Andrographis paniculataameliorates carbon tetrachloride (CCl₄)-dependent hepatic damage and toxicity: Diminution of oxidative stress

Abstract

Andrographis paniculata (hempedu bumi) is a plant that possesses many medicinal values in treating several diseases and for health care maintenance. However, its hepatoprotective activity and mechanism of action have not been fully investigated. Therefore, this study aimed to evaluate the hepatoprotective effects of A. paniculata and its mechanism of action in rats. Carbon tetrachloride (CCl(4)) challenge of rats at a dose of 1.2 ml/kg body weight-induced oxidative stress in the liver. This was evidenced by augmentation in lipid peroxidation, which was accompanied by a decrease in the activities of antioxidant enzymes and depletion in the level of reduced glutathione (P < 0.05). Parrallel to these changes, CCl(4) challenge too, enhanced hepatic damage as evidenced by sharp increase in serum transaminases (e.g. alanine aminotransferase, aspartate aminotransferase, and lactate dehydrogenase) (P < 0.05). Additionally, the impairment of liver function corresponded to histolopathological changes. However, most of these changes were reversed in a dose-dependent fashion by pre-treatment of animals with A. paniculata (P < 0.05). The ability of A. paniculata to scavenge the 2,2-Diphenyl-2-picrylhydrazyl radical was determined through its EC(50) value. The EC(50) value of A. paniculata was 583.60 ± 4.25 µg/ml. In addition, A. paniculata was found to contain 65.37 ± 1.20 mg/g total phenolics expressed as gallic acid equivalent. From these studies, it is concluded that A. paniculata could be used as a hepatoprotective agent and possesses the potential to treat or prevent degenerative diseases where oxidative stress is implicated.

Thành phần hóa học, khả năng kháng khuẩn và kháng oxi hóa của Xuyên tâm liên Andrographis paniculata (Burm.f.) nees phân bố ở Cần Thơ, Sóc Trăng và An Giang

“…Ngược lại cao chiết ethanol ở Sóc Trăng cho thấy khả năng kháng oxi hóa là yếu nhất, cần một lượng 388,21 ± 15,84 µg/mL cao chiết để trung hòa 50% gốc tự do. Khi so sánh với nghiên cứu của (Koh et al, 2011) về khả năng kháng oxi hóa của A. paniculata cho thấy giá trị EC 50 = 583,60 ± 4,25 𝜇g/mL thể hiện hoạt tính kháng oxi hóa yếu hơn tất cả các cao chiết của A. paniculata trong nghiên cứu này. Từ đó có thể thấy rằng, các kết quả kháng oxi hóa ở trong nghiên cứu này thể hiện hoạt tính kháng oxi hóa của A. paniculata phụ thuộc vào các hợp chất thứ cấp được tích lũy khi được trồng ở các vùng địa lý, sinh cảnh khác nhau và loại dung môi dùng để chiết cao.…”

Effects of Andrographis paniculata on Carbon Tetrachloride (CCl4)-Mediated Renal Oxidative Damage in Rats

“…These samples were then used for biochemical and histopathological analyses to evaluate renal function impairment. Doses of A. paniculata were selected based on previously published data (Koh et al, 2011).…”

Hepatoprotective Potential of Malaysian Medicinal Plants: A Review on Phytochemicals, Oxidative Stress, and Antioxidant Mechanisms

“…The activities of CAT, GPX, QR, GST, GR, and G6PD in the hepatic tissues were significantly restored by 22 to 94%. Further, the exposure of the solvent extract of the plant reduced degenerative changes such as fatty changes, cellular hypertrophy, necrotic cells, inflammatory cell infiltration, and sinusoidal dilatation induced by CCl 4 administration [ 84 ].…”