<i>Aspergillus-Pseudomonas</i> interaction, relevant to competition in airways

Sass, Gabriele; Nazik, Hasan; Penner, John C.; Shah, Hemi; Ansari, Shajia Rahman; Clemons, Karl V.; Groleau, Marie‐Christine; Dietl, Anna-Maria; Visca, Paolo; Haas, Hubertus; Déziel, Éric; Stevens, David A.

doi:10.1093/mmy/myy087

Cited by 38 publications

(56 citation statements)

References 43 publications

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“…Hoffman et al (2009) suggested that, like mucoidy, the inactivation of lasR in Pa is associated with the progression of CF lung disease. The mutants lacking QS may contribute to the progression of lung disease by positively affecting the virulence of other microbes, as demonstrated for Ed by the current study and by other studies of A. fumigatus or C. albicans (De Sordi and Mühlschlegel, 2009;Sass et al, 2019).…”

Section: Discussionsupporting

confidence: 69%

“…AHL-QS molecules, particularly 3-oxo-C12 HSL, secreted by Pa are reported to influence the morphology of C. albicans, inhibiting the formation of C. albicans filaments, thereby impairing biofilm formation (Hogan et al, 2004;De Sordi and Mühlschlegel, 2009;Holcombe et al, 2010;Bandara et al, 2013;Bergeron et al, 2017). Furthermore, it is known that Pa interacts with A. fumigatus in inhibiting fungal growth and biofilm formation (Sass et al, 2019). A Pa phenotype and a source-dependent effect have been identified: CF isolates are more inhibitory than non-CF isolates, and non-mucoid isolates are more inhibitory than mucoid isolates (Ferreira et al, 2015).…”

Section: Discussionmentioning

confidence: 99%

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Phenotypical Characteristics of the Black Yeast Exophiala dermatitidis Are Affected by Pseudomonas aeruginosa in an Artificial Sputum Medium Mimicking Cystic Fibrosis–Like Conditions

et al. 2020

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Research into the cooperative pathogenicity of microbes in cystic fibrosis (CF) lungs is crucial for an understanding of the pathophysiology of infections and the development of novel treatment strategies. This study investigated the impact of the common CFassociated bacterial pathogen Pseudomonas aeruginosa on the black yeast Exophiala dermatitidis. It evaluated the planktonic growth, biofilm formation, morphology, and virulence of the fungus in the presence or absence of P. aeruginosa. It also determined the role of P. aeruginosa quorum-sensing (QS) molecules within these interactions, e.g., by using sterile culture filtrate and QS-deficient mutants. P. aeruginosa is known to inhibit the planktonic growth of E. dermatitidis. We found that fungal biofilm formation increased in the presence of P. aeruginosa after 24 h but is decreased significantly after 48 h. This effect was reversed when, instead of QS wild-type strains, lasR, and rhlR mutants were added to E. dermatitidis biofilm formation. The number and length of hyphae were substantially reduced when E. dermatitidis was co-cultivated with P. aeruginosa, but not when it was co-cultivated with the mutants. Experiments testing the virulence of E. dermatitidis in the greater wax moth Galleria mellonella showed a synergetic effect on larval killing when E. dermatitidis was injected together with P. aeruginosa culture filtrate. Survival rates were decreased when biofilm culture filtrate was added but not when planktonic culture filtrate was added. In summary, P. aeruginosa affects the growth, morphology, biofilm formation, and virulence of E. dermatitidis. N-acyl-L-homoserine lactone (AHL) QS molecules regulated factors that have been shown to contribute to the inhibition of the ability of E. dermatitidis to form filaments and biofilm.

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Section: Discussionsupporting

confidence: 69%

Section: Discussionmentioning

confidence: 99%

Phenotypical Characteristics of the Black Yeast Exophiala dermatitidis Are Affected by Pseudomonas aeruginosa in an Artificial Sputum Medium Mimicking Cystic Fibrosis–Like Conditions

et al. 2020

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“…26 Positive and negative interactions between Aspergillus and Pseudomonas aeruginosa, two central members of the fungal and bacterial pulmonary microbiota have also been reported. 26,27 Despite large and effective use of prophylaxis in acute myeloblastic leukemia (AML) patients and in allogeneic hematopoietic stem cell (alloHSCT) recipients, the overall incidence of IA continues to increase over time. [28][29][30] This increase can be explained by improved diagnosis, broader use of old and new immunosuppressive agents, and increase in organ transplantations.…”

