<i>ARG1</i>Gene Polymorphisms and Their Association in Individuals with Essential Hypertension: A Case–Control Study

Shah, Syed Fawad Ali; Iqbal, Tahir; Qamar, Raheel; Rafiq, Muhammad; Hussain, Shahzad

doi:10.1089/dna.2018.4222

Cited by 19 publications

(13 citation statements)

References 27 publications

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“…Approximately 95% of the cases are classed as essential hypertension (EH) (2)(3)(4); however, to date, the exact etiology and pathogenesis of EH have not been fully elucidated. Recent studies have found that the pathogenic factors of EH do not only involve the interaction between genetic and environmental factors (5,6), but are also correlated with human cytomegalovirus (HcMV) infection, microRNA (miRNA) regulation and inflammation activation, as well as other mechanisms (7)(8)(9).…”

Section: Introductionmentioning

confidence: 99%

MicroRNA‑1929‑3p participates in murine cytomegalovirus‑induced hypertensive vascular remodeling through Ednra/NLRP3 inflammasome activation

Zhou

Shi

et al. 2020

Int J Mol Med

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MicroRNAs (miRNAs) play an important role in the development of vascular remodeling in essential hypertension (EH) by mediating the effects of human cytomegalovirus (HCMV) on the vascular system. Therefore, the aim of the present study was to investigate the effects of murine cytomegalovirus (MCMV) infection on blood pressure and vascular function in mice, in order to elucidate the role of miR-1929-3p in this process. For model development, 7-month-old C57BL/6J mice were infected with the Smith strain of MCMV, and MCMV DNA, IgG and IgM were detected. Subsequently, blood pressure was measured via the carotid artery, and the morphological changes of the aorta were evaluated by hematoxylin and eosin and Masson's trichrome staining. miR-1929-3p transfection was performed using an adeno-associated virus packaged vector and the changes in vascular structure were then observed. The levels of nitric oxide (NO) and endothelial NO synthase were also assessed with colorimetry. Vascular remodeling and expression of NLRP3 inflammasome pathway-related proteins were detected by immunohistochemistry and western blotting. Endothelin-1 (ET-1), interleukin (IL)-1β and IL-18 were assayed by ELISA. The results revealed that MCMV infection increased the blood pressure, promoted vascular remodeling, caused endothelial cell injury, and downregulated miR-1929-3p. However, these effects were alleviated by miR-1929-3p overexpression, which downregulated endothelin A receptor (Ednra) and NLRP3 inflammasome, as well as endothelial injury- and vascular remodeling-related genes. Taken together, the findings of the present study indicated that overexpression of miR-1929-3p may improve MCMV-induced vascular remodeling, possibly via the deactivation of the NLRP3 inflammasome by ET-1/Ednra.

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Section: Introductionmentioning

confidence: 99%

MicroRNA‑1929‑3p participates in murine cytomegalovirus‑induced hypertensive vascular remodeling through Ednra/NLRP3 inflammasome activation

Zhou

Shi

et al. 2020

Int J Mol Med

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“…We found, for example, ARG1, ARG2 and HGF. In a study by Shah S. F. A. et al, the authors suggested that a polymorphism in ARG1 may be linked to essential hypertension 44 . ARG1 is generally expressed in macrophages, coronary endothelial cells and vascular smooth muscle aortic cells, while ARG2 is expressed in human aortic endothelial cells and human umbilical vein endothelial cells 45 .…”

Section: Resultsmentioning

confidence: 99%

Circulating RNA Transcriptome of Pregnant Women with TSH Just Above the Trimester-Specific Reference and its Correlation with the Hypertensive Phenotype

Hirata

Rocha

Silva

et al. 2020

Sci Rep

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During gestation, a woman's body undergoes physiological changes that alter thyroid function. pregnant women with hypothyroidism may exhibit gestational complications, including hypertension and preeclampsia. We investigated differentially expressed genes (DEGs) in circulating RNAs from pregnant women with tSH levels just above the normal range to determine the impact of a mild elevation of TSH in pregnancy. We selected three women with healthy thyroid pregnancy (HTP), three pregnant women with gestational hypothyroidism (GHT), and three nonpregnant women (NPG) to construct transcriptome libraries. We also compared our results with data from the GEO dataset and DisGeNET. We identified 1500 DEG in GHT and 1656 DEG in HTP. From GEO dataset, we recognized 453 DEGs in trimester-specific plasma RNA, 1263 DEGs in placental tissues from healthy women, 1031 DEGs from preeclamptic uteroplacental tissues and 1657 DEGs from placental tissues from severely preeclamptic women. In this scenario, 12.26% and 12.86% genes were shared between these datasets in GHT and HTP, respectively. We stablished 62 genes in GHT DEGs related to hypertensive phenotype hallmarks. In conclusion, even in women with a mild TSH increment, we were able to detect some DEGs that could be associated with a hypertensive phenotype.Circulating Ribonucleic Acid (RNA) represents a powerful strategy, less invasive and capable of real-time tracking diseases. The circulating protein codifying transcripts (mRNA, messenger RNA) may also provide clinically useful additional information when compared to the direct protein measurement 1 . This strategy is also sensible enough to detect fetal RNA circulating in the mother's blood 2 . The use of corticotropin-releasing hormone (CRH) mRNA detection in pregnancy was also reported to be capable of working as a molecular marker for preeclampsia 3 . Nevertheless, the transcriptome analysis is efficient enough to offer much more information than the use of a single RNA. The target RNA detection strategies, like RNA-Seq, can be performed in a simple blood sample and can give us the quantitative expression of the coding and noncoding RNA from the mother, the placenta or the fetus in a unique and noninvasive way 4 .During gestation, a woman's body undergoes physiological changes that alter thyroid function and thyroid hormone metabolism. Most of these changes are related to the increased demand for thyroid hormone by the mother and the fetus, the placental degradation of the hormone and the increase in the thyroxin-binding globulin (TBG) concentration. TBG increases due to an estrogenic stimulus during pregnancy, and for this reason, free thyroxine (FT 4 ) is highly influenced by the significant increase in the thyroxine (T 4 )-bound fraction.

