Apis andreniformis associated Actinomycetes show antimicrobial activity against black rot pathogen (Xanthomonas campestris pv. campestris)

Promnuan, Yaowanoot; Promsai, Saran; Pathom-aree, Wasu; Meelai, Sujinan

doi:10.7717/peerj.12097

Cited by 7 publications

(9 citation statements)

References 47 publications

(59 reference statements)

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“…Cacaoi, and S. puniceus are active against Xanthomonas campestris pv. campestris, the causal agent of the black rot disease (202). S. albidoflavus H12 and Nocardiopsis aegyptica H14 inhibit the growth of several bacterial phytopathogens, such as Pseudomonas syringae, P. corrugata, and Pectobacterium carotovorum subsp.…”

Section: Biocontrol Promoting Actinomycetes As Biopesticidesmentioning

confidence: 99%

The Potential Use of Actinomycetes as Microbial Inoculants and Biopesticides in Agriculture

et al. 2022

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The use of fertilizers and chemical pesticides promotes significant improvements in crop development, but some problems and risks associated with them limit their application. An alternative is using biological inputs based on microorganisms, increasing production while combining efficiency and sustainability. Actinomycetes are a group of bacteria belonging to the phylum Actinobacteria, recently re-named Actinobacteriota. They represent important microbial communities in the soils with increasing agricultural applications, especially in the biological control of insect-pest and plant disease and in plant growth promotion. Studies report their promising use as microbiological inoculants by exploring mechanisms to improve plant development, such as biological nitrogen fixation, phosphate solubilization, production of phytohormones, and other biocompounds. In addition, many species produce metabolic pathways that generate high-value antibiotics, extracellular enzymes and secondary metabolites other than antimicrobials, with potential in the control of phytopathogenic fungi, insects, and nematodes. These actinomycetes could be used to formulate novel bioinoculants composed of spores and/or mycelium. Considering that the research in this field is up-and-coming, with significant economic and environmental impacts in the future, this review aims to group the most relevant works that explore the biodiversity of actinomycetes, helping to develop inoculants and biodefensives for more productive and conscious agriculture.

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Section: Biocontrol Promoting Actinomycetes As Biopesticidesmentioning

confidence: 99%

The Potential Use of Actinomycetes as Microbial Inoculants and Biopesticides in Agriculture

et al. 2022

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“…(Anderson et al, 2011). Only very recently, diverse Streptomyces strains have been isolated from different bee species (Promnuan et al, 2021) and various parts of beehives, in particular from pollen stores (Kim et al, 2019;Grubbs et al, 2021). Most of these strains have the ability to inhibit pathogens of plants and bees (Kim et al, 2019;Grubbs et al, 2021), which could be beneficial for the health of these organisms.…”

Section: Introductionmentioning

confidence: 99%

Identification of Antimicrobial Compounds in Two Streptomyces sp. Strains Isolated From Beehives

et al. 2022

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The World Health Organization warns that the alarming increase in antibiotic resistant bacteria will lead to 2.7 million deaths annually due to the lack of effective antibiotic therapies. Clearly, there is an urgent need for short-term alternatives that help to alleviate these alarming figures. In this respect, the scientific community is exploring neglected ecological niches from which the prototypical antibiotic-producing bacteria Streptomycetes are expected to be present. Recent studies have reported that honeybees and their products carry Streptomyces species that possess strong antibacterial activity. In this study, we have investigated the antibiotic profile of two Streptomycetes strains that were isolated from beehives. One of the isolates is the strain Streptomyces albus AN1, which derives from pollen, and shows potent antimicrobial activity against Candida albicans. The other isolate is the strain Streptomyces griseoaurantiacus AD2, which was isolated from honey, and displays a broad range of antimicrobial activity against different Gram-positive bacteria, including pathogens such as Staphylococcus aureus and Enterococus faecalis. Cultures of S. griseoaurantiacus AD2 have the capacity to produce the antibacterial compounds undecylprodigiosin and manumycin, while those of S. albus AN1 accumulate antifungal compounds such as candicidins and antimycins. Furthermore, genome and dereplication analyses suggest that the number of putative bioactive metabolites produced by AD2 and AN1 is considerably high, including compounds with anti-microbial and anti-cancer properties. Our results postulate that beehives are a promising source for the discovery of novel bioactive compounds that might be of interest to the agri-food sector and healthcare pharmaceuticals.

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“…Bacteria are common bee-associated microorganisms and have also been the focus of attention (Zheng et al, 2018). In contrast to Gram-negative bacteria, Gram-positive Actinobacteria associated with bees are less well studied (Promnuan et al, 2021). Bee-associated Actinobacteria have been isolated from diverse bee species, including honeybees (Apis mellifera, A.…”

Section: Introductionmentioning

confidence: 99%

Diversity and antibacterial potential of the Actinobacteria associated with Apis mellifera ligustica

Cui

Jiang

et al. 2022

Front. Microbiol.

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Insect-associated Actinobacteria are a potentially rich source of novel natural products with antibacterial activity. Here, the community composition of Actinobacteria associated with Apis mellifera ligustica was investigated by integrated culture-dependent and independent methods. A total of 61 strains of Streptomyces genera were isolated from the honeycomb, larva, and different anatomical parts of the honeybee’s body using the culture-dependent method. Amplicon sequencing analyses revealed that the actinobacterial communities were dominated by the family of Bifidobacteriaceae and Microbacteriaceae in the honeybee gut, and Nocardiaceae and Pseudonocardiaceae in the honeycomb, whereas only Streptomyces genera were isolated by the culture-dependent method. Culture-independent analyses showed more diverse actinobacterial communities than those of culture-dependent methods. The antibacterial bioassay showed that most crude extracts of representative isolates exhibited antibacterial activities. Among them, the crude extract of Streptomyces sp. FCF01 showed the best antibacterial activities against Staphylococcus aureus, Micrococcus tetragenus, and Pseudomonas syringae pv. actinidiae (Psa) with the disc diameter of inhibition zone diameter (IZD) of 23.00, 15.00, and 13.33 mm, respectively. Chemical analysis of Streptomyces sp. FCF01 led to the isolation of three secondary metabolites, including mayamycin (1), mayamycin B (2), and N-(2-Hydroxyphenyl) acetamide (3). Among them, compound 1 displayed strong antibacterial activity against S. aureus, M. tetragenus, and Psa with minimum inhibitory concentrations (MIC) values of 6.25, 12.5, and 6.25 μg/ml, respectively. In addition, two novel derivative compounds 1a and 1b were synthesized by acetylation of compound 1. Both compounds 1a and 1b displayed similar antibacterial activities with those of metabolite 1. These results indicated that Streptomyces species associated with honeybees had great potential in finding antibiotics.

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Apis andreniformis associated Actinomycetes show antimicrobial activity against black rot pathogen (Xanthomonas campestris pv. campestris)

Cited by 7 publications

References 47 publications

The Potential Use of Actinomycetes as Microbial Inoculants and Biopesticides in Agriculture

The Potential Use of Actinomycetes as Microbial Inoculants and Biopesticides in Agriculture

Identification of Antimicrobial Compounds in Two Streptomyces sp. Strains Isolated From Beehives

Diversity and antibacterial potential of the Actinobacteria associated with Apis mellifera ligustica

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