<i>ARGONAUTE 2</i> increases rice susceptibility to rice black‐streaked dwarf virus infection by epigenetically regulating <i>HEXOKINASE 1</i> expression

Wang, Zhaoyun; Chen, Dongyue; Sun, Feng; Guo, Wei; Wang, Wei; Li, Xuejuan; Lan, Ying; Du, Linlin; Li, Shuo; Fan, Yanting; Zhou, Yijun; Zhao, Hongwei; Zhou, Tong

doi:10.1111/mpp.13091

Cited by 28 publications

(22 citation statements)

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“…Constant induction of AtHXK1 and 2 transcripts in Arabidopsis resulted in elevated resistance to the infections of necrotrophic fungi ( Alternaria brassicicola ) and bacterial P. syringae , indicating the defense and immunity roles of HXK against various pathogens [ 40 , 46 ]. The underlying mechanisms of HXK involved in regulating immunity and defense appeared to be related to altered ROS accumulation, further supported by several recent works performed on rice and apples [ 47 , 48 ].…”

Section: Discussionmentioning

confidence: 53%

“…tomato DC3000 through the glucose effect mediated by AtHXK1-related pathways [ 46 ]. Overexpression of native OsHXK1 in rice led to improved ROS accumulation and leaf resistance to virus infection [ 47 ]. Moreover, a recent report demonstrated that constant induction of MdHXK1 expression in apple ( Malus domestica ) enhanced resistance to ring rot fungi pathogen and ROS production related to glucose signaling [ 48 ].…”

Section: Introductionmentioning

confidence: 99%

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Genome-Wide Survey and Expression Analyses of Hexokinase Family in Poplar (Populus trichocarpa)

Han

Xiong

et al. 2022

Plants

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Hexokinase (HXK) family proteins exert critical roles in catalyzing hexose phosphorylation, sugar sensing, and modulation of plant growth and stress adaptation. Nevertheless, a large amount remains unknown about the molecular profile of HXK enzymes in Populus trichocarpa, a woody model tree species. A genome-wide survey of HXK-encoding genes, including phylogenies, genomic structures, exon/intron organization, chromosomal distribution, and conserved features, was conducted, identifying six putative HXK isogenes (PtHXK1-6) in the Populus genome. The evolutionary tree demonstrated that 135 homologous HXKs between 17 plant species were categorized into four major subfamilies (type A, B, C, and D), clustering one plastidic (PtHXK3) and five mitochondrial PtHXKs grouped into type A and B, respectively. The in silico deduction prompted the presence of the conserved sugar-binding core (motif 4), phosphorylation sites (motif 2 and 3), and adenosine-binding domains (motif 7). The transcriptomic sequencing (RNA-seq) and the quantitative real-time PCR (qRT-PCR) assays revealed that three isogenes (PtHXK2, 3, and 6) were abundantly expressed in leaves, stems, and roots, while others appeared to be dominantly expressed in the reproductive tissues. Under the stress exposure, PtHXK2 and 6 displayed a significant induction upon the pathogenic fungi (Fusarium solani) infection and marked promotions by glucose feeding in roots. In contrast, the PtHXK3 and 6 are ABA-responsive genes, following a dose-dependent manner. The comprehensive analyses of the genomic patterns and expression profiling provide theoretical clues and lay a foundation for unraveling the physiological and signaling roles underlying the fine-tuned PtHXKs responding to diverse stressors.

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Section: Discussionmentioning

confidence: 53%

Section: Introductionmentioning

confidence: 99%

Genome-Wide Survey and Expression Analyses of Hexokinase Family in Poplar (Populus trichocarpa)

Han

Xiong

et al. 2022

Plants

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“…Protein in each sample was separated in sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS‐PAGE) gels through electrophoresis and then transferred onto polyvinylidene fluoride (PVDF) membranes. Antibody against FLAG was purchased from Sigma‐Aldrich (Wang et al ., 2021).…”

