Babesia microtiin AdultDermacentor reticulatusTicks from Eastern Poland

Wójcik-Fatla, Angelina; Bartosik, Katarzyna; Buczek, Alicja; Dutkiewicz, Jacek

doi:10.1089/vbz.2011.0904

Cited by 34 publications

(29 citation statements)

References 22 publications

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“…Moreover, 5 rodents were solely positive in spleen and not in blood. According to our results and to those from former studies [Wójcik-Fatla et al 2012], I. ricinus may serve as a bridge vector [Silaghi et al 2012;Overzier et al 2013]. On the contrary, we cannot confirm that D. reticulatus ticks may serve as bridge vectors for B. microti even though its role has also been discussed before [Wójcik-Fatla et al 2012].…”

Section: Discussioncontrasting

confidence: 87%

“…According to our results and to those from former studies [Wójcik-Fatla et al 2012], I. ricinus may serve as a bridge vector [Silaghi et al 2012;Overzier et al 2013]. On the contrary, we cannot confirm that D. reticulatus ticks may serve as bridge vectors for B. microti even though its role has also been discussed before [Wójcik-Fatla et al 2012]. The sequences obtained from both ticks and rodents all showed a 100% identity to each other and to sequences deposited under following GenBank accession no.…”

Section: Discussioncontrasting

confidence: 74%

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Molecular examinations of Babesia microti in rodents and rodent-attached ticks from urban and sylvatic habitats in Germany

Obiegala

Pfeffer

Pfister

et al. 2015

Ticks and Tick-borne Diseases

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Small mammals serve as reservoir hosts for tick-borne pathogens, especially for those which are not transmitted transovarially in ticks -such as Babesia microti. Molecular investigations on the prevalence of B. microti in wild small mammals and on attached ticks from differently structured areas may provide information on the circulation of B. microti in different ecological niches. In 2012 and 2013, 622 rodents (396 Myodes glareolus, 178 Apodemus flavicollis, 36 Apodemus sylvaticus, 4 Apodemus agrarius, 7 Microtus arvalis, 1 Microtus agrestis) were captured from three differently structured habitats (urban, sylvatic, recultivated) in Germany. Attached ticks were collected from 449 small mammals (3250 Ixodes ricinus, 7 Ixodes trianguliceps, 133 Dermacentor reticulatus). A representative selection of a maximum of 5 ticks per developmental stage and species per 30 rodents of each species, location and year resulting in 965 ticks was further investigated. DNA was extracted from tick, blood and spleen samples, and tested by PCR for the partial 18S rRNA gene of B. microti with subsequent sequencing. The prevalence was significantly higher in rodents from the sylvatic site (4.6%) than in rodents captured at both other sites (0-0.6%) (2= 11.95; p= 0.00125). Body and spleen weight of infected M. glareolus from the sylvatic site were significantly higher compared to those from non-infected individuals from that site (p = 0.00288 and p = 0.00017, respectively). Babesia microti DNA was detected in 3 out of 965 attached ticks (0.3%; 95%CI: 0-1) from all sites, but they derived exclusively from rodents captured at the sylvatic site. At the same site, I. ricinus nymphs (7.7%; 95%CI: 1-25.3) were significantly more often infected than I. ricinus larvae (0%; 95%CI: 0-1.3)(2 = 26.72; p <0.0001). The majority of positive rodents was also found at that site. I. trianguliceps occurred exclusively and the majority of M. glareolus at that site. Thus, it may be assumed that the circulation of B. microti is more efficient where this tick species and voles exist sympatrically than in areas with a predominant occurrence of Apodemus species. microti with subsequent sequencing. The prevalence was significantly higher in rodents from the sylvatic site (4.6%) than in rodents captured at both other sites (0-0.6%) (χ²= 11.95; p= 0.00125).Body and spleen weight of infected M. glareolus from the sylvatic site were significantly higher compared to those from non-infected individuals from that site (p=0.00288 and p=0.00017, respectively). Babesia microti DNA was detected in 3 out of 965 attached ticks (0.3%; 95%CI: 0-1) from all sites, but they derived exclusively from rodents captured at the sylvatic site. At the same site, I. ricinus nymphs (7.7%; 95%CI: 1-25.3) were significantly more often infected than I. ricinus larvae (0%; 95%CI: 0-1.3)(χ²=26.72; p <0.0001). The majority of positive rodents was also found at that site. I. trianguliceps occurred exclusively and the majority of M. glareolus at that site. Thus, it may be assumed that...

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Section: Discussioncontrasting

confidence: 87%

Section: Discussioncontrasting

confidence: 74%

Molecular examinations of Babesia microti in rodents and rodent-attached ticks from urban and sylvatic habitats in Germany

Obiegala

Pfeffer

Pfister

et al. 2015

Ticks and Tick-borne Diseases

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“…Rhipicephalus rossicus are reported (3,4,12,16,18). I. ricinus is the most prevalent tick species in Poland, which can transmit Borrelia burgdorferi sensu lato and Anaplasma phagocytophilum, the aetiological agents of Lyme disease and granulocytic anaplasmosis (7).…”

Section: Frequently I Apronophorus I Trianguliceps I Hexagonusmentioning

confidence: 99%

Prevalence of Babesia canis, Borrelia burgdorferi sensu lato, and Anaplasma phagocytophilum in hard ticks collected from meadows of Lubelskie Voivodship (eastern Poland)

