2022
DOI: 10.15252/embj.2021109958
|View full text |Cite
|
Sign up to set email alerts
|

Arabidopsis HEAT SHOCK FACTOR BINDING PROTEIN is required to limit meiotic crossovers and HEI10 transcription

Abstract: The number of meiotic crossovers is tightly controlled and most depend on pro‐crossover ZMM proteins, such as the E3 ligase HEI10. Despite the importance of HEI10 dosage for crossover formation, how HEI10 transcription is controlled remains unexplored. In a forward genetic screen using a fluorescent crossover reporter in Arabidopsis thaliana, we identify heat shock factor binding protein (HSBP) as a repressor of HEI10 transcription and crossover numbers. Using genome‐wide crossover mapping and cytogenetics, we… Show more

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
1
1
1

Citation Types

0
3
0

Year Published

2022
2022
2024
2024

Publication Types

Select...
4
1

Relationship

0
5

Authors

Journals

citations
Cited by 12 publications
(3 citation statements)
references
References 75 publications
0
3
0
Order By: Relevance
“…This method is based on extremely short interval lines (ESILs), which carry fluorescent reporters expressed in seeds, separated by no more than 50 kb. The fluorescently tagged intervals provide both precise CO frequency measurements 11,50,[52][53][54][55][56] and an easy assay for recombinants based on seed fluorescence. We used a recently developed large-scale collection of fluorescent reporter lines in Arabidopsis 57 to construct five ESILs by crossing the selected single-color reporter lines in the Col-0 background (Supplementary Fig.…”
Section: Construction Of Extremely Short Interval Lines Applicable To...mentioning
confidence: 99%
“…This method is based on extremely short interval lines (ESILs), which carry fluorescent reporters expressed in seeds, separated by no more than 50 kb. The fluorescently tagged intervals provide both precise CO frequency measurements 11,50,[52][53][54][55][56] and an easy assay for recombinants based on seed fluorescence. We used a recently developed large-scale collection of fluorescent reporter lines in Arabidopsis 57 to construct five ESILs by crossing the selected single-color reporter lines in the Col-0 background (Supplementary Fig.…”
Section: Construction Of Extremely Short Interval Lines Applicable To...mentioning
confidence: 99%
“…This coarsening model has been recently supported by experimental data showing that SC is critical for funneling chromosomal HEI10 distribution (Durand et al 2022) The dose-dependent effects of HEI10 on crossover number suggests the existence of factors that affect HEI10 expression and activity. A heat shock factor binding protein HSBP that regulates crossover number via controlling HEI10 transcription has already been identified (Kim et al 2022), and an additional protein functionally linked to HEI10, HEIP1, that participates in crossover regulation has been recently described in rice (Li et al 2018). Moreover, other regulatory circuits, such as that including HCR1, which encodes a protein phosphatase, were shown to have anti-recombination roles in the regulation of the ZMM pathway (Nageswaran et al 2021).…”
Section: Meiotic Crossover Control In Briefmentioning
confidence: 99%
“…Heat stress affects various aspects of plant meiosis. In prophase I, elevated temperature alters the rate and distribution of meiotic recombination (10,12,13) and a recent study in Arabidopsis has shown that heat shock response pathway directly regulates the recombination machinery (14). More severe heat stress can impair synapsis and pairing of homologous chromosomes (15)(16)(17).…”
Section: Introductionmentioning
confidence: 99%