<i>Arabidopsis thaliana</i>
            as Model for Studies on the Bacterial Root Microbiota

Schlaeppi, Klaus; Themaat, Emiel Ver Loren van; Bulgarelli, Davide; Schulze‐Lefert, Paul

doi:10.1002/9781118297674.ch23

Cited by 7 publications

(3 citation statements)

References 77 publications

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“…Root exudates can also influence rhizosphere interactions serving as signals that initiate symbiotic relationships that help plants to tolerate abiotic stresses, stimulate growth, or induce plant defenses against pathogens and insects ( Mendes et al, 2011 ; Pieterse et al, 2014 ; Pérez-Jaramillo et al, 2016 ) or mediate negative interactions including association with parasitic plants, pathogenic microbes, and invertebrate herbivores ( Badri and Vivanco, 2009 ). The variation in the rhizospheric microbial community in different types of soils is attributed to the soil background ( Schlaeppi et al, 2013 ) thus the soil is a determining factor in the formation of rhizosphere microbiome ( Garbeva et al, 2008 ; Lundberg et al, 2012 ). As in our study the compost was proven to be suppressive against the vascular wilt diseases of tomato, our secondary goal was to understand whether this suppressiveness was due to differences in the microbial composition of the rhizosphere and the unplanted compost.…”

Section: Discussionmentioning

confidence: 99%

Rhizosphere Microbiome Recruited from a Suppressive Compost Improves Plant Fitness and Increases Protection against Vascular Wilt Pathogens of Tomato

et al. 2017

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Suppressive composts represent a sustainable approach to combat soilborne plant pathogens and an alternative to the ineffective chemical fungicides used against those. Nevertheless, suppressiveness to plant pathogens and reliability of composts are often inconsistent with unpredictable effects. While suppressiveness is usually attributed to the compost’s microorganisms, the mechanisms governing microbial recruitment by the roots and the composition of selected microbial communities are not fully elucidated. Herein, the purpose of the study was to evaluate the impact of a compost on tomato plant growth and its suppressiveness against Fusarium oxysporum f. sp. lycopersici (Foxl) and Verticillium dahliae (Vd). First, growth parameters of tomato plants grown in sterile peat-based substrates including 20 and 30% sterile compost (80P/20C-ST and 70P/30C-ST) or non-sterile compost (80P/20C and 70P/30C) were evaluated in a growth room experiment. Plant height, total leaf surface, and fresh and dry weight of plants grown in the non-sterile compost mixes were increased compared to the plants grown in the sterile compost substrates, indicating the plant growth promoting activity of the compost’s microorganisms. Subsequently, compost’s suppressiveness against Foxl and Vd was evaluated with pathogenicity experiments on tomato plants grown in 70P/30C-ST and 70P/30C substrates. Disease intensity was significantly less in plants grown in the non-sterile compost than in those grown in the sterile compost substrate; AUDPC was 2.3- and 1.4-fold less for Foxl and Vd, respectively. Moreover, fungal quantification in planta demonstrated reduced colonization in plants grown in the non-sterile mixture. To further investigate these findings, we characterized the culturable microbiome attracted by the roots compared to the unplanted compost. Bacteria and fungi isolated from unplanted compost and the rhizosphere of plants were sequence-identified. Community-level analysis revealed differential microbial communities between the compost and the rhizosphere, suggesting a clear effect of the plant in the microbiome assembly. Proteobacteria and Actinobacteria were highly enriched in the rhizosphere whereas Firmicutes were strongly represented in both compartments with Bacillus being the most abundant species. Our results shed light on the composition of a microbial consortium that could protect plants against the wilt pathogens of tomato and improve plant overall health.

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Section: Discussionmentioning

confidence: 99%

Rhizosphere Microbiome Recruited from a Suppressive Compost Improves Plant Fitness and Increases Protection against Vascular Wilt Pathogens of Tomato

et al. 2017

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“…Vuong et al [72] demonstrated that the host species significantly influence rhizobia community structure in acacia nodules. Additionally, the identity of rhizobia species capable of forming a symbiotic relationship with the host plant is influenced by both the composition and structure of the indigenous microbial community [73] and the nature, structure, physical and chemical characteristics of the soil [74].…”

Section: Discussionmentioning

confidence: 99%

Phylogenetic diversity of the rhizobial nodulating symbionts associated to the introduced Vachellia karroo (Hayne) Banfi and Galasso (Fabaceae) in Algeria

