Aspergillus cristatus attenuates DSS‐induced intestinal barrier damage through reducing the oxidative stress, regulating short‐chain fatty acid and inhibiting MAPK signaling pathways

Abstract

BACKGROUND Probiotics are regarded as a promising strategy for relieving colitis caused by dextran sulfate sodium (DSS). One of the dominant probiotic fungi in Fuzhuan brick tea is identified as Aspergillus cristatus, but whether it can effectively improve colitis remains poorly understood. Here, the improving effect of A. cristatus on colitis was investigated. RESULTS Our results showed that A. cristatus intervention prominently alleviated gut damage as evidenced by the inhibition of shortened colon length, goblet cell depletion, and histological injury. Mechanistically, after administration with low concentrations of A. cristatus H‐1 and A. cristatus S‐6, the expression of interleukin‐6, tumor necrosis factor‐α, interleukin‐1β, nitric oxide, and malondialdehyde were significantly downregulated, and the content of glutathione, catalase, interleukin‐10, immunoglobulin G, claudin‐1, occludin, and zonula occludens‐1 were effectively upregulated. More importantly, live A. cristatus supplementation lightened DSS‐induced gut barrier damage by suppressing activation of the mitogen‐activated protein kinase (MAPK) signaling pathway, increasing the synthesis of short‐chain fatty acids (SCFAs) and stimulating the increase in peroxisome proliferator‐activated receptor γ expression. CONCLUSION Together, A. cristatus can attenuate DSS‐induced intestinal barrier damage through reducing the oxidative stress, regulating SCFA and inhibiting MAPK signaling pathways (P38/JNK/ERK). Our findings indicate that A. cristatus replenishment has potential as a new probiotic fungi to reduce DSS‐induced colitis. © 2022 Society of Chemical Industry.

Preventive effect of tilapia skin collagen hydrolysates on ulcerative colitis mice based on metabonomic and 16 S rRNA gene sequencing

“…Moreover, the standard error of the medium dose of TSCHs (500 mg kg −1 ) was relatively large, which may be caused by individual differences in animals. Such a result was similar to that reported by Wang et al 60 . and Hu et al 61 .…”

“…Moreover, the standard error of the medium dose of TSCHs (500 mg kg −1 ) was relatively large, which may be caused by individual differences in animals. Such a result was similar to that reported by Wang et al 60 and Hu et al 61 It is suggested that TSCHs may inhibit the activation of inflammatory signaling pathways associated with IL-6 to reduce the production of IL-6, sequentially attenuating intestinal mucosal damage and IL-6 cytokine release.…”

Alpha-lipoic acid, apocynin or probiotics influence glutathione status and selected inflammatory parameters in C57/BL6 mice when combined with a low-fat diet

“…Probiotics inhibit the inflammatory process by lowering the activity of nuclear factor kappa B (NF-κB), resulting in a decrease in tumor necrosis factor-alpha (TNF-α), interleukin 1, 6 (IL-1, IL-6), and C-reactive protein (CRP) production. It is worth noting that probiotics stimulate the activity of GSH and CAT [ 14 ], reduce the production of ROS in the intestines, and remove peroxides, heavy metals, and hydroxyl radicals [ 15 – 17 ]. The administration of probiotics protects against the development of oxidative stress [ 14 – 16 ] in the course of obesity-associated asthma.…”

“…It is worth noting that probiotics stimulate the activity of GSH and CAT [ 14 ], reduce the production of ROS in the intestines, and remove peroxides, heavy metals, and hydroxyl radicals [ 15 – 17 ]. The administration of probiotics protects against the development of oxidative stress [ 14 – 16 ] in the course of obesity-associated asthma.…”