<i>Aspergillus niger</i>, the causal agent of black mould disease in date fruits, infects and colonizes flowers and young fruitlets

Cohen, Yuval; Shulhani, R.; Rot, Yehuda; Zemach, Hanita; Belausov, Eduard; Grinberg-Baran, M.; Borenstein, M.; Pivonia, Shimon; Ezra, David; Shtienberg, D.

doi:10.1111/ppa.13358

Cited by 9 publications

(6 citation statements)

References 23 publications

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“…Compared to other fungal genera, Aspergillus species is the most prevalent pathogen and possesses a reputation for producing the most physiologically active secondary metabolites (Cairns et al, 2018;Cohen et al, 2021;Orfali et al, 2021).…”

Section: Discussionmentioning

confidence: 99%

Bioactive Secondary Metabolites from Aspergillus fumigatus ON428521 Isolated from Wadi El Rayan, El Fayum Governorate

Mokhtar

Nasr²,

Elaasser³

et al. 2022

Egypt. J. Bot.

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Egyptian Journal of Botany http://ejbo.journals.ekb.eg/ 15 F UNGI were isolated from Wadi El Rayan soils, and their secondary metabolites, extracted by petroleum ether, ethyl acetate, and chloroform, were tested for antimicrobial activities. Among the isolates, fungus-F1 extracts exhibited remarkable inhibitory activity against the tested microorganisms; therefore, it was subjected to morphological and molecular identification, and the fungus identified as Aspergillus fumigatus ON428521. With an inhibition zone ranging from 7mm to 21.4mm, ethyl acetate extract exerted the greatest activity. However, the petroleum ether extract only reached 16.5mm, and the chloroform extract demonstrated a lower activity level. Interestingly, activity was higher against tested Gram negative than those of Gram positive bacteria. Furthermore, Escherichia coli, Streptococcus mutans, and Cryptococcus neoformans were treated with ethyl acetate extract to explore possible effects on ultrastructure using transmission electron microscopy. The cell walls of treated cells lost their uniformity, ruptured, and became thicker. Moreover, the antitumor activities were evaluated against hepatocellular carcinoma (HepG2) and prostate carcinoma (PC-3) cell lines using the MTT assay, which revealed a high inhibitory activity with petroleum ether extract (IC 50 = 61.02 ± 1.64 and 90.31± 2.37µg/mL, respectively). Lower inhibition was detected with ethyl acetate extract (IC 50 of 84.42±3.36 and 121.98 ±3.46µg/ mL against HepG2 and PC-3, respectively). In addition, antioxidant activity was evaluated using the DPPH radical scavenging assay, and ethyl acetate extract demonstrated moderate activity with IC 50 = 278.24 ± 8.52µg/mL, followed by petroleum ether (581.07 ± 41.95µg/mL). The phytochemical analysis showed that the ethyl acetate extract is rich in phenolics, followed by flavonoids and alkaloids while lipids are the major component of the petroleum ether extract. GC-MS analysis of petroleum ether extract gives dodecanamine, N, N-dimethyl-, lupeol acetate, amyrin, kojic acid, and oleanenol-acetate as the major components. In conclusion, ethyl acetate and petroleum ether extracts of A. fumigatus F1 exhibited moderate antimicrobial, antioxidant, and antitumor properties.

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Section: Discussionmentioning

confidence: 99%

Bioactive Secondary Metabolites from Aspergillus fumigatus ON428521 Isolated from Wadi El Rayan, El Fayum Governorate

Mokhtar

Nasr²,

Elaasser³

et al. 2022

Egypt. J. Bot.

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“…In contrast to these beneficial aspects of Aspergillus spp., certain species like A.niger and A.flavus are reported as plant pathogens causing many plant diseases, for example, black rot in onions and black mould disease in date fruits caused by A.niger and fruit rot of peaches caused by A.flavus. (13,14) . Moreover A.flavus is well known for production of aflatoxins (15) .…”

Section: Antagonistic Activity Of the Extractmentioning

confidence: 99%

Exploration of Antagonistic Fungi From Rhizospheric Soil Against Phytopathogens of Solanum melongena and Citrus sinensis

