Anaplasma phagocytophilumfrom Rodents and Sheep, China

Abstract

Three strains were isolated and characterized.

Rural Residents in China Are at Increased Risk of Exposure to Tick-Borne PathogensAnaplasma phagocytophilumandEhrlichia chaffeensis

“…Among these HGA isolates, two human isolates and one tick isolate from Shandong Peninsula, where all of the patients exhibited severe clinical manifestations, were identical to each other, based on an analysis of 16S rRNA and the ankA and msp2   genes but were different in sequence from sequences identified in other parts of the world. Moreover, the 16S rRNA gene of the five Chinese HGA isolates showed 99% identity with the strain China-C-Tt (GQ 412339) in Tscherskia triton , the strain China-C-Y (GQ412338) in domestic sheep, and the strain China-C-Aa (GQ 412337) in Apodemus agrarius from the northeastern areas of China [4]. Here, we had to address that some genetic groups of A. phagocytophilum identified in China were related to human infection, while others might be only associated with sylvatic or domestic animals but not able to infect humans.…”

“…Both bacteria are transmitted by hard ticks, and certain wild rodents and animals are reservoirs. In China, A. phagocytophilum bacteria had been isolated from Apodemus agrarius, Tscherskia triton, and sheep, respectively, and these animals might be reservoirs hosts of A. phagocytophilum [4]. Moreover, a recent national investigation assessing the epidemiological status of A. phagocytophilum among domestic animals in 10 provinces/cities in China showed that some domestic animals including dogs, goats, and cattle might be important reservoirs hosts of A. phagocytophilum [5].…”

“…Despite serological, molecular, and even etiological evidence demonstrating the nationwide distribution of A. phagocytophilum infections in humans, domestic animals, ticks, and rodents [4, 12–19], large-scale laboratory-based serological investigations among rural residents who may be at an increased risk of occupational and residential exposure are limited. Thus, it is crucial to obtain epidemiological data on geographical, occupational, and residential risk factors that could increase disease exposure.…”

Molecular characterization and specific detection of Anaplasma species (AP-sd) in sika deer and its first detection in wild brown bears and rodents in Hokkaido, Japan

“…Therefore, the bacterial morphology of AP-sd is needed to elucidate its taxonomic position and physiological needs. A. phagocytophilum strains are able to grow in HL-60 and ISE6 cell lines (Massung et al, 2006a;Zhan et al, 2010). However, A. bovis, which infects some mammalian monocytes, has not been isolated in vitro.…”

Detection of Anaplasma sp. phylogenetically related to A. phagocytophilum in a free-living bird in Brazil

“…Several reports highlighted the participation of wild mammals, such as deer, foxes, rodents, and carnivores in A. phagocytophilum cycles, including their role as hosts of these bacteria (LIZ et al, 2000;FUENTE et al, 2008;VERONESI et al, 2011;ANDRÉ et al, 2012, JAHFARI et al, 2014. Wild and domestic animals may share pathogens, as described by Zhan et al (2010), who found goats, sheep, and wild rodents in the same area infected by the same strain of A. phagocytophilum. Such occurrences can bring the agent in closer contact with humans.…”