Angelica gigas Nakai Has Synergetic Effects on Doxorubicin-Induced Apoptosis

Abstract

Natural products are valuable sources for drug discovery because they have a wide variety of useful chemical components and biological properties. A quick reevaluation of the potential therapeutic properties of established natural products was made possible by the recent development of the methodology and improvement in the accuracy of an automated high-throughput screening system. In this study, we screened natural product libraries to detect compounds with anticancer effects using HeLa cells. Of the 420 plant extracts screened, the extract of Angelica gigas Nakai (AGN) was the most effective in reducing cell viability of HeLa cells. Markers of apoptosis, such as exposure of phosphatidylserine and cleavage of caspase-7 and PARP, were increased by treatment with the AGN extract. Treatment of the AGN extract increased expression of PKR as well as ATF4 and CHOP, the unfolded protein response genes. In addition, cotreatment of doxorubicin and the AGN extract significantly increased doxorubicin-induced apoptosis in HeLa cells. Decursin and decursinol angelate, which were known to have anticancer effects, were the main components of the AGN extract. These results suggest that the extract of AGN containing, decursin and decursinol angelate, increases doxorubicin susceptibility.

Effect of Angelica gigas Nakai Ethanol Extract and Decursin on Human Pancreatic Cancer Cells

“…The results showed that decursin is the main constituent of AGE. According to a previous study, the second peak in the UPLC assay may indicate decursinol angelate [8]. In this study, AGE and decursin showed growth inhibition and colony formation of PANC-1 and MIA PaCa-2 cells in a dose-dependent manner.…”

“…It has been reported that the extract of Angelica gigas Nakai contains decursin [8]. We first confirmed the presence of decursin in AGE by ultra-performance liquid chromatography (UPLC) assay conducted using AGE and decursin under the same conditions.…”

“…It has been applied to improve poor circulation, blood deficiency, and gynecologic diseases in East Asia. Decursin, which is one of the effective compounds of Angelica gigas Nakai, has diverse biological activities [8]. Angelica gigas Nakai and decursin have various pharmacological effects, such as anti-inflammatory, antiosteoclastic, and anticancer effects [9][10][11].…”

Levosimendan Protects against Doxorubicin-Induced Cardiotoxicity by Regulating the PTEN/Akt Pathway

“…Apoptosis has been shown to be one of the key processes in DOX-induced cardiac injury [4,8,23,24]. We observed few apoptotic cells in the CON and CON+L groups, but more apoptotic cells in the DOX group.…”

“…The cumulative and dose-dependent toxicity induced by DOX is harmful to nontumor tissues, and in myocardial tissue, DOX causes irreversible damage, which can lead to dilated cardiomyopathy, greatly limiting its clinical application [4,5]. Many mechanisms underlying DOX-induced cardiotoxicity have been discovered, including mitochondrial iron accumulation and related redox reactions, the activation of immunological reactions, histamine release, and DNA damage, and emerging research indicates a crucial role for apoptosis [6][7][8].…”