<i>Bacillus subtilis</i>
            Biofilms: a Matter of Individual Choice

Ryan-Payseur, Bridgett K.; Freitag, Nancy E.

doi:10.1128/mbio.02339-18

Cited by 11 publications

(11 citation statements)

References 7 publications

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“…It has been reported that Bacillus subtilis has the capacity to choose at the individual cell level between biofilm formation and flagellum-mediated swimming motility with bacterial cells in a population expressing genes required for biofilm formation or genes required for swimming motility but not both. The decision to form either biofilm or free swimming cells by B. subtilis is mediated by a bistable switch controls called "bet hedging" that ensures that subpopulations of bacteria continue to grow as conditions change and/or become unfavorable (Ryan-Payseur and Freitag, 2018). The various processes and stages involved in biofilm formation by B. subtilis have been described (Gingichashvili et al, 2017;Ryan-Payseur and Freitag, 2018;Arnaouteli et al, 2021).…”

Section: Resultsmentioning

confidence: 99%

“…The decision to form either biofilm or free swimming cells by B. subtilis is mediated by a bistable switch controls called "bet hedging" that ensures that subpopulations of bacteria continue to grow as conditions change and/or become unfavorable (Ryan-Payseur and Freitag, 2018). The various processes and stages involved in biofilm formation by B. subtilis have been described (Gingichashvili et al, 2017;Ryan-Payseur and Freitag, 2018;Arnaouteli et al, 2021). One important attribute of biofilm that encourages their persistence is the reduced susceptibility to antimicrobials including antibiotics, disinfectants and antiseptics (Stewart, 2015).…”

Section: Resultsmentioning

confidence: 99%

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Periplaneta americana: Antibiotic susceptibility profiles of associated biofilm-forming bacteria

Osungunna¹,

Nwuga²

2022

njpr

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Background: The high resistance attributed to biofilms can result in recurrence and persistence of infections with attendant consequences of increased morbidity and mortality rates, increased cost of treatment as well as length of hospital stay of the patient.Objectives: This study aimed at examining the biofilm-forming capacity of bacterial isolates from the external body of cockroaches (Periplaneta americana) and their susceptibility to selected commonly used antibiotics.Methods: Bacterial isolates associated with seventy (70) cockroaches were isolated, identified and characterized using morphology and conventional biochemical tests. The biofilm-forming capacity of the isolates was evaluated using the Congo red agar (CRA) method. The antibiotic susceptibility profiles of the CRA-positive isolates to selected commonly used antibiotics were evaluated using the Kirby-Bauer disc diffusion method.Results: Of the one hundred and four (104) isolates, Bacillus subtilis was the predominant bacterial species (77.9%) while the least was Salmonella typhi (1.0%). However, 42% of the isolates showed tendency to form biofilms. The susceptibility study revealed that gentamicin was active against both Gram-positive and negative biofilm-formers.Conclusion: This study concluded that cockroaches (Periplaneta americana) can habour some bacterial species capable of forming biofilms which may adversely affect public health.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Periplaneta americana: Antibiotic susceptibility profiles of associated biofilm-forming bacteria

Osungunna¹,

Nwuga²

2022

njpr

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“…B. subtilis is a common soil-dwelling bacterium typically associated with plant roots, forming versatile biofilms with heterogeneous subpopulations. The subpopulations use a bet-hedging strategy to choose between either biofilm formation or flagella-mediated motility (43). In this study, we have characterized the importance of NaCl as a chemical cue and its potential influence the bistable switch that controls the biofilm (sessile) versus flagella-mediated motility decision.…”

Section: Discussionmentioning

confidence: 99%

NaCl Triggers the Sessile-to-Motile Transition of Bacillus subtilis

Murugan

Gupta

Sankar

et al. 2022

Preprint

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Various chemical cues are known to alter the motile and sessile states of bacteria differentially and, in turn, the formation of biofilms. However, the underlying mechanisms at the cellular and molecular level remain less understood, which severely limits our ability to control biofilms. Here, we systematically studied the effects of NaCl on the dynamics of biofilm formation across various length scales and the associated changes in the regulation of gene expression in an undomesticated natural isolate of Bacillus subtilis. Interestingly, NaCl induced significant changes in the architecture of pellicles and yielded systematic increase in lateral expansion rates of biofilms when grown on an agar surface. At the microscopic level, we observed an intriguing NaCl-induced transition from slow and isotropic super diffusion behavior of individual bacterial cells to rapid anisotropic diffusion behavior. The average orientation of the cells (at the edge) along the expanding direction and the increased flagellation in a subpopulation of cells in the presence of NaCl corroborates well with the observed rapid anisotropic diffusion of cells. Further, the cellular uptake of NaCl resulted in the downregulation of several genes underlying the formation of biofilms, revealing the role of chemical cues like NaCl in controlling the gene regulatory circuit underlying the sessile to motile transition. Our study opens a new avenue to decipher the competitive advantage provided to the subcellular populations by NaCl due to lifestyle switch in Bacillus subtilis.

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“…formation (Ryan-Payseur & Freitag, 2018;Zhang et al, 2020). A pdeB mutant is unable to form biofilms and inactivation of the gene leads to haemolysis in L. monocytogenes.…”

Section: Collecti Ve Behaviour Of Bac I Llus Su Bti Lis Cells: Swimmi...mentioning

confidence: 99%

A model industrial workhorse: Bacillus subtilis strain 168 and its genome after a quarter of a century

Bremer

Calteau

Danchin

et al. 2023

Microbial Biotechnology

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The vast majority of genomic sequences are automatically annotated using various software programs. The accuracy of these annotations depends heavily on the very few manual annotation efforts that combine verified experimental data with genomic sequences from model organisms. Here, we summarize the updated functional annotation of Bacillus subtilis strain 168, a quarter century after its genome sequence was first made public. Since the last such effort 5 years ago, 1168 genetic functions have been updated, allowing the construction of a new metabolic model of this organism of environmental and industrial interest. The emphasis in this review is on new metabolic insights, the role of metals in metabolism and macromolecule biosynthesis, functions involved in biofilm formation, features controlling cell growth, and finally, protein agents that allow class discrimination, thus allowing maintenance management, and accuracy of all cell processes. New 'genomic objects' and an extensive updated literature review have been included for the sequence, now available at the International Nucleotide Sequence Database Collaboration (INSDC: AccNum AL009126.4).

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Bacillus subtilis Biofilms: a Matter of Individual Choice

Cited by 11 publications

References 7 publications

Periplaneta americana: Antibiotic susceptibility profiles of associated biofilm-forming bacteria

Periplaneta americana: Antibiotic susceptibility profiles of associated biofilm-forming bacteria

NaCl Triggers the Sessile-to-Motile Transition of Bacillus subtilis

A model industrial workhorse: Bacillus subtilis strain 168 and its genome after a quarter of a century

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