<i>AXR3</i>and<i>SHY2</i>interact to regulate root hair development

Knox, Kirsten; Grierson, Claire; Leyser, Ottoline

doi:10.1242/dev.00659

Cited by 160 publications

(143 citation statements)

References 42 publications

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“…Phenotypic analysis of the mutants reveals distinct and overlapping functions among the various members. For example, expression of the stabilized mutant form of iaa3/shy2 or iaa17/axr3 proteins from a heat shock promoter causes different effects on root hair development (Knox et al, 2003). The phenotype of the gain-of-function mutants can be mimicked by the expression of corresponding Aux/IAA proteins with the domain II mutation under the control of their own promoters (Fukaki et al, 2002(Fukaki et al, , 2005Weijers et al, 2005).…”

Section: Discussion Functional Redundancy Among the Aux/iaa Gene Famimentioning

confidence: 99%

Functional Genomic Analysis of theAUXIN/INDOLE-3-ACETIC ACIDGene Family Members inArabidopsis thaliana [W]

et al. 2005

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Auxin regulates various aspects of plant growth and development. The AUXIN/INDOLE-3-ACETIC ACID (Aux/IAA) genes encode short-lived transcriptional repressors that are targeted by the TRANSPORT INHIBITOR RESPONSE1/AUXIN RECEPTOR F-BOX proteins. The Aux/IAA proteins regulate auxin-mediated gene expression by interacting with members of the AUXIN RESPONSE FACTOR protein family. Aux/IAA function is poorly understood; herein, we report the identification and characterization of insertion mutants in 12 of the 29 Aux/IAA family members. The mutants show no visible developmental defects compared with the wild type. Double or triple mutants of closely related Aux/IAA genes, such as iaa8-1 iaa9-1 or iaa5-1 iaa6-1 iaa19-1, also exhibit wild-type phenotypes. Global gene expression analysis reveals that the molecular phenotypes of auxin-treated and untreated light-grown seedlings are unaffected in the iaa17-6 and iaa5-1 iaa6-1 iaa19-1 mutants. By contrast, similar analysis with the gain-of-function axr3-1/iaa17-1 mutant seedlings reveals dramatic changes in basal and auxin-induced gene expression compared with the wild type. Expression of several type-A ARABIDOPSIS RESPONSE REGULATOR genes and a number of genes involved in cell wall biosynthesis and degradation is repressed in axr3-1/iaa17-1. The data suggest extensive functional redundancy among Aux/IAA gene family members and that enhanced stability of the AXR3/IAA17 protein severely alters the molecular phenotype, resulting in developmental defects.

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Section: Discussion Functional Redundancy Among the Aux/iaa Gene Famimentioning

confidence: 99%

Functional Genomic Analysis of theAUXIN/INDOLE-3-ACETIC ACIDGene Family Members inArabidopsis thaliana [W]

et al. 2005

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“…It has been shown that AUX1 promoted root hair initiation by stimulating auxin influx to specific sites (Rahman et al, 2002;Jones et al, 2009), and overexpression of AUX1 or PIN2 was found to promote or block the root hair elongation (Cho et al, 2007;Ganguly et al, 2010). In addition to regulating the auxin transporter genes, auxin also induces the expression of genes in the AUXIN/INDOLE-3-ACETIC ACID (Aux/IAA) family, including auxin response gene IAA3, which plays important roles in auxin-mediated regulation of root growth, gravitropism, and lateral root formation (Tian and Reed, 1999;Tian et al, 2002;Knox et al, 2003).…”

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confidence: 99%

The Cotton Transcription Factor TCP14 Functions in Auxin-Mediated Epidermal Cell Differentiation and Elongation

