<i>AOX</i>
            gene diversity in arbuscular mycorrhizal fungi (AMF) products

Mercy, Louis; Svensson, Jan T.; Lucic, Eva; Cardoso, Hélia; Nogales, Amaia; Döring, Matthias; Jurgeleit, Jens; Schneider, Caroline; Arnholdt‐Schmitt, Birgit

doi:10.1002/9781118789971.ch20

Cited by 5 publications

(5 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Furthermore, it was found that AOX polymorphisms could be used to distinguish individual plants from the same species (101). Symbiotic AMF revealed substantial AOX gene polymorphism within and between spores (102), an observation which awaits to be functionally explored in relation to adaptive plant holobiont performance (103)(104)(105)(106)(107).…”

Section: Resultsmentioning

confidence: 99%

ROS/RNS Balancing, Aerobic Fermentation Regulation and Cell Cycle Control – a Complex Early Trait (‘CoV-MAC-TED’) for Combating SARS-CoV-2-Induced Cell Reprogramming

et al. 2021

Self Cite

View full text Add to dashboard Cite

In a perspective entitled ‘From plant survival under severe stress to anti-viral human defense’ we raised and justified the hypothesis that transcript level profiles of justified target genes established from in vitro somatic embryogenesis (SE) induction in plants as a reference compared to virus-induced profiles can identify differential virus signatures that link to harmful reprogramming. A standard profile of selected genes named ‘ReprogVirus’ was proposed for in vitro-scanning of early virus-induced reprogramming in critical primary infected cells/tissues as target trait. For data collection, the ‘ReprogVirus platform’ was initiated. This initiative aims to identify in a common effort across scientific boundaries critical virus footprints from diverse virus origins and variants as a basis for anti-viral strategy design. This approach is open for validation and extension. In the present study, we initiated validation by experimental transcriptome data available in public domain combined with advancing plant wet lab research. We compared plant-adapted transcriptomes according to ‘RegroVirus’ complemented by alternative oxidase (AOX) genes during de novo programming under SE-inducing conditions with in vitro corona virus-induced transcriptome profiles. This approach enabled identifying a major complex trait for early de novo programming during SARS-CoV-2 infection, called ‘CoV-MAC-TED’. It consists of unbalanced ROS/RNS levels, which are connected to increased aerobic fermentation that links to alpha-tubulin-based cell restructuration and progression of cell cycle. We conclude that anti-viral/anti-SARS-CoV-2 strategies need to rigorously target ‘CoV-MAC-TED’ in primary infected nose and mouth cells through prophylactic and very early therapeutic strategies. We also discuss potential strategies in the view of the beneficial role of AOX for resilient behavior in plants. Furthermore, following the general observation that ROS/RNS equilibration/redox homeostasis is of utmost importance at the very beginning of viral infection, we highlight that ‘de-stressing’ disease and social handling should be seen as essential part of anti-viral/anti-SARS-CoV-2 strategies.

show abstract

Section: Resultsmentioning

confidence: 99%

ROS/RNS Balancing, Aerobic Fermentation Regulation and Cell Cycle Control – a Complex Early Trait (‘CoV-MAC-TED’) for Combating SARS-CoV-2-Induced Cell Reprogramming

et al. 2021

Self Cite

View full text Add to dashboard Cite

show abstract

“…Based on the role of AOX in carbohydrate metabolism (Vanlerberghe et al, 1994), our approach has been stimulated to understand the role of fermentation and sugars during plant-mycorrhiza interactions (Mercy et al, 2017;Bedini et al, 2018) and had led to a privately explored patent (Lucic and Mercy, 2014). However, the early phase of reprogramming was not sufficiently considered in that research (Mercy et al, 2017) to drive our core functional marker approach (Arnholdt-Schmitt, 2008;Mercy et al, 2015). Recently, Mohanapriya et al (2019) observed that arbuscular mycorrhizal fungi (AMF) inoculation in carrot seeds interacted with the AOX-inhibitor salicyl hydroxamic acid (SHAM) and palliated negative SHAM effects on early germination.…”

Section: Aox Is a Key Molecule For Cellular Reprogramming: Toward A Perspectivementioning

confidence: 99%

“…Understanding the role of microbiota in adaptive plant robustness is important for crop improvement and developing innovative tools that could allow more efficient plant selection (Arnholdt-Schmitt et al, 2014;Arnholdt-Schmitt et al, 2015Nogales et al, 2015). Research on the relevance of endophytic and associated microbiota and usage of microbes as bioinoculants are often hampered by low reproducibility, due to a lack of better understanding of fundamental principles of the plant-microbe interactions (Arnholdt-Schmitt, 2008;Vicente and Arnholdt-Schmitt, 2008;Campos et al, 2015;Mercy et al, 2015Mercy et al, , 2017Bedini et al, 2018;Albornoz et al, 2020) emphasized the need to study mycorrhizal benefits on a case-by-case basis which should consider more holistic and context-dependent views on mycorrhiza functioning at plant family and biomewide levels. Also, it is widely confirmed that endophyte effects are genotype-specific (Abdelrazek et al, 2020a,b).…”

