<i>AtNUF2</i> modulates spindle microtubule organization and chromosome segregation during mitosis

Li, Jin; Wang, Yutao; Zou, Wenxuan; Jian, Liufang; Fu, Ying; Jie, Zhao

doi:10.1111/tpj.15347

Cited by 5 publications

(3 citation statements)

References 72 publications

(95 reference statements)

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“…These were transcripts encoding the kinetochore protein Nuf2 and kinesins. Alteration of Nuf2 in A. thaliana affects the microtubule organization and results in lagging chromatids (Li et al 2021). Interestingly, B-specific Nuf2 transcripts are also associated with the process of programmed elimination of B chromosomes in Aegilops speltoides embryos (Boudichevskaia et al 2020).…”

Section: Discussionmentioning

confidence: 99%

Rye B chromosomes differently influence the expression of A chromosome–encoded genes depending on the host species

et al. 2022

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The B chromosome (B) is a dispensable component of the genome in many species. To evaluate the impact of Bs on the transcriptome of the standard A chromosomes (A), comparative RNA-seq analyses of rye and wheat anthers with and without additional rye Bs were conducted. In both species, 5–6% of the A-derived transcripts across the entire genomes were differentially expressed in the presence of 2Bs. The GO term enrichment analysis revealed that Bs influence A chromosome encoded processes like “gene silencing”; “DNA methylation or demethylation”; “chromatin silencing”; “negative regulation of gene expression, epigenetic”; “post-embryonic development”; and “chromosome organization.” 244 B chromosome responsive A-located genes in + 2B rye and + B wheat shared the same biological function. Positively correlated with the number of Bs, 939 and 1391 B-specific transcripts were identified in + 2B and + 4B wheat samples, respectively. 85% of B-transcripts in + 2B were also found in + 4B transcriptomes. 297 B-specific transcripts were identified in + 2B rye, and 27% were common to the B-derived transcripts identified in + B wheat. Bs encode mobile elements and housekeeping genes, but most B-transcripts were without detectable similarity to known genes. Some of these genes are involved in cell division-related functions like Nuf2 and might indicate their importance in maintaining Bs. The transcriptome analysis provides new insights into the complex interrelationship between standard A chromosomes and supernumerary B chromosomes.

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Section: Discussionmentioning

confidence: 99%

Rye B chromosomes differently influence the expression of A chromosome–encoded genes depending on the host species

et al. 2022

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“…The DSP CDS was amplified and subsequently cloned into modified pET28a‐MBP vector (The MBP protein had been cloned into the downstream of the lac operator). The fused protein MBP–DSP was purified by Dextrin Beads 6FF (Smart‐Lifesciences, China) and MBP–AtNUF2 was used as the control protein (Li et al ., 2021b ). The biotin‐labelled probes were synthesized or amplified by PCR and labelled by using EMSA Probe Biotin Labelling Kit (Beyotime, China).…”

Section: Methodsmentioning

confidence: 99%

Rice DSP controls stigma, panicle and tiller primordium initiation

Yu,

Yao,

Mao

et al. 2023

Plant Biotechnology Journal

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SummaryTiller and seed number are key determinants of rice (Oryza sativa) yield. These traits are mainly affected by tiller, panicle, spikelet and stigma formation, but to date, no single gene involved in the development of all these organs has been identified. Here, we found a rice mutant defective stigma and panicle (dsp) with greatly reduced numbers of tillers and panicle branches, and ovaries lacking stigmas, due to defects in primordium initiation. We cloned DSP using sequencing‐based mapping and verified its function with the CRISPR/Cas9 system. DSP encodes a transcription factor containing an APETALA2/ETHYLENE RESPONSE FACTOR (AP2/ERF) domain that recognizes the GCC motif and a transcription‐activating domain at the site of 244–314 that contains an angiosperm‐related (AR) motif. Mutating the AR motif resulted in the dsp mutant phenotypes, whereas mutating the AP2/ERF domain led to seedling death. DSP directly regulated PINOID (PID) expression to determine the emergence of rice stigmas, and PID overexpression partially rescued the stigma defect in the dsp cr2‐8 and dsp mutants. Moreover, DSP indirectly affected LAX PANICLE1 (LAX1) expression to determine tiller primordium formation and synergistically regulated panicle primordium development. Our results indicated that DSP was a key regulator that modulated different genetic pathways to control the initiation of stigma primordia, the axillary meristem formation of tillers and panicle branches, which revealed their molecular mechanisms and cross‐networks, laying the vital foundation for rice yield and trait improvement.

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“…Kinetochore proteins have a great diversity in eukaryotes, and gene duplications played a major role in shaping differing kinetochore architectures (Tromer et al., 2019 ; van Hooff et al., 2017 ). The homologues of the KMN network have been identified in plants including Ndc80, Nuf2, Spc24, Knl1, Mis12 and Nnf1 (Allipra et al., 2021 ; Du and Dawe, 2007 ; Li et al., 2021 ; Li and Dawe, 2009 ; Shin et al., 2018 ; Su et al., 2021 ). They display conserved functions involved in microtubule organization and chromosome segregation through cell division, and the mutations cause defective developmental phenotypes in plants.…”

Section: Introductionmentioning

confidence: 99%

Centromeres: From chromosome biology to biotechnology applications and synthetic genomes in plants

Zhou

Liu

Guo

et al. 2022

Plant Biotechnology Journal

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Centromeres are the genomic regions that organize and regulate chromosome behaviours during cell cycle, and their variations are associated with genome instability, karyotype evolution and speciation in eukaryotes. The highly repetitive and epigenetic nature of centromeres were documented during the past half century. With the aid of rapid expansion in genomic biotechnology tools, the complete sequence and structural organization of several plant and human centromeres were revealed recently. Here, we systematically summarize the current knowledge of centromere biology with regard to the DNA compositions and the histone H3 variant (CENH3)-dependent centromere establishment and identity. We discuss the roles of centromere to ensure cell division and to maintain the three-dimensional (3D) genomic architecture in different species. We further highlight the potential applications of manipulating centromeres to generate haploids or to induce polyploids offspring in plant for breeding programs, and of targeting centromeres with CRISPR/Cas for chromosome engineering and speciation. Finally, we also assess the challenges and strategies for de novo design and synthesis of centromeres in plant artificial chromosomes. The biotechnology applications of plant centromeres will be of great potential for the genetic improvement of crops and precise synthetic breeding in the future.

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AtNUF2 modulates spindle microtubule organization and chromosome segregation during mitosis

Cited by 5 publications

References 72 publications

Rye B chromosomes differently influence the expression of A chromosome–encoded genes depending on the host species

Rye B chromosomes differently influence the expression of A chromosome–encoded genes depending on the host species

Rice DSP controls stigma, panicle and tiller primordium initiation

Centromeres: From chromosome biology to biotechnology applications and synthetic genomes in plants

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