Section: Epidemiologymentioning

confidence: 99%

Invasive Pulmonary Aspergillosis

Ledoux

Guffroy

Nivoix

et al. 2020

Semin Respir Crit Care Med

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Invasive pulmonary aspergillosis (IPA) remains difficult to diagnose and to treat. Most common risk factors are prolonged neutropenia, hematopoietic stem cell or solid organ transplantation, inherited or acquired immunodeficiency, administration of steroids or other immunosuppressive agents including monoclonal antibodies and new small molecules used for cancer therapy. Critically ill patients are also at high risk of IPA. Clinical signs are unspecific. Early computed tomography (CT)-scan identifies the two main aspects, angioinvasive and airway invasive aspergillosis. Although CT-scan findings are not fully specific they usually allow early initiation of therapy before mycological confirmation of the diagnosis. Role of 18F-fludeoxyglucose positron emission tomography with computed tomography (18F-FDG PET/CT) is discussed. Confirmation is based on microscopy and culture of respiratory samples, histopathology in case of biopsy, and importantly by detection of Aspergillus galactomannan using an immunoassay in serum and bronchoalveolar lavage fluid. Deoxyribonucleic acid detection by polymerase chain reaction is now standardized and increases the diagnosis yield. Two point of care tests detecting an Aspergillus glycoprotein using a lateral flow assay are also available. Mycological results allow classification into proven (irrespective of underlying condition), probable or possible (for cancer and severely immunosuppressed patients) or putative (for critically ill patients) IPA. New antifungal agents have been developed over the last 2 decades: new azoles (voriconazole, posaconazole, isavuconazole), lipid formulations of amphotericin B (liposomal amphotericin B, amphotericin B lipid complex), echinocandins (caspofungin, micafungin, anidulafungin). Results of main trials assessing these agents in monotherapy or in combination are presented as well as the recommendations for their use according to international guidelines. New agents are under development.

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“…Polymicrobial infections have increasingly become a focus of interest in infectious diseases research as inter-kingdom interplay of pathogens can mutually affect their virulence, susceptibility to antimicrobial therapy, and interactions with host immune surveillance [1]. In particular, Aspergillus fumigatus (AF) and Pseudomonas aeruginosa (PA) frequently co-colonize the airways of patients with cystic fibrosis, chronic bronchiectasis, or chronically immunocompromised patients and compete for nutrients in their ecological niche [2]. The mutually antagonistic relationship of AF and PA is, to a large extent, mediated by extracellular PA products, including toxins and siderophores [2].…”

Section: Introductionmentioning

confidence: 99%

“…Whereas most of these studies used conventional measurements such as growth inhibition or changes in metabolism [2], there is a need for robust and efficient experimental systems that specifically assess morphogenesis, a key feature of fungal virulence [5], in the context of bacterial interactions. Previous work revealed that dirhamnolipids released by PA induce ultrastructural modifications of AF hyphae, resulting in altered mycelial morphology and reduced growth of AF [6].…”

Section: Introductionmentioning

confidence: 99%

Live imaging and quantitative analysis of Aspergillus fumigatus growth and morphology during inter-microbial interaction with Pseudomonas aeruginosa

et al. 2020

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Pseudomonas aeruginosa (PA) and Aspergillus fumigatus (AF) chronically colonize the airways of patients with cystic fibrosis or chronic immunosuppression and mutually affect each other's pathogenesis. Here, we evaluated IncuCyte time-lapse imaging and NeuroTrack TM (NT) analysis (Wurster et al., 2019, mBio) as a toolbox to study mycelial expansion and morphogenesis of AF during interaction with PA. Co-incubation of AF with supernatant filtrates of wild-type (WT) PA strains strongly inhibited hyphal growth and branching. Consonant with prior metabolic studies, pyoverdine-deficient PA mutants had significantly attenuated inhibitory capacity. Accordingly, purified PA products pyoverdine and pyocyanin suppressed mycelial expansion of AF in a concentration-dependent way. Using fluorescence-guided tracking of GFP-AF293 mycelia during co-culture with live WT PA cells, we found significant inoculum-dependent mycelial growth inhibition and robust precision of the NT algorithm. Collectively, our experiments position IncuCyte NT as an efficient platform for longitudinal analysis of fungal growth and morphogenesis during bacterial co-infection.

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Aspergillus-Pseudomonas interaction, relevant to competition in airways

Cited by 38 publications

References 43 publications

Phenotypical Characteristics of the Black Yeast Exophiala dermatitidis Are Affected by Pseudomonas aeruginosa in an Artificial Sputum Medium Mimicking Cystic Fibrosis–Like Conditions

Phenotypical Characteristics of the Black Yeast Exophiala dermatitidis Are Affected by Pseudomonas aeruginosa in an Artificial Sputum Medium Mimicking Cystic Fibrosis–Like Conditions

Invasive Pulmonary Aspergillosis

Live imaging and quantitative analysis of Aspergillus fumigatus growth and morphology during inter-microbial interaction with Pseudomonas aeruginosa

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