<i>Arabidopsis</i>
            CLV3 Peptide Directly Binds CLV1 Ectodomain

Ogawa, Mari; Shinohara, Hidefumi; Sakagami, Youji; Matsubayashi, Yoshikatsu

doi:10.1126/science.1150083

Cited by 455 publications

(360 citation statements)

References 7 publications

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“…Intriguingly, the RxR motif is largely conserved in this subfamily of LRR-RKs ( Figure 1B). Furthermore, several members of this LRR-RK subfamily have been shown to recognize small peptides ending with histidine or asparagine [20][21][22][23][24][25]. These findings prompted us to hypothesize that members of the LRR XI subfamily may act as receptors of small peptides with a free C-terminal histidine or asparagine.…”

Section: Rationale For Matching Lrr-rlks With Their Ligandsmentioning

confidence: 99%

Signature motif-guided identification of receptors for peptide hormones essential for root meristem growth

et al. 2016

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Peptide-mediated cell-to-cell signaling has crucial roles in coordination and definition of cellular functions in plants. Peptide-receptor matching is important for understanding the mechanisms underlying peptide-mediated signaling. Here we report the structure-guided identification of root meristem growth factor (RGF) receptors important for plant development. An assay based on a signature ligand recognition motif (Arg-x-Arg) conserved in a subfamily of leucine-rich repeat receptor kinases (LRR-RKs) identified the functionally uncharacterized LRR-RK At4g26540 as a receptor of RGF1 (RGFR1). We further solved the crystal structure of RGF1 in complex with the LRR domain of RGFR1 at a resolution of 2.6 Å, which reveals that the Arg-x-Gly-Gly (RxGG) motif is responsible for specific recognition of the sulfate group of RGF1 by RGFR1. Based on the RxGG motif, we identified additional four RGFRs. Participation of the five RGFRs in RGF-induced signaling is supported by biochemical and genetic data. We also offer evidence showing that SERKs function as co-receptors for RGFs. Taken together, our study identifies RGF receptors and co-receptors that can link RGF signals with their downstream components and provides a proof of principle for structure-based matching of LRR-RKs with their peptide ligands.

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Section: Rationale For Matching Lrr-rlks With Their Ligandsmentioning

confidence: 99%

Signature motif-guided identification of receptors for peptide hormones essential for root meristem growth

et al. 2016

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“…To prepare HAR1 L246F -HT, the amino-acid substitution L246F was introduced into HAR1-HT by PCR-based site-directed mutagenesis. Transformation of tobacco BY-2 cells and preparation of microsomal fractions were described previously 36 . To confirm expression, HaloTag-fused proteins were specifically labelled using HaloTag TMR (Promega), separated by SDSpolyacrylamide gel electrophoresis, and visualized by a fluorescent image analyzer with a 523 nm excitation filter and a 580 nm emission filter.…”

Section: Methodsmentioning

confidence: 99%

Root-derived CLE glycopeptides control nodulation by direct binding to HAR1 receptor kinase

et al. 2013

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Leguminous plants establish a symbiosis with rhizobia to enable nitrogen fixation in root nodules under the control of the presumed root-to-shoot-to-root negative feedback called autoregulation of nodulation. In Lotus japonicus, autoregulation is mediated by CLE-RS genes that are specifically expressed in the root, and the receptor kinase HAR1 that functions in the shoot. However, the mature functional structures of CLE-RS gene products and the molecular nature of CLE-RS/HAR1 signalling governed by these spatially distant components remain elusive. Here we show that CLE-RS2 is a post-translationally arabinosylated glycopeptide derived from the CLE domain. Chemically synthesized CLE-RS glycopeptides cause significant suppression of nodulation and directly bind to HAR1 in an arabinose-chain and sequencedependent manner. In addition, CLE-RS2 glycopeptide specifically produced in the root is found in xylem sap collected from the shoot. We propose that CLE-RS glycopeptides are the long sought mobile signals responsible for the initial step of autoregulation of nodulation.

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“…The CLAVATA3 (CLV3)/EMBRYO SURROUNDING REGIONrelated (CLE) family of signalling peptides plays crucial roles in the regulation of stem cell maintenance in the meristems 3 . In the shoot apical meristem of Arabidopsis thaliana, CLV3 peptide secreted from stem cells restricts the expression domain of the WUSCHEL (WUS) homeobox transcription factor gene to the organizing centre via three receptor pathways, CLV1, CLV2-CORYNE (CRN) and RECEPTOR-LIKE PROTEIN KINASE 2 (RPK2) [4][5][6][7][8][9][10] . CLV3-WUS signalling makes a negative feedback loop to maintain the homoeostasis of stem cell population in the shoot apical meristem [11][12][13] .…”

mentioning

confidence: 99%

Plant GSK3 proteins regulate xylem cell differentiation downstream of TDIF–TDR signalling

et al. 2014

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Arabidopsis CLV3 Peptide Directly Binds CLV1 Ectodomain

Cited by 455 publications

References 7 publications

Signature motif-guided identification of receptors for peptide hormones essential for root meristem growth

Signature motif-guided identification of receptors for peptide hormones essential for root meristem growth

Root-derived CLE glycopeptides control nodulation by direct binding to HAR1 receptor kinase

Plant GSK3 proteins regulate xylem cell differentiation downstream of TDIF–TDR signalling

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