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“…Altered expression of SLC6A9 [56] and RHD (Rh blood group D antigen) [57], CHRFAM7A [58], ANXA3 [59], SLC1A5 [60], KCNH2 [61], HP (haptoglobin) [62], SELENBP1 [63], SNCA (synuclein alpha) [64], TGM2 [65], PINK1 [66], B2M [67], QPCT (glutaminyl-peptide cyclotransferase) [68], CBS (cystathionine beta-synthase) [69], NQO2 [70], GLRX5 [71], BASP1 [72], GAS7 [73], GPX1 [74], OLIG2 [75], RPTN (repetin) [76], IL33 [77], SOX10 [78], GRIK1 [79], ZFPM2 [80], SHANK3 [81], ERBB3 [82], ARC (activity regulated cytoskeleton associated protein) [83], GFAP (glial fibrillary acidic protein) [84], PLAT (plasminogen activator, tissue type) [85], GRIK5 [86], CACNB2 [87], NRXN2 [88], TERT (telomerase reverse transcriptase) [89], GNB3 [90], L1CAM [91], EGR3 [92], CAV1 [93], CACNA1B [94], MAGI1 [95], KIR2DL1 [96], PAH (phenylalanine hydroxylase) [97] and CYP3A5 [98] have been shown in schizophrenia. Recent studies showed that SLC6A9 [99], FKBP1B [100], S100A12 [101], SLC6A19 [102], TXN (thioredoxin) [103], TLR9 [104], HP (haptoglobin) [105], ARG1 [106], PINK1 [107], B2M [108], C5AR1 [109], MYADM (myeloid associated differentiation marker) [110], CBS (cystathionine beta-synthase) [111], GPX1 [112], SIAH2 [113], PRDX2 [114], RDH8 [115], CYP11B2 [116], RARRES2 [117], NOX1 [118], IL33 [119], OTC (ornithine transcarbamylase) [120], CYP1A1 [121], NFATC4 [122], TSLP (thymic stromal lymphopoietin) [123], WNT4 [124], MGP (matrix Gla protein) [125], FGFBP1 […”

Section: Discussionmentioning

confidence: 99%

Identification of Key Genes and Biological Pathways in Bipolar Disorder by Bioinformatics and Next Generation Sequencing Data Analysis

Vastrad¹,

Vastrad²

2022

Preprint

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Bipolar disorder (BD), also known as psychiatric disorder, affects millions of people all over the world. The aim of this investigation was to screen and verify hub genes involved in BD as well as to explore potential molecular mechanisms. The next generation sequencing (NGS) dataset GSE124326 was downloaded from the Gene Expression Omnibus (GEO) database, which contained 480 samples, including 240 BD and 240 normal controls. Differentially expressed genes (DEGs) were filtered and subjected to gene ontology (GO) and pathway enrichment analyses. A protein–protein interaction (PPI) network and module analysis were used to identify hub genes. The miRNA-hub gene regulatory network and TF-hub gene regulatory network were also constructed. Receiver operating characteristic curve (ROC) analysis was used to validate hub genes. In this work, 957 DEGs, including 477 up regulated and 480 down regulated, were obtained from NGS data. The GO and pathway enrichment analyses of the DEGs showed that the up regulated genes were enriched in the neutrophil degranulation, immune system, transport, cytoplasm and enzyme regulator activity, and the down regulated genes were enriched in extracellular matrix organization, diseases of metabolism, multicellular organismal process, cell periphery and metal ion binding. Finally, through analyzing the PPI network, miRNA-hub gene regulatory network and TF-hub gene regulatory network, we screened hub genes UBB, UBE2D1, TUBA1A, RPL11, RPS24, NOTCH3, CAV1, CNBD2, CCNA1 and MYH11 by the Cytoscape software. The current investigation illustrates a characteristic NGS data in BD, which might contribute to the interpretation of the progression of BD and provide novel biomarkers and therapeutic targets for BD.

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ARG1Gene Polymorphisms and Their Association in Individuals with Essential Hypertension: A Case–Control Study

Cited by 19 publications

References 27 publications

MicroRNA‑1929‑3p participates in murine cytomegalovirus‑induced hypertensive vascular remodeling through Ednra/NLRP3 inflammasome activation

MicroRNA‑1929‑3p participates in murine cytomegalovirus‑induced hypertensive vascular remodeling through Ednra/NLRP3 inflammasome activation

Circulating RNA Transcriptome of Pregnant Women with TSH Just Above the Trimester-Specific Reference and its Correlation with the Hypertensive Phenotype

Identification of Key Genes and Biological Pathways in Bipolar Disorder by Bioinformatics and Next Generation Sequencing Data Analysis

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