Bacillus subtilis chassis in biomanufacturing 4.0

Abstract

Synthetic biology, an emerging research field, can promote biomanufacturing 4.0 by offering various efficient chassis. Engineering Bacillus subtilis, a prominent workhorse in industrial biotechnology, through synthetic biology approaches may be a disruptive innovation. Diverse chassis forms are envisaged to deal with challenges in metabolic complexity, metabolic burden, and systems robustness. Thus, advancements in chassis engineering, a synthetic biology strategy for genome-reduced cell factories, cell-free systems, and synthetic microbial consortia would be a driving force facilitating microbial production. In this review article, we summarized chassis engineering categories and applications for B. subtilis. Prospects and challenges for chassis engineering in B. subtilis were also discussed.

Toward Minimal Transcription–Translation Machinery

“…11 In addition, the gene knockout does not damage the growth of strains and still preserves their core cellular processes, such as DNA replication, cell division, and gene expression. 12,13 Escherichia coli is the preferred host cell for protein expression, with the advantages of simple culture conditions, high protein yield, and low culture cost. Hashimoto et al reported the largest reduction of E. coli genome based on MG1655 strain, and constructed the mutant ΔMG1655 with 29.7% genome deletion.…”

“…By eliminating nonessential genes, genome-reduced strains reduce unnecessary components, DNA fragments, and useless surface structures in the system . In addition, the gene knockout does not damage the growth of strains and still preserves their core cellular processes, such as DNA replication, cell division, and gene expression. , Escherichia coli is the preferred host cell for protein expression, with the advantages of simple culture conditions, high protein yield, and low culture cost. Hashimoto et al reported the largest reduction of E.…”