Wang

Zhao²,

Cheng³

et al. 2013

Plant Physiology

111

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Plant-specific TEOSINTE-BRANCHED1/CYCLOIDEA/PCF (TCP) transcription factors play crucial roles in development, but their functional mechanisms remain largely unknown. Here, we characterized the cellular functions of the class I TCP transcription factor GhTCP14 from upland cotton (Gossypium hirsutum). GhTCP14 is expressed predominantly in fiber cells, especially at the initiation and elongation stages of development, and its expression increased in response to exogenous auxin. Induced heterologous overexpression of GhTCP14 in Arabidopsis (Arabidopsis thaliana) enhanced initiation and elongation of trichomes and root hairs. In addition, root gravitropism was severely affected, similar to mutant of the auxin efflux carrier PIN-FORMED2 (PIN2) gene. Examination of auxin distribution in GhTCP14-expressing Arabidopsis by observation of auxin-responsive reporters revealed substantial alterations in auxin distribution in sepal trichomes and root cortical regions. Consistent with these changes, expression of the auxin uptake carrier AUXIN1 (AUX1) was up-regulated and PIN2 expression was down-regulated in the GhTCP14-expressing plants. The association of GhTCP14 with auxin responses was also evidenced by the enhanced expression of auxin response gene IAA3, a gene in the AUXIN/INDOLE-3-ACETIC ACID (Aux/IAA) family. Electrophoretic mobility shift assays showed that GhTCP14 bound the promoters of PIN2, IAA3, and AUX1, and transactivation assays indicated that GhTCP14 had transcription activation activity. Taken together, these results demonstrate that GhTCP14 is a dual-function transcription factor able to positively or negatively regulate expression of auxin response and transporter genes, thus potentially acting as a crucial regulator in auxin-mediated differentiation and elongation of cotton fiber cells.

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“…Although there are very few phenotypes associated with loss of Aux/IAA function , gain-of-function mutations in different Aux/IAAs give rise to a range of striking phenotypes, some common and some entirely opposite (Liscum and Reed 2002). It is the latter group that are informative here because although some differences can be attributed to the expression pattern of the stabilized proteins, several of these opposite phenotypes persist when the mutant proteins are expressed in the same cell type and/or from the same promoter (Knox et al 2003;Weijers et al 2005a;Muto et al 2007). A good example is the effect of stabilized SHY2/IAA3 and AXR3/IAA17 proteins on the production of root hairs.…”

Section: Specific Interactions In the Afb-aux/iaa-arf Systemmentioning

confidence: 98%

“…A good example is the effect of stabilized SHY2/IAA3 and AXR3/IAA17 proteins on the production of root hairs. Driven by the same inducible promoter, too much IAA3 protein results in longer root hairs whereas too much IAA17 protein stops root hair production all together (Knox et al 2003). Whatever the details of the molecular basis of these phenotypes, it is clear that these mutant proteins can do different things and although there are other possibilities, the most parsimonious explanation is that they are interacting predominantly with different ARFs, which are in turn targeting distinct subsets of auxin-regulated loci.…”

Section: Specific Interactions In the Afb-aux/iaa-arf Systemmentioning

confidence: 99%

Context, Specificity, and Self-Organization in Auxin Response

Bianco

Kepinski

2010

Cold Spring Harbor Perspectives in Biology

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Auxin is a simple molecule with a remarkable ability to control plant growth, differentiation, and morphogenesis. The mechanistic basis for this versatility appears to stem from the highly complex nature of the networks regulating auxin metabolism, transport and response. These heavily feedback-regulated and inter-dependent mechanisms are complicated in structure and complex in operation giving rise to a system with self-organizing properties capable of generating highly context-specific responses to auxin as a single, generic signal.

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AXR3andSHY2interact to regulate root hair development

Cited by 160 publications

References 42 publications

Functional Genomic Analysis of theAUXIN/INDOLE-3-ACETIC ACIDGene Family Members inArabidopsis thaliana [W]

Functional Genomic Analysis of theAUXIN/INDOLE-3-ACETIC ACIDGene Family Members inArabidopsis thaliana [W]

The Cotton Transcription Factor TCP14 Functions in Auxin-Mediated Epidermal Cell Differentiation and Elongation

Context, Specificity, and Self-Organization in Auxin Response

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