Section: Introductionmentioning

confidence: 99%

Adaptive Reprogramming During Early Seed Germination Requires Temporarily Enhanced Fermentation-A Critical Role for Alternative Oxidase Regulation That Concerns Also Microbiota Effectiveness

et al. 2021

Self Cite

View full text Add to dashboard Cite

Plants respond to environmental cues via adaptive cell reprogramming that can affect whole plant and ecosystem functionality. Microbiota constitutes part of the inner and outer environment of the plant. This Umwelt underlies steady dynamics, due to complex local and global biotic and abiotic changes. Hence, adaptive plant holobiont responses are crucial for continuous metabolic adjustment at the systems level. Plants require oxygen-dependent respiration for energy-dependent adaptive morphology, such as germination, root and shoot growth, and formation of adventitious, clonal, and reproductive organs, fruits, and seeds. Fermentative paths can help in acclimation and, to our view, the role of alternative oxidase (AOX) in coordinating complex metabolic and physiological adjustments is underestimated. Cellular levels of sucrose are an important sensor of environmental stress. We explored the role of exogenous sucrose and its interplay with AOX during early seed germination. We found that sucrose-dependent initiation of fermentation during the first 12 h after imbibition (HAI) was beneficial to germination. However, parallel upregulated AOX expression was essential to control negative effects by prolonged sucrose treatment. Early downregulated AOX activity until 12 HAI improved germination efficiency in the absence of sucrose but suppressed early germination in its presence. The results also suggest that seeds inoculated with arbuscular mycorrhizal fungi (AMF) can buffer sucrose stress during germination to restore normal respiration more efficiently. Following this approach, we propose a simple method to identify organic seeds and low-cost on-farm perspectives for early identifying disease tolerance, predicting plant holobiont behavior, and improving germination. Furthermore, the research strengthens the view that AOX can serve as a powerful functional marker source for seed hologenomes.

show abstract

“…Understanding the role of microbiota in adaptive plant robustness is important for crop improvement and for developing innovative tools that could allow more efficient plant selection (Arnholdt-Schmitt et al, 2014, Nogales et al, 2015, Arnholdt-Schmitt et al, 2015, Arnholdt-Schmitt et al, 2018). Research on the relevance of endophytic and associated microbiota and usage of microbiota inoculation are often hampered by low reproducibility, which is due to a lack of better understanding the fundamental principles of functional plant-microbiota interaction (Arnholdt-Schmitt, 2008, Vicente and Arnholdt-Schmitt, 2008, Mercy et al, 2015, Campos et al, 2015, Mercy et al, 2017, Bedini et al, 2018). Albornoz et al (2020) emphasizes the need for studying mycorrhizal benefits on a case-by-case basis that should consider more holistic and context-dependent views on mycorrhiza functioning at plant family- and biome-wide levels.…”

Section: Introductionmentioning

confidence: 99%

“…Based on the role of AOX in carbohydrate metabolism (Vanlerberghe et al, 1994), our approach stimulated reflecting on the role of fermentation and sugars for plant –mycorrhiza interaction (Mercy et al, 2017, Bedini et al, 2018) and had led to a privately explored patent (Mercy and Mercy, 2014). However, the early phase of reprogramming was not sufficiently considered in that research (Mercy et al, 2017) to drive our core functional marker approach (Arnholdt-Schmitt, 2008, Mercy et al, 2015). Recently, Mohanapriya et al (2019) observed that AMF inoculation in imbibed seeds interacted with the AOX-inhibitor SHAM and palliated negative SHAM effects on early germination.…”

Section: Introductionmentioning

confidence: 99%

Adaptive reprogramming during early seed germination requires temporarily enhanced fermentation – a critical role for alternative oxidase (AOX) regulation that concerns also microbiota effectiveness

Bharadwaj

Noceda

Mohanapriya

et al. 2021

Preprint

Self Cite

View full text Add to dashboard Cite

Plants respond to environmental cues via adaptive cell reprogramming that can affect whole plant and ecosystem functionality. Microbiota constitutes part of plants inner and outer environment. This Umwelt underlies steady dynamics, due to complex local and global biotic and abiotic changes. Hence, adaptive plant holobiont responses are crucial for continuous metabolic adjustment at systems levels. Plants require oxygen-dependent respiration for energy-dependent adaptive morphology, such as, germination, root and shoot growth, formation of adventitious, clonal and reproductive organs, fruits and seeds. Fermentative paths can help in acclimation and, to our view the role of alternative oxidase (AOX) in coordinating complex metabolic and physiologic adjustments is underestimated. Cellular level of sucrose is an important sensor of environmental stress. We explored the role of exogenous sucrose and its interplay with AOX during early seed germination. We found that sucrose-dependent initiation of fermentation during the first 12 hours after imbibition (HAI) was beneficial to germination. However, parallel enhanced AOX expression was essential to control negative effects by prolonged sucrose treatment. Early down-regulated AOX activity until 12 HAI improved germination efficiency in the absence of sucrose, but suppressed early germination in its presence. Our results also suggest that seeds-inoculated arbuscular mycorrhizal fungi can buffer sucrose stress during germination to restore normal respiration more efficiently. Following this approach, we propose a simple method to identify organic seeds and low-cost on-farm perspectives for early selection on disease tolerance, predicting plant holobiont behavior and improving germination. Furthermore, our research strengthens the view that AOX can serve as powerful functional marker source for seed hologenomes.

show abstract

AOX gene diversity in arbuscular mycorrhizal fungi (AMF) products

Cited by 5 publications

References 0 publications

ROS/RNS Balancing, Aerobic Fermentation Regulation and Cell Cycle Control – a Complex Early Trait (‘CoV-MAC-TED’) for Combating SARS-CoV-2-Induced Cell Reprogramming

ROS/RNS Balancing, Aerobic Fermentation Regulation and Cell Cycle Control – a Complex Early Trait (‘CoV-MAC-TED’) for Combating SARS-CoV-2-Induced Cell Reprogramming

Adaptive Reprogramming During Early Seed Germination Requires Temporarily Enhanced Fermentation-A Critical Role for Alternative Oxidase Regulation That Concerns Also Microbiota Effectiveness

Adaptive reprogramming during early seed germination requires temporarily enhanced fermentation – a critical role for alternative oxidase (AOX) regulation that concerns also microbiota effectiveness

Contact Info

Product

Resources

About