<i>Arabidopsis</i>NAC1 transduces auxin signal downstream of TIR1 to promote lateral root development

Xie, Qi; Frugis, Giovanna; Colgan, Diana F.; Chua, Nam‐Hai

doi:10.1101/gad.852200

Cited by 834 publications

(823 citation statements)

References 39 publications

(31 reference statements)

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“…NAP (Sablowski and Meyerowitz 1998) from Arabidopsis acts as a target gene of AP3/PI to function in the transition between growth by cell division and cell expansion in stamens and petals. AtNAC1 mediates auxin signaling to promote lateral root development (Xie et al 2000). More recently, NAC proteins were found to be involved in plant responses to pathogen and environmental stimuli.…”

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confidence: 99%

Characterization of transcription factor gene SNAC2 conferring cold and salt tolerance in rice

et al. 2008

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Plants respond to adverse environment by initiating a series of signaling processes including activation of transcription factors that can regulate expression of arrays of genes for stress response and adaptation. NAC (NAM, ATAF, and CUC) is a plant specific transcription factor family with diverse roles in development and stress regulation. In this report, a stress-responsive NAC gene (SNAC2) isolated from upland rice IRA109 (Oryza sativa L. ssp japonica) was characterized for its role in stress tolerance. SNAC2 was proven to have transactivation and DNA-binding activities in yeast and the SNAC2-GFP fusion protein was localized in the rice nuclei. Northern blot and SNAC2 promoter activity analyses suggest that SNAC2 gene was induced by drought, salinity, cold, wounding, and abscisic acid (ABA) treatment. The SNAC2 gene was over-expressed in japonica rice Zhonghua 11 to test the effect on improving stress tolerance. More than 50% of the transgenic plants remained vigorous when all WT plants died after severe cold stress (4-8°C for 5 days). The transgenic plants had higher cell membrane stability than wild type during the cold stress. The transgenic rice had significantly higher germination and growth rate than WT under high salinity conditions. Over-expression of SNAC2 can also improve the tolerance to PEG treatment. In addition, the SNAC2-overexpressing plants showed significantly increased sensitivity to ABA. DNA chip profiling analysis of transgenic plants revealed many up-regulated genes related to stress response and adaptation such as peroxidase, ornithine aminotransferase, heavy metal-associated protein, sodium/hydrogen exchanger, heat shock protein, GDSL-like lipase, and phenylalanine ammonia lyase. Interestingly, none of the up-regulated genes in the SNAC2-overexpressing plants matched the genes up-regulated in the transgenic plants over-expressing other stress responsive NAC genes reported previously. These data suggest SNAC2 is a novel stress responsive NAC transcription factor that possesses potential utility in improving stress tolerance of rice.

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confidence: 99%

Characterization of transcription factor gene SNAC2 conferring cold and salt tolerance in rice

et al. 2008

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“…Furthermore, RT-qPCR results showed that the expression of miR164 target NAC1 gene was inversely proportional to miR164 expression (Figures 3 and 4). NAC1 is a transcription factor and specifically expressed in Arabidopsis roots (Xie et al 2000). Since V. dahliae is a soil-borne fungus and obligate in root infection, involvement of miR164 and NAC1 in regulation of V. dahliae infection is worthy of further investigation.…”

Section: Discussionmentioning

confidence: 99%

Genome-wide profiling of sRNAs in the Verticillium dahliae-infected Arabidopsis roots