Section: Methodsmentioning

confidence: 99%

An aspartic protease 47 causes quantitative recessive resistance to rice black‐streaked dwarf virus disease and southern rice black‐streaked dwarf virus disease

et al. 2022

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Summary Rice black‐streaked dwarf virus disease (RBSDVD) and southern rice black‐streaked dwarf virus disease (SRBSDVD) are the most destructive viral diseases in rice. Progress is limited in breeding due to lack of resistance resource and inadequate knowledge on the underlying functional gene. Using genome‐wide association study (GWAS), linkage disequilibrium (LD) decay analyses, RNA‐sequencing, and genome editing, we identified a highly RBSDVD‐resistant variety and its first functional gene. A highly RBSDVD‐resistant variety W44 was identified through extensive evaluation of a diverse international rice panel. Seventeen quantitative trait loci (QTLs) were identified among which qRBSDV6‐1 had the largest phenotypic effect. It was finely mapped to a 0.8–1.2 Mb region on chromosome 6, with 62 annotated genes. Analysis of the candidate genes underlying qRBSDV6‐1 showed high expression of aspartic proteinase 47 (OsAP47) in a susceptible variety, W122, and a low resistance variety, W44. OsAP47 overexpressing lines exhibited significantly reduced resistance, while the knockout mutants exhibited significantly reduced SRBSDVD and RBSDVD severity. Furthermore, the resistant allele Hap1 of OsAP47 is almost exclusive to Indica, but rare in Japonica. Results suggest that OsAP47 knockout by editing is effective for improving RBSDVD and SRBSDVD resistance. This study provides genetic information for breeding resistant cultivars.

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“…A rice LRR receptor-like protein and its adaptor kinase SOBIR1 were reported to be involved in immunity against RBSDV infection [ 13 ]. Rice ARGONAUTE (AGO) proteins play crucial roles in plant defense against RBSDV invasion by regulating HEXOKINASE 1 expression [ 14 ].…”

Section: Introductionmentioning

confidence: 99%

Functional analysis revealed the involvement of ZmABCB15 in resistance to rice black-streaked dwarf virus infection

et al. 2022

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Background Maize rough dwarf disease (MRDD), caused by rice black-streaked dwarf virus (RBSDV) belonging to the Fijivirus genus, seriously threatens maize production worldwide. Three susceptible varieties (Ye478, Zheng 58, and Zhengdan 958) and two resistant varieties (P138 and Chang7–2) were used in our study. Results A set of ATP-binding cassette subfamily B (ABCB) transporter genes were screened to evaluate their possible involvements in RBSDV resistance. In the present study, ZmABCB15, an ABCB transporter family member, was cloned and functionally identified. Expression analysis showed that ZmABCB15 was significantly induced in the resistant varieties, not in the susceptible varieties, suggesting its involvement in resistance to the RBSDV infection. ZmABCB15 gene encodes a putative polar auxin transporter containing two trans-membrane domains and two P-loop nucleotide-binding domains. Transient expression analysis indicated that ZmABCB15 is a cell membrance localized protein. Over-expression of ZmABCB15 enhanced the resistance by repressing the RBSDV replication ratio. ZmABCB15 might participate in the RBSDV resistance by affecting the homeostasis of active and inactive auxins in RBSDV infected seedlings. Conclusions Polar auxin transport might participate in the RBSDV resistance by affecting the distribution of endogenous auxin among tissues. Our data showed the involvement of polar auxin transport in RBSDV resistance and provided novel mechanism underlying the auxin-mediated disease control technology.

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ARGONAUTE 2 increases rice susceptibility to rice black‐streaked dwarf virus infection by epigenetically regulating HEXOKINASE 1 expression

Abstract: This is an open access article under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

Cited by 28 publications

References 54 publications

Genome-Wide Survey and Expression Analyses of Hexokinase Family in Poplar (Populus trichocarpa)

Genome-Wide Survey and Expression Analyses of Hexokinase Family in Poplar (Populus trichocarpa)

An aspartic protease 47 causes quantitative recessive resistance to rice black‐streaked dwarf virus disease and southern rice black‐streaked dwarf virus disease

Functional analysis revealed the involvement of ZmABCB15 in resistance to rice black-streaked dwarf virus infection

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