strains and resistance inducers. Two resistance inducers; salicylic acid (SA) and benzoic acid (BA) were tested at 2, 3, 4, and 5 mM concentrations against the development of blue mold on lemon fruit. The 5 mM SA treatment produced the lowest disease incidence (60%) and lesion diameter (1.4 cm) of blue mold on lemon fruit relative to the control. In an in vitro antagonism assay eighteen Bacillus strains were evaluated for their direct antifungal effect against P. italicum; CHGP13 and CHGP17 had the greatest inhibition zones of 2.30 and 2.14 cm. Lipopeptides (LPs) extracted from CHGP13 and CHGP17 also inhibited the colony growth of P. italicum. LPs extracted from CHGP13 and 5 mM SA were tested as single and combined treatments against disease incidence and lesion diameter of blue mold on lemon fruit. SA + CHGP13 + PI had the lowest disease incidence (30%) and lesion diameter (0.4 cm) of P. italicum on lemon fruit relative to the other treatments. Furthermore, the lemon fruit treated with SA + CHGP13 + PI had the highest PPO, POD, and PAL activities. The postharvest quality analysis of the lemon fruit including fruit firmness, total soluble solids, weight loss, titratable acidity, and ascorbic acid content revealed that the treatment SA + CHGP13 + PI had little effect on fruit quality compared to the healthy control. These findings indicate that Bacillus strains and resistance inducers can be used as components of integrated disease management for the blue mold of lemon.

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Bacillus thuringiensisCHGP12 uses a multifaceted approach for the suppression of Fusarium oxysporum f. sp. ciceris and to enhance the biomass of chickpea plants

Cited by 19 publications

References 61 publications

Evaluation of Bacillus subtilis Czk1 Metabolites by LC–MS/MS and Their Antifungal Potential against Pyrrhoderma noxium Causing Brow Rot Disease

Evaluation of Bacillus subtilis Czk1 Metabolites by LC–MS/MS and Their Antifungal Potential against Pyrrhoderma noxium Causing Brow Rot Disease

Biological Control of Fusarium oxysporum, the Causal Agent of Fusarium Basal Rot in Onion by Bacillus spp.

Endophytic Bacillus atrophaeus CHGP13 and salicylic acid inhibit blue mold of lemon by regulating defense enzymes

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