Dzięgiel

Kubrak

Adaszek

et al. 2014

Bulletin of the Veterinary Institute in Pulawy

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The aim of the study was to assess the distribution of Anaplasma phagocytophilum, Borrelia burgdorferi sensu lato, and Babesia canis in adult females and males of Ixodes ricinus and Dermacentor reticulatus ticks, inhabiting meadows near large forest complexes throughout the Lubelskie Voivodship (eastern region of Poland). Ticks were collected using the flagging method. Among 720 ticks collected, 506 were identified as D. reticulatus, and 214 as I. ricinus. DNA of B. canis and B. burgdorferi s.l. was detected in 21.3% and 0.6% of D. reticulatus ticks, respectively. In I. ricinus ticks, DNA specific to B. burgdorferi s.l. and A. phagocytophilum was detected in 5.6% and 10.3%, respectively. Co-infections of B. burgdorferi s.l. and A. phagocytophilum were found in two I. ricinus ticks. These results indicate that the Lublin region is an area at risk of tick-borne diseases of humans and animals, which must be considered in clinical practice.

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“…Nevertheless, D. reticulatus has been demonstrated to harbour various tick-borne pathogens of humans and/or animals, such as tick-borne encephalitis virus (TBEV), Omsk haemorrhagic fever virus, Kemerovo virus, Anaplasma phagocytophilum, Anaplasma marginale, Spotted Fever Group Rickettsiae (SFGR), such as Rickettsia raoultii and Rickettsia slovaca, Borrelia burgdorferi sensu lato (s.l. ), Francisella tularensis and Francisella-like endosymbionts, Coxiella burnetii, Bartonella spp., Babesia canis, Babesia caballi, Babesia microti, Babesia divergens, Babesia bigemina, and Theileria equi [7,8,[18][19][20][21][22][23][24][25][26][27][28][29][30][31][32].…”

Section: Introductionmentioning

confidence: 99%

“…The role of this tick species in the transmission of some other pathogens in domestic animals and humans was also confirmed for Babesia caballi, Theileria equi, Anaplasma marginale, and Omsk haemorrhagic fever virus [18]. The role of D. reticulatus as a vector of other pathogens is less known, but seems probable with relevance to TBEV, and less probable with relevance to Francisella tularensis, Coxiella burnetii and Babesia microti [8,18,24,25,[29][30][31]35].…”

Section: Introductionmentioning

confidence: 99%

Prevalence of infections and co-infections with 6 pathogens in Dermacentor reticulatus ticks collected in eastern Poland

Zając

Wójcik-Fatla

Sawczyn

et al. 2017

Ann Agric Environ Med.

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Occurrence of co-infections with various pathogens in ixodid ticks creates a risk of increased severity of tick-borne diseases in humans and animals exposed to bite of the ticks carrying multiple pathogens. Accordingly, co-infections in ticks were subject of numerous analyses, but almost exclusively with regard to Ixodes ricinus complex whereas potential tick vectors belonging to other genera were much less studied. Taking into consideration the role of Dermacentor reticulatus in the transmission of various pathogens, we carried out for the first time the comprehensive statistical analysis of co-infections occurring in this tick species. An attempt was made to determine the significance of the associations between 6 different pathogens occurring in D. reticulatus (Tick-borne encephalitis virus = TBEV, Anaplasma phagocytophilum, Rickettsia raoultii, Borrelia burgdorferi s. l., Babesia spp., Toxoplasma gondii), using 2 statistical methods: determination of Odds Ratios (ORs) and the Fisher's exact test. 634 questing Dermacentor reticulatus ticks (370 females and 264 males) were collected in 2011-2013 by flagging the lower vegetation in 3 localities in the area of Łęczyńsko-Włodawskie Lakeland, situated in the Lublin region of eastern Poland. The presence of individual pathogens was detected by PCR. Ticks were infected most often with Rickettsia raoultii (43.8%), less with TBEV (8.5%), and much less with Babesia spp., Toxoplasma gondii, Borrelia burgdorferi s.l., and Anaplasma phagocytophilum (2.5%, 2.1%, 1.6% and 1.1%, respectively). The locality-dependent variability proved to be significant for TBEV (c 2 =11.063; P=0.004) and Toxoplasma gondii (c 2 =11.298; P=0.0035), but not for other pathogens. Two hundred seventy (42.6%) of the examined ticks were infected only with a single pathogen, and 54 (8.5%) showed the presence of dual co-infections, each with 2 pathogens. The most common were dual infections with participation of Rickettsia raoultii (7.41%); next, those with participation of the TBEV (5.21%), Toxoplasma gondii (1.58%), Borrelia burgdorferi s.l. (1.26%), Anaplasma phagocytophilum (0.95%), and Babesia spp. (0.63%). On the total number of 15 possible associations, in 9 cases co-infections occurred whereas in 6 cases they were not detected. The most noteworthy were positive co-infections with the participation of TBEV, which proved to be weakly significant (0.05

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Babesia microtiin AdultDermacentor reticulatusTicks from Eastern Poland

Cited by 34 publications

References 22 publications

Molecular examinations of Babesia microti in rodents and rodent-attached ticks from urban and sylvatic habitats in Germany

Molecular examinations of Babesia microti in rodents and rodent-attached ticks from urban and sylvatic habitats in Germany

Prevalence of Babesia canis, Borrelia burgdorferi sensu lato, and Anaplasma phagocytophilum in hard ticks collected from meadows of Lubelskie Voivodship (eastern Poland)

Prevalence of infections and co-infections with 6 pathogens in <i>Dermacentor reticulatus</i> ticks collected in eastern Poland

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