<i>Anandenanthera colubrina</i> (Vell.) Brenan as an inhibitor of HIV-1 BaL infection

Maia, Carolina Medeiros de Almeida; Pasetto, Silvana; Silva, Joanda Paolla Raimundo e; Tavares, Josean Fechine; Costa, Edja Maria Melo de Brito; Murata, Ramiro Mendonça

doi:10.1080/14786419.2021.1892097

Cited by 6 publications

(6 citation statements)

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“…The quality of the botanical material, as well as the adequate processing of the fresh material, including drying, transportation, storage, and the use of appropriate and reproducible extraction techniques have a straight outcome on health benefits and economic issues. Previous studies were conducted to determine the best conditions for A. colubrina extract preparation ( Lima et al, 2014 ; Rocha et al, 2017 ; Lima et al, 2018– data not published ; Maia et al, 2021 ) The presence of polyphenol content of 53.1% ( Lima et al, 2014 ) and 53.18% ( Rocha et al, 2017 ) gallic acid equivalents; tannins (8.7% catechin equivalents) and flavonoids (0.3% quercetin equivalents) ( Lima et al, 2014 ), compounds with straight relationship with A. colubrina extract activity were also recently monitored by GC-MS analysis ( Maia et al, 2021 ). These results, presented in Table 1 , suggest the maintenance of the same phytochemical profile of the standardized extract.…”

Section: Resultsmentioning

confidence: 99%

“…Recently, the traditional therapeutic use of A. colubrina by popular communities in Brazilian culture has encouraged comprehensive studies about its biological properties to be conducted ( Damascena et al, 2014 ; Lima et al, 2014 ; Barreto et al, 2016 ; Mota et al, 2017 ; Guarneire et al, 2019 ; Silva et al, 2019 ; Cardoso-Junior et al, 2020 ; Maia et al, 2021 ). In this study, we evaluated the A. colubrina extract effects on virulence factor of C. albicans and its modulatory effects on host immune response during the fungal infection.…”

Section: Discussionmentioning

confidence: 99%

“…Considering the same standardized extract (same batch of plant material and same extraction process) used in this research and used by Lima et al (2014) , Rocha et al (2017) , Lima et al (2018– data not published ) and Silva et al (2019) , the presence of polyphenol, tannins and flavonoids, compounds with straight relationship with A. colubrina extract pharmacological activity, were also recently monitored by gas chromatograph (GC) coupled to mass spectrometer (MS) with electron impact ionization (EI) (model GCMS-QP2010 Ultra, Shimadzu) according to the method described by Maia et al (2021) , using the same standardized material.…”

Section: Methodsmentioning

confidence: 99%

“…It is a woody species typical of the Caatinga Brazilian biome ( Silva et al, 2019 ) and its use by the traditional communities of Brazilian semiarid as a medicinal plant is common, which includes the treatment of inflammation in general ( Araújo et al, 2014 ; Araújo et al, 2015 ). Recent researches have shown that A. colubrina has promising therapeutic properties, such as antifungal ( Lima et al, 2014 ; Silva et al, 2019 ), anti-proliferative ( Lima et al, 2014 ), anti-inflammatory ( Guarneire et al, 2019 ; Cardoso-Junior et al, 2020 ), antioxidant ( Araújo et al, 2019 ; Cardoso-Junior et al, 2020 ), and anti-HIV ( Maia et al, 2021 ).…”

Section: Introductionmentioning

confidence: 99%

“…Phytochemical compounds identified in the hydroalcoholic bark extract of A. colubrina by GC-MS(Maia et al, 2021).…”

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confidence: 99%

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Yeast-Host Interactions: Anadenanthera colubrina Modulates Virulence Factors of C. albicans and Inflammatory Response In Vitro

et al. 2021

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Oral candidiasis is one of the most common fungal infections in humans. Its incidence has increased widely, as well as the antifungal resistance, demanding for the search for novel antifungal therapeutic agents. Anadenanthera colubrina (Vell.) Brenan is a plant species that has been proven to possess pharmacological effects, including antifungal and anti-inflammatory activities. This study evaluated in vitro the effects of standardized A. colubrina extract on virulence factors of Candida albicans and its regulation on immune response through C. albicans-host interaction. Antifungal activity was evaluated by Broth Microdilution Method against reference Candida strains (C. albicans, C. glabrata, C. tropicalis; C. dubliniensis). Anti-biofilm effect was performed on C. albicans mature biofilm and quantified by CFU/mL/g of biofilm dry weight. Proleotlytic enzymatic activities of proteinase and phospholipase were assessed by Azocasein and Phosphatidylcholine assays, respectively. Cytotoxicity effect was determined by Cell Titer Blue Viability Assay on Human Gingival Fibroblasts. Co-cultured model was used to analyze C. albicans coexisting with HGF by Scanning Electron Microscopy and fluorescence microscopies; gene expression was assessed by RT-PCR of C. albicans enzymes (SAP-1, PLB-1) and of host inflammatory cytokines (IL-6, IL-8, IL-1β, IL-10). Cytokines secretion was analysed by Luminex. The extract presented antifungal effect with MIC<15.62 μg/ml against Candida strains. Biofilm and proteolytic activity were significant reduced at 312.4 μg/ml (20 × 15.62 μg/ml) extract concentration. Cell viability was maintained higher than 70% in concentrations up to 250 μg/ml (LD50 = 423.3 μg/ml). Co-culture microscopies demonstrated a substantial decreased in C. albicans growth and minimal toxicity against host cells. Gene expressions of SAP-1/PLB-1 were significantly down-regulated and host immune response was modulated by a significant decreased on IL-6 and IL-8 cytokines secretion. A. colubrina had antifungal activity on Candida strains, antibiofilm, and anti-proteolytic enzyme effects against C. albicans. Presented low cytotoxicity to the host cells and modulatory effects on the host immune response.

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