Alternanthera mosaic potexvirus: Several Features, Properties, and Application

Abstract

Alternanthera mosaic virus (AltMV) is a typical member of the Potexvirus genus in its morphology and genome structure; still it exhibits a number of unique features. They allow this virus to be considered a promising object for biotechnology. Virions and virus-like particles (VLPs) of AltMV are stable in a wide range of conditions, including sera of laboratory animals. AltMV VLPs can assemble at various pH and ionic strengths. Furthermore, AltMV virions and VLPs demonstrate high immunogenicity, enhancing the immune response to the target antigen thus offering the possibility of being used as potential adjuvants. Recently, for the first time for plant viruses, we showed the structural difference between morphologically similar viral and virus-like particles on AltMV virions and VLPs. In this review, we discuss the features of AltMV virions, AltMV VLP assembly, and their structure and properties, as well as the characteristics of AltMV isolates, host plants, infection symptoms, AltMV isolation and purification, genome structure, viral proteins, and AltMV-based vectors.

A Recombinant Rotavirus Antigen Based on the Coat Protein of Alternanthera Mosaic Virus

“…To enhance the immunogenicity of this epitope, we selected the coat protein of the plant virus AltMV (strain MU) as a carrier. According to the data obtained in our previous work, both AltMV virions and VLPs exhibit adjuvant properties [9], which inspired hope that VLPs or RNPs formed with AltMV CP could efficiently enhance the immune response to the epitope of rotavirus A protein VP6. Based on the study by Tremblay et al, who showed that in VLPs of .…”

“…In particular, it seems promising to obtain VLPs based on coat proteins of plant viruses, which are safe for humans, possess strong immunostimulating properties, and can be obtained cost-effectively [8]. One plant virus with immunostimulating properties is Alternanthera mosaic virus (AltMV) [9]. AltMV belongs to the Potexvirus genus of the Alphaflexiviridae family.…”

Surface Charge Mapping on Virions and Virus-Like Particles of Helical Plant Viruses

“…At the same time, the assembly of PVX virions begins directly at the 5'end of the molecule; RNA interacts with CP monomers and dimers [27]. There is little data on the assembly of AltMV, but the assembly apparently occurs in the same way as in PVX [29]. It should be noted that in TMV, PVX, and AltMV, despite the fact that the assembly of virions occurs in different scenarios, the surface charge is distributed unevenly and an increased negative charge density area is located at one end of the virion.…”

Новый Подход К Разработке Кандидатной Вакцины Против Ротавируса На Основе Двух Вирусов Растений

“…Для вирионов и ВПЧ вируса мозаики альтернантеры (ВМАльт) (Alternanthera mosaic virus, род Potexvirus, сем. Alphaflexiviridae) ранее были продемонстрированы адъювантные свойства, а белок оболочки (БО) ВМАльт обладает способностью образовывать не содержащие РНК нитевидные ВПЧ, стабильные в физиологических условиях (35). В своих предыдущих исследованиях мы использовали БО ВМАльт в качестве носителя для эпитопа RV14, в результате чего был получен химерный рекомбинантный белок ER6 (Epitope of Rotavirus protein VP6) с молекулярной массой 25 кДа (36).…”