Sabina,

Gayathridevi,

Pavithraa

2023

IJST

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Objectives: To explore the antagonistic activity of rhizospheric fungi against the phytopathogens of Solanum melongena (Brinjal) and Citrus sinensis (Orange), as these diseases cause major economic loss in the respective plants. Methods: Phytopathogens were isolated from infected leaves and fruits. Fungi were isolated from the rhizosphere of respective plants. Fungal isolates were screened for antagonism against phytopathogens and positive isolates were identified. Assays were conducted for volatile and non-volatile compounds from the antagonistic fungi and antagonistic metabolites were extracted. Antagonistic extracts were characterized and confirmed the antagonistic activity of the extract against the phytopathogens. Findings: Phytopathogens were identified as Curvularia sp. and Penicillium sp. Among the 12 rhizospheric fungal isolates, A. niger, A. fumigatus and A. flavus exhibited good antagonistic activity against the phytopathogens. The non-volatile metabolites were mostly responsible for antagonism when compared to the volatiles. The non-volatile compounds from A. niger showed highest activity against Curvularia (95% inhibition) followed by A. flavus (79%) and A. fumigatus (76%). This study demonstrates the potential applications of antagonistic fungi as effective biocontrol agents against various phytopathogens. Novelty: As high as 95% inhibition of phytopathogens was observed in the study which suggests the effectiveness of non-volatile metabolites from antagonistic fungi as potent biocontrol agents to prevent the crop-loss.

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“…Métodos. Se colectaron dátiles sintomáticos del ciclo de producción 2022, fueron lavados con agua estéril, se realizaron aislamientos en un medio de Papa-Dextrosa-Agar, se observaron macro y microscópicamente, y se comprobaron los postulados de Koch (Cohen et al, 2021). Los aislamientos de síntomas iniciales en pericarpio, mesocarpio, endocarpio y semilla de dátil se reinocularon en dátiles asintomáticos, a través de suspensión de esporas y con herida de aguja de disección estéril.…”

Section: Introductionunclassified

Aislamiento e identificación de hongos fitopatógenos en dátiles en poscosecha en Valle de Mexicali, México

Contreras Espinoza,

Valle Gough,

Núñez Ramírez

et al. 2023

Rev AIA

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Introducción. El cultivo de palma datilera (Phoenix dactylifera L.) es de gran relevancia económica en el noroeste de México, particularmente en el estado de Baja California. La superficie cultivada y el volumen de producción de dátiles se incrementó exponencialmente durante la última década; sin embargo, la presencia de enfermedades disminuye algunas características de calidad de los frutos, consideradas como defectos por normas alimentarias internacionales, siendo indispensable la identificación de agentes causales para su control. Objetivo. Aislar e identificar hongos fitopatógenos en dátiles en poscosecha en Valle de Mexicali. Métodos. Se colectaron dátiles sintomáticos del ciclo de producción 2022, fueron lavados con agua estéril, se realizaron aislamientos en un medio de Papa-Dextrosa-Agar, se observaron macro y microscópicamente, y se comprobaron los postulados de Koch (Cohen et al., 2021). Los aislamientos de síntomas iniciales en pericarpio, mesocarpio, endocarpio y semilla de dátil se reinocularon en dátiles asintomáticos, a través de suspensión de esporas y con herida de aguja de disección estéril. Se revisó el desarrollo de síntomas a los 3, 6, 9 y 12 días después de la inoculación, en los dátiles que mostraron el síntoma inicial, se realizaron reaislamientos. Resultados y discusión. En las pruebas de patogenicidad, 100% de los aislados formaron micelio aéreo, crecimientos en color blanco y polvo negro sobre los dátiles. Se observaron al microscopio colonias negras, con conidióforos marrones, vesículas globosas de 80 µm de diámetro aproximadamente, y conidios de alrededor de 5 µm (Barnett y Hunter, 1998). Basado en la caracterización morfológica, se encontró que los síntomas en frutos eran ocasionados por Aspergillus niger, similares a los observados en otras regiones productoras de dátil (Goudarzi et al., 2022). Futuras pruebas de identificación molecular complementarias son requeridas. Conclusión. Se identificó la presencia de A. niger como agente causal de moho negro en dátiles durante la poscosecha en Valle de Mexicali.

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Aspergillus niger, the causal agent of black mould disease in date fruits, infects and colonizes flowers and young fruitlets

Cited by 9 publications

References 23 publications

Bioactive Secondary Metabolites from Aspergillus fumigatus ON428521 Isolated from Wadi El Rayan, El Fayum Governorate

Bioactive Secondary Metabolites from Aspergillus fumigatus ON428521 Isolated from Wadi El Rayan, El Fayum Governorate

Exploration of Antagonistic Fungi From Rhizospheric Soil Against Phytopathogens of Solanum melongena and Citrus sinensis

Aislamiento e identificación de hongos fitopatógenos en dátiles en poscosecha en Valle de Mexicali, México

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