<i>Arabidopsis</i>
            HEAT SHOCK FACTOR BINDING PROTEIN is required to limit meiotic crossovers and
            <i>HEI10</i>
            transcription

Kim, Juhyun; Park, Jihye; Kim, Heejin; Son, Namil; Kim, Eun‐Jung; Kim, Jaeil; Byun, Dohwan; Lee, Youngkyung; Park, Yeong Mi; Nageswaran, Divyashree C; Kuo, Pallas; Rose, Teresa; Dang, Tuong Vi T.; Hwang, Ildoo; Lambing, Christophe; Henderson, Ian; Choi, Kyuha

doi:10.15252/embj.2021109958

Cited by 10 publications

(3 citation statements)

References 75 publications

(155 reference statements)

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“…This method is based on extremely short interval lines (ESILs), which carry fluorescent reporters expressed in seeds, separated by no more than 50 kb. The fluorescently tagged intervals provide both precise CO frequency measurements 11,50,[52][53][54][55][56] and an easy assay for recombinants based on seed fluorescence. We used a recently developed large-scale collection of fluorescent reporter lines in Arabidopsis 57 to construct five ESILs by crossing the selected single-color reporter lines in the Col-0 background (Supplementary Fig.…”

Section: Construction Of Extremely Short Interval Lines Applicable To...mentioning

confidence: 99%

The effect of DNA polymorphisms and natural variation on crossover hotspot activity in Arabidopsis hybrids

et al. 2023

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In hybrid organisms, genetically divergent homologous chromosomes pair and recombine during meiosis; however, the effect of specific types of polymorphisms on crossover is poorly understood. Here, to analyze this in Arabidopsis, we develop the seed-typing method that enables the massively parallel fine-mapping of crossovers by sequencing. We show that structural variants, observed in one of the generated intervals, do not change crossover frequency unless they are located directly within crossover hotspots. Both natural and Cas9-induced deletions result in lower hotspot activity but are not compensated by increases in immediately adjacent hotspots. To examine the effect of single nucleotide polymorphisms on crossover formation, we analyze hotspot activity in mismatch detection-deficient msh2 mutants. Surprisingly, polymorphic hotspots show reduced activity in msh2. In lines where only the hotspot-containing interval is heterozygous, crossover numbers increase above those in the inbred (homozygous). We conclude that MSH2 shapes crossover distribution by stimulating hotspot activity at polymorphic regions.

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Section: Construction Of Extremely Short Interval Lines Applicable To...mentioning

confidence: 99%

The effect of DNA polymorphisms and natural variation on crossover hotspot activity in Arabidopsis hybrids

et al. 2023

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“…This coarsening model has been recently supported by experimental data showing that SC is critical for funneling chromosomal HEI10 distribution (Durand et al 2022) The dose-dependent effects of HEI10 on crossover number suggests the existence of factors that affect HEI10 expression and activity. A heat shock factor binding protein HSBP that regulates crossover number via controlling HEI10 transcription has already been identified (Kim et al 2022), and an additional protein functionally linked to HEI10, HEIP1, that participates in crossover regulation has been recently described in rice (Li et al 2018). Moreover, other regulatory circuits, such as that including HCR1, which encodes a protein phosphatase, were shown to have anti-recombination roles in the regulation of the ZMM pathway (Nageswaran et al 2021).…”

Section: Meiotic Crossover Control In Briefmentioning

confidence: 99%

Why do plants need the ZMM crossover pathway? A snapshot of meiotic recombination from the perspective of interhomolog polymorphism

Ziolkowski

2022

Plant Reprod

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At the heart of meiosis is crossover recombination, i.e., reciprocal exchange of chromosome fragments between parental genomes. Surprisingly, in most eukaryotes, including plants, several recombination pathways that can result in crossover event operate in parallel during meiosis. These pathways emerged independently in the course of evolution and perform separate functions, which directly translate into their roles in meiosis. The formation of one crossover per chromosome pair is required for proper chromosome segregation. This “obligate” crossover is ensured by the major crossover pathway in plants, and in many other eukaryotes, known as the ZMM pathway. The secondary pathways play important roles also in somatic cells and function mainly as repair mechanisms for DNA double-strand breaks (DSBs) not used for crossover formation. One of the consequences of the functional differences between ZMM and other DSB repair pathways is their distinct sensitivities to polymorphisms between homologous chromosomes. From a population genetics perspective, these differences may affect the maintenance of genetic variability. This might be of special importance when considering that a significant portion of plants uses inbreeding as a predominant reproductive strategy, which results in loss of interhomolog polymorphism. While we are still far from fully understanding the relationship between meiotic recombination pathways and genetic variation in populations, recent studies of crossovers in plants offer a new perspective.

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“…Heat stress affects various aspects of plant meiosis. In prophase I, elevated temperature alters the rate and distribution of meiotic recombination (10,12,13) and a recent study in Arabidopsis has shown that heat shock response pathway directly regulates the recombination machinery (14). More severe heat stress can impair synapsis and pairing of homologous chromosomes (15)(16)(17).…”

Section: Introductionmentioning

confidence: 99%

Heat stress impairs centromere structure and segregation of meiotic chromosomes in Arabidopsis

Khaitová

Mikulková

Pecinkova

et al. 2023

Preprint

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Heat stress is a major threat to global crop production, and understanding its impact on plant fertility is crucial for developing climate-resilient crops. Despite the known negative effects of heat stress on plant reproduction, the underlying molecular mechanisms remain poorly understood. Here, we investigated the impact of elevated temperature on centromere structure and chromosome segregation during meiosis in Arabidopsis thaliana. Our findings reveal that heat stress causes a significant decline in fertility and leads to the formation of micronuclei in pollen mother cells, along with an extended duration of meiotic division. We also demonstrate a reduction in the amounts of centromeric histone and the kinetochore protein BMF1 at meiotic centromeres with increasing temperature. Furthermore, we show that heat stress prolongs the activity of the spindle assembly checkpoint during meiosis I, indicating impaired efficiency of the kinetochore attachments to spindle microtubules. Our analysis of mutants with reduced amounts of centromeric histone suggests that weakened centromeres sensitize plants to elevated temperature, resulting in meiotic defects and reduced fertility even at moderate temperatures. These results indicate that the structure and functionality of meiotic centromeres in Arabidopsis are highly sensitive to heat stress, and suggest that centromeres and kinetochores may represent a crucial bottleneck in plant adaptation to increasing temperatures.

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Arabidopsis HEAT SHOCK FACTOR BINDING PROTEIN is required to limit meiotic crossovers and HEI10 transcription

Cited by 10 publications

References 75 publications

The effect of DNA polymorphisms and natural variation on crossover hotspot activity in Arabidopsis hybrids

The effect of DNA polymorphisms and natural variation on crossover hotspot activity in Arabidopsis hybrids

Why do plants need the ZMM crossover pathway? A snapshot of meiotic recombination from the perspective of interhomolog polymorphism

Heat stress impairs centromere structure and segregation of meiotic chromosomes in Arabidopsis

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