Arabidopsis ribosomal proteins control developmental programs through translational regulation of auxin response factors

Abstract

Upstream ORFs are elements found in the 5′-leader sequences of specific mRNAs that modulate the translation of downstream ORFs encoding major gene products. In Arabidopsis, the translational control of auxin response factors (ARFs) by upstream ORFs has been proposed as a regulatory mechanism required to respond properly to complex auxin-signaling inputs. In this study, we identify and characterize the aberrant auxin responses in specific ribosomal protein mutants in which multiple ARF transcription factors are simultaneously repressed at the translational level. This characteristic lends itself to the use of these mutants as genetic tools to bypass the genetic redundancy among members of the ARF family in Arabidopsis. Using this approach, we were able to assign unique functions for ARF2, ARF3, and ARF6 in plant development.ribosomal mutants | uORFs | auxin regulation

Arabidopsis ribosomal proteins control vacuole trafficking and developmental programs through the regulation of lipid metabolism

“…However, the result from the same group showed that mutation in the ARF2 gene disturbs vacuolar trafficking of PIN2 protein (23). Interestingly, our previous report elucidated that RPL4 regulates translational efficiency of ARF2, ARF3, ARF7, and ARF19 in a uORF-dependent mechanism (48). Based on these facts, it would be plausible to speculate that auxin signaling-regulated lipid metabolism requires active ARF transcriptional factors, which are under translational regulation by ribosome proteins.…”

The Arabidopsis Synaptotagmin1 Is Enriched in Endoplasmic Reticulum-Plasma Membrane Contact Sites and Confers Cellular Resistance to Mechanical Stresses

“…For microscopy assays, surface-sterilized and cold-stratified Arabidopsis (Arabidopsis thaliana) seeds were grown as described in Rosado et al (2012). For drug treatments, 4-d-old seedlings grown on solid one-half-strength Murashige and Skoog salts supplemented with 1% (w/v) Suc (basal medium) were transferred to 12-well plates containing liquid basal medium supplemented with 1 mM latrunculin B, 25 mM oryzalin, or mock (0.1% [v/v] dimethyl sulfoxide) and incubated for 24 h. After treatments, seedlings were rinsed and mounted on microscopy slides using water.…”

New Evidence for Differential Roles of L10 Ribosomal Proteins from Arabidopsis

“…Arabidopsis mutants in ribosomal proteins exhibit a large range of developmental phenotypes with extreme abnormalities, including embryonic lethality, suggesting that ribosomes also have specific functions regulating the expression of developmental genes (Van Lijsebettens et al, 1994;Degenhardt and Bonham-Smith, 2008;Byrne, 2009;Horiguchi et al, 2011Horiguchi et al, , 2012Szakonyi and Byrne, 2011). Furthermore, it has been recently demonstrated that ribosomal proteins control auxinmediated developmental programs by translational regulation of auxin response factors (Rosado et al, 2012). In addition, the characterization of single, double, and, in certain cases, triple mutants as well as complementation by paralog genes have demonstrated full, partial, and no redundancy between members of ribosomal protein families (Briggs et al, 2006;Guo and Chen, 2008;Guo et al, 2011;Horiguchi et al, 2011;Stirnberg et al, 2012).…”