<i>Astilbe thunbergii</i> reduces postprandial hyperglycemia in a type 2 diabetes rat model via pancreatic alpha-amylase inhibition by highly condensed procyanidins

Kato, Eisuke; Kushibiki, Natsuka; Inagaki, Yoshishige; Kurokawa, Mihoko; Kawabata, Jun

doi:10.1080/09168451.2017.1353403

Cited by 17 publications

(13 citation statements)

References 22 publications

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“…However, in some cases, high levels of blood glucose due to excess activity of alpha-amylase results in hyperglycemia [15]. Thus, the inhibition of alpha-amylase activity can reduce postprandial hyperglycemia and prevent the risk of diabetes development [16,17]. In this study, ethanol extract of M. indica leaves (MIL) was investigated for its capability against alpha-amylase activity.…”

Section: Alpha-amylase Inhibitory Activitymentioning

confidence: 99%

Mechanism of Action of Mangifera indica Leaves for Anti-Diabetic Activity

Ngo

2019

Sci. Pharm.

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Diabetes is a major metabolic disorder whose prevalence is increasing daily. Medicinal plants have played an important role in the prevention and treatment of type 2 diabetes via prophylactic and therapeutic management. In this study, Mangifera Indica leaf (MIL) extract was investigated for its promising anti-diabetic activity via an in vitro model. It was found that MIL extract possessed significant inhibition on alpha-amylase activity up to (51.4 ± 2.7)% at a concentration of 200 µg/mL. Moreover, glucose adsorption capacity of MIL was identified at (2.7 ± 0.19) mM glucose/g extract. Furthermore, the extract caused a significant increase in glucose uptake up to (143 ± 9.3)% in LO-2 liver cells. Notably, MIL extract was effective in scavenging (63.3 ± 2.1)% 1,1-diphenyl-2-picryl-hydrazyl (DPPH) and (71.6 ± 4.3)% 2,2-azinobis-3-ethyl benzothiazoline-6-sulfonic acid (ABTS)+ radicals and inhibiting (66 ± 4.9)% NO production from RAW264.7 cells without any cytotoxicity effects. Accordingly, M. indica leaves are suggested as a promising material for development of hypoglycemic products.

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Section: Alpha-amylase Inhibitory Activitymentioning

confidence: 99%

Mechanism of Action of Mangifera indica Leaves for Anti-Diabetic Activity

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2019

Sci. Pharm.

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“…Notably, α-amylase enzyme plays an important role in carbohydrate digestion and postprandial glucose increase in diabetic patients. Thus, the inhibition of α-amylase activity can reduce postprandial hyperglycemia and prevent the risk of diabetes [28,29]. Recently, wild bitter melon has been emerged as an alternative medicine for prevention and/or treatment of type 2 diabetes.…”

Section: Resultsmentioning

confidence: 99%

Investigation of Biological Activities of Wild Bitter Melon (Momordica charantia Linn. Var. Abbreviata Ser.)

Pham

Ngo

2019

Biomolecules

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Wild bitter melon (Momordica charantia L. var. Abbreviata Ser.) is a wild edible variety of M. charantia, often used in folk medicine. In this study, the biological activities of its extract and fractions were investigated in vitro. It was found that ethyl acetate (EA) fraction exhibited high 1,1-diphenyl-2-picryl-hydrazyl (DPPH) scavenging activity with a half maximal inhibitory concentration (IC50) value of 0.43 ± 0.04 mg/mL, while the chloroform (CF), EA, and n-butanol (Bu) fractions had strong 2,2-azinobis-3-ethyl benzothiazoline-6-sulfonic acid (ABTS)+ scavenging ability with IC50 values of 0.36 ± 0.04 mg/mL, 0.35 ± 0.02 mg/mL, and 0.35 ± 0.05 mg/mL, respectively. Moreover, the EA and Bu fractions exhibited the highest protective effect against H2O2-induced DNA damage in a concentration-dependent manner. Furthermore, the EA fraction was effective in the inhibition of enzyme α-amylase activity with an IC50 value of 0.27 ± 0.029 mg/mL. Finally, it was observed that the production of nitric oxide (NO), a pro-inflammatory mediator, was significantly reduced from LPS-stimulated murine macrophage RAW 264.7 cells by the ethanol extract (ET) and the EA fraction. Therefore, wild bitter melon could be considered as a promising biomaterial for the development of pharmaceutical products.

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“…Alpha-amylase assay resulted in the inhibition of AERM activity at an IC 50 of 19.0 µg/mL, while acarbose inhibited amylase with an IC 50 of 5.2 µg/mL ( Figure 8 ). Tannins have been suggested as potent inhibitors of human salivary and porcine pancreatic α-amylases [ 34 , 35 ], suggesting that PAs from AERM could be implicated in this potent inhibitory activity. In addition, it has been reported that PAs of cinnamon water extract inhibited the amyloid formation of Human islet amyloid polypeptide (hIAPP) in a dose-dependent manner.…”

Section: Resultsmentioning

confidence: 99%

Modulatory Effect of Polyphenolic Compounds from the Mangrove Tree Rhizophora mangle L. on Non-Alcoholic Fatty Liver Disease and Insulin Resistance in High-Fat Diet Obese Mice

et al. 2018

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No scientific report proves the action of the phytochemicals from the mangrove tree Rhizophora mangle in the treatment of diabetes. The aim of this work is to evaluate the effects of the acetonic extract of R. mangle barks (AERM) on type 2 diabetes. The main chemical constituents of the extract were analyzed by high-performance liquid chromatography (HPLC) and flow injection analysis electrospray-iontrap mass spectrometry (FIA-ESI-IT-MS/MS). High-fat diet (HFD)-fed mice were used as model of type 2 diabetes associated with obesity. After 4 weeks of AERM 5 or 50 mg/kg/day orally, glucose homeostasis was evaluated by insulin tolerance test (kiTT). Hepatic steatosis, triglycerides and gene expression were also evaluated. AERM consists of catechin, quercetin and chlorogenic acids derivatives. These metabolites have nutritional importance, obese mice treated with AERM (50 mg/kg) presented improvements in insulin resistance resulting in hepatic steatosis reductions associated with a strong inhibition of hepatic mRNA levels of CD36. The beneficial effects of AERM in an obesity model could be associated with its inhibitory α-amylase activity detected in vitro. Rhizophora mangle partially reverses insulin resistance and hepatic steatosis associated with obesity, supporting previous claims in traditional knowledge.

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Astilbe thunbergii reduces postprandial hyperglycemia in a type 2 diabetes rat model via pancreatic alpha-amylase inhibition by highly condensed procyanidins

Cited by 17 publications

References 22 publications

Mechanism of Action of Mangifera indica Leaves for Anti-Diabetic Activity

Mechanism of Action of Mangifera indica Leaves for Anti-Diabetic Activity

Investigation of Biological Activities of Wild Bitter Melon (Momordica charantia Linn. Var. Abbreviata Ser.)

Modulatory Effect of Polyphenolic Compounds from the Mangrove Tree Rhizophora mangle L. on Non-Alcoholic Fatty Liver Disease and Insulin Resistance in High-Fat Diet Obese Mice

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