<i>Asterionellopsis tropicalis</i>(Bacillariophyceae): a new tropical species found in diatom accumulations

Franco, Andréa de Oliveira da Rocha; They, Ng Haig; Canani, Luís Gustavo de Castro; Maggioni, Rodrigo; Odebrecht, Clarisse

doi:10.1111/jpy.12435

Cited by 17 publications

(9 citation statements)

References 33 publications

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“…showed the strongest similarity to the A. glacialis CCAP 1009/3 (FR865485) 18S rDNA gene in NCBI. A. glacialis sensu lato has been shown to possess a wide cryptic diversity that encompasses at least five separate species, A. glacialis , A. tropicalis , A. guyunusae, A. maritima , A. lenisilicea , and A. thurstonii , using ITS1, 5.8S rRNA, and ITS2 sequences ( Franco et al, 2016 ). To confirm that our isolates were indeed A. glacialis we further sequenced the ITS1, 5.8S rRNA, and ITS2 regions of all four Asterionellopsis isolates.…”

Section: Resultsmentioning

confidence: 99%

Bacterial Communities of Diatoms Display Strong Conservation Across Strains and Time

et al. 2018

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Interactions between phytoplankton and bacteria play important roles in shaping the microenvironment surrounding these organisms and in turn influence global biogeochemical cycles. This microenvironment, known as the phycosphere, is presumed to shape the bacterial diversity around phytoplankton and thus stimulate a diverse array of interactions between both groups. Although many studies have attempted to characterize bacterial communities that associate and interact with phytoplankton, bias in bacterial cultivation and consistency and persistence of bacterial communities across phytoplankton isolates likely impede the understanding of these microbial associations. Here, we isolate four strains of the diatom Asterionellopsis glacialis and three strains of the diatom Nitzschia longissima and show through metabarcoding of the bacterial 16S rDNA gene that though each species possesses a unique bacterial community, the bacterial composition across strains from the same species are highly conserved at the genus level. Cultivation of all seven strains in the laboratory for longer than 1 year resulted in only small changes to the bacterial composition, suggesting that despite strong pressures from laboratory culturing conditions associations between these diatoms and their bacterial communities are robust. Specific operational taxonomic units (OTUs) belonging to the Roseobacter-clade appear to be conserved across all strains and time, suggesting their importance to diatoms. In addition, we isolate a range of cultivable bacteria from one of these cultures, A. glacialis strain A3, including several strains of Shimia marina and Nautella sp. that appear closely related to OTUs conserved across all strains and times. Coculturing of A3 with some of its cultivable bacteria as well as other diatom-associated bacteria shows a wide range of responses that include enhancing diatom growth. Cumulatively, these findings suggest that phytoplankton possess unique microbiomes that are consistent across strains and temporal scales.

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Section: Resultsmentioning

confidence: 99%

Bacterial Communities of Diatoms Display Strong Conservation Across Strains and Time

et al. 2018

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“…The diatom A. glacialis is a ubiquitous species that has been isolated from almost every major water body around the world (Korner, 1970; Kaczmarska et al ., 2014) and has recently been shown to be one of several abundant and widely distributed groups of diatoms in the open ocean from the Tara Oceans expedition (Malviya et al ., 2016). In addition, A. glacialis often forms blooms and dense patches worldwide (Karentz and Smayda, 1984; Franco et al ., 2016) that are characterized by high DOM secretions (Abreu et al ., 2003), making this diatom an ideal model system to examine interactions with bacteria. Here, we characterize AHL molecules produced by bacteria isolated from the phycosphere of A. glacialis and examine the influence of these AHLs on the ability of beneficial and opportunistic bacteria to colonize the phycosphere of A. glacialis .…”

Section: Introductionmentioning

confidence: 99%

Quorum sensing regulates ‘swim‐or‐stick’ lifestyle in the phycosphere

Cong

Ochsenkühn

Shibl

et al. 2020

Environmental Microbiology

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Summary Interactions between phytoplankton and bacteria play major roles in global biogeochemical cycles and oceanic nutrient fluxes. These interactions occur in the microenvironment surrounding phytoplankton cells, known as the phycosphere. Bacteria in the phycosphere use either chemotaxis or attachment to benefit from algal excretions. Both processes are regulated by quorum sensing (QS), a cell–cell signalling mechanism that uses small infochemicals to coordinate bacterial gene expression. However, the role of QS in regulating bacterial attachment in the phycosphere is not clear. Here, we isolated a Sulfitobacter pseudonitzschiae F5 and a Phaeobacter sp. F10 belonging to the marine Roseobacter group and an Alteromonas macleodii F12 belonging to Alteromonadaceae, from the microbial community of the ubiquitous diatom Asterionellopsis glacialis. We show that only the Roseobacter group isolates (diatom symbionts) can attach to diatom transparent exopolymeric particles. Despite all three bacteria possessing genes involved in motility, chemotaxis, and attachment, only S. pseudonitzschiae F5 and Phaeobacter sp. F10 possessed complete QS systems and could synthesize QS signals. Using UHPLC–MS/MS, we identified three QS molecules produced by both bacteria of which only 3‐oxo‐C16:1‐HSL strongly inhibited bacterial motility and stimulated attachment in the phycosphere. These findings suggest that QS signals enable colonization of the phycosphere by algal symbionts.

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“…Also, small flagellates (< 20 μm) and coccoid cells were grouped and counted in size classes. Molecular biology tools were applied to species of Asterionellopsis (Franco et al, 2016) and Pseudo-nitzschia (Hagström et al, 2011). Non-identifiable or damaged specimens were annotated as NA (not available).…”

Section: Phytoplankton (Dataset -I)mentioning

confidence: 99%

Patos Lagoon Estuary and Adjacent Marine Coastal Biodiversity Long-term data

Lemos

Lanari

Copertino

et al. 2021

Preprint

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Abstract. Estuaries are among the most productive aquatic ecosystems and provide important ecological and economic services in coastal areas. However, estuarine systems have been threatened worldwide by natural and anthropogenic impacts acting on local, regional and global scales. Long-term ecological studies contribute to the understanding and management of estuarine functioning, and provide the baseline information for detection changes and modeling of predictive scenarios. Here, we describe long-term data on the biodiversity and physico-chemical parameters obtained from 1993 to 2016 for the Patos Lagoon estuary and adjacent marine coast, in southern Brazil. We report eight datasets containing 6,972 sampling events with the occurrence and abundance records of 275 species (Kingdoms: Bacteria, Protozoa, Chromista, Plantae and Animalia) of functional groups plankton, benthos and nekton. Datasets also include 22,190 abiotic records. Database is published in the Global Biodiversity Information Facility (GBIF) repository (see Data availability in Sect 3). The present compendium represents one of the most comprehensive and longest datasets from primary producers to top predators in an estuarine-coastal system in South America and their availability will be an important contribution to the understanding and predictability of estuarine dynamics around the world.

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Asterionellopsis tropicalis(Bacillariophyceae): a new tropical species found in diatom accumulations

Cited by 17 publications

References 33 publications

Bacterial Communities of Diatoms Display Strong Conservation Across Strains and Time

Bacterial Communities of Diatoms Display Strong Conservation Across Strains and Time

Quorum sensing regulates ‘swim‐or‐stick’ lifestyle in the phycosphere

Patos Lagoon Estuary and Adjacent Marine Coastal Biodiversity Long-term data

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