<i>Arabidopsis thaliana</i> FANCD2 Promotes Meiotic Crossover Formation

Kurzbauer, Marie-Therese; Pradillo, Mónica; Kerzendorfer, Claudia; Sims, Jason; Ladurner, René; Oliver, Cecilia; Janisiw, Michael; Mosiołek, Magdalena; Schweizer, Dieter; Copenhaver, Gregory P.; Schlögelhofer, Peter

doi:10.1105/tpc.17.00745

Cited by 49 publications

(43 citation statements)

References 102 publications

(119 reference statements)

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“…COs occurring via this pathway are specifically marked by Zip3/Hei10 and MLH1 foci at late pachytene (Agarwal and Roeder 2000;Lhuissier et al 2007;Chelysheva et al 2012). A number of secondary "clean-up" pathways repair DSBs not metabolized by the class I pathway (Hollingsworth and Brill 2004;Kurzbauer et al 2018). These clean-up pathways mostly repair DSBs as non-COs, but also contribute a smaller number of COs (i.e., class II COs).…”

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confidence: 99%

Modelling Sex-Specific Crossover Patterning inArabidopsis

Lloyd

Jenczewski

2019

Genetics

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“Interference” is a major force governing the patterning of meiotic crossovers. A leading model describing how interference influences crossover patterning is the beam-film model, a mechanical model based on the accumulation and redistribution of crossover-promoting “stress” along the chromosome axis. We use the beam-film model in conjunction with a large Arabidopsis reciprocal backcross data set to gain “mechanistic” insights into the differences between male and female meiosis, and crossover patterning. Beam-film modeling suggests that the underlying mechanics of crossover patterning and interference are identical in the two sexes, with the large difference in recombination rates and distributions able to be entirely explained by the shorter chromosome axes in females. The modeling supports previous indications that fewer crossovers occur via the class II pathway in female meiosis and that this could be explained by reduced DNA double-strand breaks in female meiosis, paralleling the observed reduction in synaptonemal complex length between the two sexes. We also demonstrate that changes in the strength of suppression of neighboring class I crossovers can have opposite effects on “effective” interference depending on the distance between two genetic intervals.

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confidence: 99%

Modelling Sex-Specific Crossover Patterning inArabidopsis

Lloyd

Jenczewski

2019

Genetics

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“…In the wild type, of the ~200 DSBs initiated in early prophase (Chelysheva et al, 2012;Choi et al, 2013;Girard et al, 2015;Seguela-Arnaud et al, 2015) around half are thought to be processed by the ZMM proteins (Higgins et al, 2004;Higgins et al, 2008a;Chelysheva et al, 2012) with the remaining breaks processed by parallel non-ZMM pathways e.g. via MUS81 or FANCD2 (Kurzbauer et al, 2018) (Figure 9). A small percentage of the joint molecules processed by these secondary pathways are eventually resolved as class II COs .…”

Section: Cdkg1 Promotes the Stability Of Early Recombination Intermedmentioning

confidence: 99%

“…Another possible explanation is that in the absence of CDKG1, joint molecules that enter the class II pathways have a greater likelihood of being repaired as COs. In either situation, the recombination intermediates are processed by a MUS81-independent pathway, likely involving FANCD2 (Kurzbauer et al, 2018), as the double cdkg1-1mus81-2 mutant behaves as the single cdkg1-1 mutant.…”

Section: Cdkg1 Promotes the Stability Of Early Recombination Intermedmentioning

confidence: 99%

“…A number of additional pathways repair DSBs that are not metabolized by the ZMM proteins. In most cases, these DSB repair pathways resolve joint molecules as NCOs, but they also contribute a small number of COs, mostly mediated by either MUS81 (MMS and UV sensitive 81) (Berchowitz et al, 2007;Higgins et al, 2008a) or FANCD2 (Fanconi anemia D2) (Kurzbauer et al, 2018). These class II COs are insensitive to interference and usually make up 10-20% of the total CO number (Housworth and Stahl, 2003;Basu-Roy et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

