Azospirillum brasilenseinoculation counteracts the induction of nitrate uptake in maize plants

Nutraceutical Profiles of Two Hydroponically Grown Sweet Basil Cultivars as Affected by the Composition of the Nutrient Solution and the Inoculation With Azospirillum brasilense

“…Consistently, as previously observed (Nikolic et al, 2007), NO 3 − provision is a fundamental prerequisite for an efficient reduction and uptake of Fe in dicot plants, thus leading to an increased microelement concentration in the leaves of both basil cultivars. Yet, the inoculation with A. brasilense, which was shown to be effective in enhancing the Fe content in cucumber and maize plants (Pii et al, 2015c(Pii et al, , 2016(Pii et al, , 2019, did not display any influence in the Fe uptake and allocation in basil plants, further underlining the species-specific response of plants to the inoculation with the PGPR. On the other hand, the SO 4 2− fertilization and the inoculation with A. brasilense caused the increase in the concentration of Cu in cv.…”

“…Red Rubin . Up to now, Azospirillum inoculation has been shown to induce various effects on plant physiological parameters; in fact, it has been demonstrated to increase plant growth parameters in strawberry ( Guerrero-Molina et al, 2014 ; Pii et al, 2018 ) and corn ( Zaady et al, 1993 ; Pii et al, 2019 ). Nonetheless, it has also been demonstrated that the growth-promoting effects of A. brasilense are strongly dependent on plant species and genotypes ( Pedraza et al, 2010 ; Pii et al, 2018 ).…”

“…brasilense Cd (DSM-1843) was grown in solid Luria-Bertani (LB) growth medium (10 g L −1 tryptone, 5 g L −1 yeast extract, 10 g L −1 NaCl, 14 g L −1 agar) for 3 days at 28 • C. Afterward, the microbial biomass was inoculated in liquid LB medium and grown at 28 • C under horizontal shaking until saturation. Bacteria were collected by centrifugation for 15 min at 4,500 rpm and washed once with sterile deionized water, as previously described (Pii et al, 2019). The concentration of bacteria was estimated by a spectrophotometer at 600 nm optical density.…”

“…To this aim, basil plants were hydroponically grown with two nutrient solutions, fortified for the concentration of either S, supplied as SO 4 2– , or N, supplied as NO 3 – . In addition, plants were inoculated with A. brasilense , which is already known (i) to influence the molecular and biochemical mechanisms underlying the nutrients acquisition in model plants ( Pii et al, 2015c , 2016 , 2019 ; Marastoni et al, 2019 ) and (ii) determine alteration in both quantitative and qualitative traits of strawberry fruits ( Pii et al, 2018 ). The effects of cultivation practices (i.e., nutrient solution fortification and inoculation with beneficial microbes) on the nutraceutical composition in basil leaves have been assessed by the application of a holistic approach, combining metabolomics and ionomic analyses, together with traditional analytical methods, based on both spectrophotometric and high-performance liquid chromatography (HPLC) analyses.…”

Recent Achievements and New Research Opportunities for Optimizing Macronutrient Availability, Acquisition, and Distribution for Perennial Fruit Crops

“…On the other side, LATS is not saturable and responses are linearly related to external nitrate concentrations, provided that it is higher than 0.5-1 mM [115]. Interestingly, recent pieces of evidence demonstrated that the inoculation of crop plants (i.e., maize) with N-fixing rhizobacteria can alter the physiology of nitrate uptake at the root level, highlighting that the concomitant application of conventional fertilizers and bioinoculants could not necessarily result in an enhancement in the nutrient uptake/use efficiency, particularly when nitrate is available at low concentrations at the rhizosphere [116].…”