et al. 2018

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Small RNAs (sRNAs, including small interfering RNAs [siRNAs] and micro RNAs [miRNAs]) are key mediators of RNA silencing (or RNA interference), which play important roles in plant development and response to biotic and abiotic stimulation. Verticillium wilt is a plant vascular disease caused by the soil-borne fungal pathogens, such as Verticillium dahliae. We previously reported that V. dahliae infection increased two plant endogenous miRNAs that were exported to fungal cell to silence virulence genes. To investigate plant sRNAs in genome-wide response to V. dahliae infection, in this study, we constructed two sRNA libraries from Arabidopsis roots with and without V. dahliae infection, respectively. In total, 31 conserved miRNAs were found to be differentially expressed during the early stage of infection with V. dahliae using sRNA sequencing. Among these, the expression levels of miR160, miR164, miR166, miR167, miR390 and miR156h were confirmed by northern blot. Reverse transcription quantitative real time polymerase chain reaction results showed that the induction of miRNAs (miR160, miR164, miR166 and miR167) upon V. dahliae infection downregulated the expression of their targeted genes (ARF10, NAC1, PHV and ARF6), respectively. In addition, we identified specific phased siRNAs generated from distinct regions of two libraries. Profiling of these miRNAs and sRNAs lay the foundation for further understanding and utilising the host-induced gene silencing strategy to control plant vascular pathogens.

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“…NAC domains from NAC1, AtNAM, and ANAC bind specifically to as-1 like sequence within the CaMV 35S promoter (Duval et al, 2002;Xie et al, 2000). Three dehydration-inducible NAC proteins (ANAC019, ANAC055, and ANAC072) bind specifically to the CATGTG motif of early response to dehydration 1 (ERD1) promoter both in vitro and in vivo (He et al, 2005).…”

Section: Discussionmentioning

confidence: 99%

“…The members of the NAC protein family are identifiable by the presence of a highly conserved N-terminal NAC domain accompanied by diverse C-terminal domains (Ernst et al, 2004). The DNA binding ability of this protein family has been assigned to the NAC domain (Duval et al, 2002;Ernst et al, 2004;Xie et al, 2000). Only a portion of the NAC genes has been studied to date and the family has been implicated in diverse processes (Fang et al, 2008).…”

Section: Introductionmentioning

confidence: 99%

ATAF2, a NAC Transcription Factor, Binds to the Promoter and Regulates NIT2 Gene Expression Involved in Auxin Biosynthesis

Huh

Lee

Kim

et al. 2012

Molecules and Cells

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The transcription factor ATAF2, one of the plant specific NAC family genes, is known as repressor of pathogenesisrelated genes and responsive to the diverse defense-related hormones, pathogen infection, and wounding stress. Furthermore, it is important to consider that tryptophandependant IAA biosynthesis pathway can be activated by wounding and pathogen. We found that ATAF2pro::GUS reporter was induced upon indole-3-acetonitrile (IAN) treatments. And ataf2 mutant showed reduced sensitivity to IAN whereas 35S::ATAF2 plants showed hyper-sensitivity to IAN. IAN biosynthesis required nitrilase involved in the conversion of IAN to an auxin, indole-3-acetic acid (IAA). We found that the NIT2 gene was repressed in ataf2 knockout plants. Expression of both ATAF2 and NIT2 genes was induced by IAN treatment. Transgenic plants overexpressing ATAF2 showed up-regulated NIT2 expression. ATAF2 activated promoter of the NIT2 gene in Arabidopsis protoplasts. Electrophoretic mobility shift assay revealed that NIT2 promoter region from position -117 to -82 contains an ATAF2 binding site where an imperfect palindrome sequence was critical to the protein-DNA interaction. These findings indicate that ATAF2 regulates NIT2 gene expression via NIT2 promoter binding.

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ArabidopsisNAC1 transduces auxin signal downstream of TIR1 to promote lateral root development

Cited by 834 publications

References 39 publications

Characterization of transcription factor gene SNAC2 conferring cold and salt tolerance in rice

Characterization of transcription factor gene SNAC2 conferring cold and salt tolerance in rice

Genome-wide profiling of sRNAs in the Verticillium dahliae-infected Arabidopsis roots

ATAF2, a NAC Transcription Factor, Binds to the Promoter and Regulates NIT2 Gene Expression Involved in Auxin Biosynthesis

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