Amina,

Samir,

Thinhinan

et al. 2024

Preprint

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The diversity of rhizobia associated with the introduced woody legume flora of Algeria were rarely investigated despite their widespread distribution and agroecological and economic importance. The current research attempted to fill this knowledge gap by identifying the rhizobia species associated with Vachellia karoo seedlings cultivated in ten nurseries distributed in different bioclimatic stages all over the country. The primary objective was to understand the diversity and distribution of rhizobia species associated with this legume across different soil and climate zones. The results of our study show that despite the fact that Vachellia karoo species are located exclusively in the north part of the country under a humid and sub humid climate, the seedlings cultivated in ten nurseries distributed in different bioclimatic stages (Saharian, sub Saharian and humid) have a strong ability to grow and form nitrogen-fixing nodules regardless of the soil and climate zone. The molecular identification based on the 16S RNA gene of the 29 strains isolated and identified in the laboratory were assigned to three genera: Ensifer, Mesorhizobium and Bradyrhizobium. The genus Ensifer showed a higher occurrence (48%) and diversity compared to the two other genera and an exclusive symbiotic association with Vachellia karroo seedlings collected from nurseries located in saharian and sub saharian zones.

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“…Zarraonaindia studies showed that as a potential microbial pool of plant-related microorganisms, soil microorganisms have a great impact on grape root related microorganisms [68]. Soil type is an important factor affecting the rhizosphere microbial community, and the main reason for the in uence is the difference of soil microbial community [69]. Therefore, in our previous analysis, we also found that the most important environmental factor affecting the bacterial community of Dendrobium is also soil factor, accounting for 25.31%.…”

Section: Soil Is the Most Important Environmental Factor Affecting Thmentioning

confidence: 99%

Bacterial Composition and Diversity Associated with Rhizosphere of the Chinese Medicinal Herb Dendrobium

Yuan

Liu

et al. 2020

Preprint

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Background: Dendrobium is a precious herbal belongs to Orchid and widely used as health care traditional Chinese medicine in Asia. Although orchids are mycorrhizal plants, most researches still focus on endophytes, and there is still large unknown in rhizosphere microorganisms. In order to investigate the rhizosphere microbial community of different Dendrobium species during the maturity stage, we used high-throughput sequencing to analyze microbial community in rhizosphere soil during maturity stage of three kinds of Dendrobium species.Results: In our study, a total of 240,320 sequences and 11,179 OTUs were obtained from these three Dendrobium species. According to the analysis of OTU annotation results, different Dendrobium rhizosphere soil bacteria include 2 kingdoms, 63 phyla, 72 classes, 159 orders, 309 families, 850 genera and 663 species. Among all sequences, the dominant bacterial phyla (relative abundance > 1%) were Proteobacteria, Actinobacteria, Bacteroidetes, Acidobacteria, Firmicutes, Verrucomicrobia, Planctomycetes, Chloroflexi, Gemmatimonadetes. We analyzed the environmental factors of the growth of Dendrobium and found that the environmental factor that affects the rhizosphere soil microorganisms of Dendrobium is the soil factor. Among them, soil factors most closely related to the influence of Dendrobium rhizosphere soil microorganisms include total nitrogen, available phosphorus, ammonium nitrogen and pH value.Conclusions: We found that the rhizosphere bacterial communities of the three kinds of Dendrobium have significant differences, and the main species of rhizosphere microorganisms of Dendrobium are concentrated in the Proteobacteria, Actinobacteria, Bacteroidetes. Moreover, the smaller the level of bacterial, the greater the difference among Dendrobium species. Soil is the most important environmental factor affecting the bacterial communities in the rhizosphere soil of Dendrobium. These results fill the gap in the rhizosphere microbial community of Dendrobium and provide a theoretical basis for the subsequent mining of microbial functions and the study of biological fertilizers.

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Arabidopsis thaliana as Model for Studies on the Bacterial Root Microbiota

Cited by 7 publications

References 77 publications

Rhizosphere Microbiome Recruited from a Suppressive Compost Improves Plant Fitness and Increases Protection against Vascular Wilt Pathogens of Tomato

Rhizosphere Microbiome Recruited from a Suppressive Compost Improves Plant Fitness and Increases Protection against Vascular Wilt Pathogens of Tomato

Phylogenetic diversity of the rhizobial nodulating symbionts associated to the introduced Vachellia karroo (Hayne) Banfi and Galasso (Fabaceae) in Algeria

Bacterial Composition and Diversity Associated with Rhizosphere of the Chinese Medicinal Herb Dendrobium

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