“…This implies that there are mechanisms designating which DSBs become resolved as COs and that there are inhibitory mechanisms preventing resolution of DSBs into COs at the remaining sites. While the factors involved in CO designation remain elusive, recent studies have identified anti-CO factors that include the DNA helicases RECQ4A and RECQ4B, Topoisomerase3α, RMI (RECQ Mediated Genome Instability 1), the DNA helicase FANCM and its co-factors MHF1 and MFH2 (FANCM interacting histone-fold protein), the AAA-ATPase Fidgetin-like1 (FIGL1) and its interacting protein FLIP (FIDGETIN-LIKE-1 INTERACTING PROTEIN) (Crismani et al, 2012;Dangel et al, 2014;Girard et al, 2014;Girard et al, 2015;Seguela-Arnaud et al, 2015;Seguela-Arnaud et al, 2017;Fernandes et al, 2018;Kurzbauer et al, 2018;Serra et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

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CDKG1 Is Required for Meiotic and Somatic Recombination Intermediate Processing in Arabidopsis

et al. 2020

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Short title: The role of CDKG1 in recombinationOne-sentence summary: The cyclin-dependent kinase CDKG1 stabilises recombination intermediates during male meiosis and DNA damage-induced somatic homologous recombination. ABSTRACTThe Arabidopsis thaliana cyclin-dependent kinase G1 (CDKG1) is necessary for recombination and synapsis during male meiosis at high ambient temperature. In the cdkg1-1 mutant, synapsis is impaired and there is a dramatic reduction in the number of class I crossovers resulting in univalents at metaphase I and pollen sterility. Here we demonstrate that CDKG1 is necessary for the processing of recombination intermediates in the canonical ZMM recombination pathway and that loss of CDKG1 results in increased class II crossovers. While synapsis and events associated with class I crossovers are severely compromised in a cdkg1-1 mutant, they can be restored by increasing the number of recombination intermediates in the double cdkg1-1 fancm-1 mutant. Despite this, recombination intermediates are not correctly resolved, leading to the formation of chromosome aggregates at metaphase I. Our results show that CDKG1 acts early in the recombination process and is necessary to stabilize recombination intermediates. Finally, we show that the effect on recombination is not restricted to meiosis and that CDKG1 is also required for normal levels of DNA damage-induced homologous recombination in somatic tissues. . Both ATM and ATR promote the efficient and accurate processing of programmed meiotic double-strand breaks. The Plant Journal 55, 629-638. Dangel, N.J., Knoll, A., and Puchta, H. (2014). MHF1 plays Fanconi anaemia complementation group M protein (FANCM)-dependent and FANCM-independent roles in DNA repair and homologous recombination in plants.

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Plant reproduction research in Latin America: Toward sustainable agriculture in a changing environment

Ronceret,

Bolaños‐Villegas

2024

Plant Enviro Interactions

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Food production and food security depend on the ability of crops to cope with anthropogenic climate change and successfully produce seed. To guarantee food production well into the future, contemporary plant scientists in Latin America must carry out research on how plants respond to environmental stressors such as temperature, drought, and salinity. This review shows the opportunities to apply these results locally and abroad and points to the gaps that still exist in terms of reproductive processes with the purpose to better link research with translational work in plant breeding and biotechnology. Suggestions are put forth to address these gaps creatively in the face of chronic low investment in science with a focus on applicability.

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Arabidopsis thaliana FANCD2 Promotes Meiotic Crossover Formation

Abstract: promotes non-interfering COs in a MUS81-independent manner and is therefore part of an 47 uncharted meiotic CO-promoting mechanism, in addition to those described previously. 48

Cited by 49 publications

References 102 publications

Modelling Sex-Specific Crossover Patterning inArabidopsis

Modelling Sex-Specific Crossover Patterning inArabidopsis

CDKG1 Is Required for Meiotic and Somatic Recombination Intermediate Processing in Arabidopsis

Plant reproduction research in Latin America: Toward sustainable agriculture in a changing environment

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