<i>Agrobacterium vitis</i>Strain AB3 Harbors Two Independent Tartrate Utilization Systems, One of Which Is Encoded by theTi Plasmid

Otten, Léon; Crouzet, Philippe; Salomone, J.-Y.; Ruffray, Patrice de; Szegedi, E.

doi:10.1094/mpmi-8-0138

Cited by 22 publications

(21 citation statements)

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“…The 230-kb plasmid is considered to be the tumorinducing plasmid due to the presence of virB gene. This notion is supported by the plasmid's size similarity to Ti and Ri plasmids such as pTiAB3 (Otten et al, 1995).…”

Section: Discussionmentioning

confidence: 98%

Construction of physical map and mapping of chromosomal virulence genes of the biovar 3 Agrobacterium (Rhizobium vitis) strain K-Ag-1

et al. 2006

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Section: Discussionmentioning

confidence: 98%

Construction of physical map and mapping of chromosomal virulence genes of the biovar 3 Agrobacterium (Rhizobium vitis) strain K-Ag-1

et al. 2006

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“…For mutagenesis and functional tests, a 6.2-kb fragment of the pBin19 derivative pPM4507 encoding tartrate utilization (Fig. 1A) was recloned into the broad-host-range vector pMP2733 (16,31). To this end, a 7.1-kb KpnI-XbaI DNA fragment of pPM4507 was cloned in the general cloning vector pUC18.…”

Section: Methodsmentioning

confidence: 99%

“…In planta mating experiments (31,48) have shown that in several A. vitis strains the tartrate degradation genes are carried by conjugative plasmids. The tartrate utilization plasmid of the A. vitis nopaline strain AB4 (pTrAB4) was chosen for further studies because of its relatively small size (170 kb).…”

mentioning

confidence: 99%

Sequence and mutational analysis of a tartrate utilization operon from Agrobacterium vitis

Crouzet¹,

Otten²

1995

J Bacteriol

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The grapevine is the natural host of the tumorigenic bacterium Agrobacterium vitis. Most of the A. vitis isolates can use tartrate, an unusually abundant compound in grapevine. The nopaline strain, AB4, contains a 170-kb conjugative plasmid (pTrAB4) encoding tartrate utilization. A 5.65-kb pTrAB4 region which enables non-tartrate-utilizing Agrobacterium tumefaciens to grow on tartrate was sequenced and mutagenized with the transcriptional fusion transposon Tn5-uidA1. This DNA fragment contains four intact open reading frames (ORFs) (ttuABCD) required for tartrate-dependent growth. The mutant phenotypes of each ORF, their homologies to published sequences, and their induction patterns allowed us to propose a model for tartrate utilization in A. vitis. ttuA encodes a LysR-like transcriptional activator and is transcribed in the absence of tartrate. ttuB codes for a protein with homology to transporter proteins and is required for entry of tartrate into bacteria. ttuC codes for a tartrate dehydrogenase, while ttuD lacks homology to known sequences; the growth properties of ttuD mutants suggest that TtuD catalyzes the second step in tartrate degradation. A fifth incomplete ORF (ttuE) encodes a pyruvate kinase which is induced by tartrate and required for optimal growth. Although the ttuABCD fragment allows growth of A. tumefaciens on tartrate, it does not provide full tartrate utilization in the original A. vitis background.In 1973, a new group of agrobacteria isolated from grapevines (Vitis vinifera) was described and classified as biotype III (17,32,46). Further studies demonstrated its close association with grapevine (4,5,23,33,34,42). This Agrobacterium group is now considered as a separate species: Agrobacterium vitis (30). It is the only well-studied Agrobacterium group with a narrow natural host range.Part of the host specificity of A. vitis has been attributed to a chromosomally encoded polygalacturonase responsible for grapevine root necrosis (3,27,38 (17,33,47).In planta mating experiments (31, 48) have shown that in several A. vitis strains the tartrate degradation genes are carried by conjugative plasmids. The tartrate utilization plasmid of the A. vitis nopaline strain AB4 (pTrAB4) was chosen for further studies because of its relatively small size (170 kb).In Pseudomonas spp., biochemical studies have revealed two tartrate degradation pathways, one involving a dehydratase (1,14,20,45) and the other a tartrate dehydrogenase (TDH) and a reductase (7,18,25) or an L(ϩ)-tartrate decarboxylase (12). An L(ϩ)/D(ϩ)-tartrate dehydratase is also found in Salmonella typhimurium (54). The tartrate dehydratase gene of Escherichia coli is homologous to class I fumarase genes (35). TDH is part of the second pathway and has been isolated from Pseudomonas putida (19,49,50) and Rhodopseudomonas sphaeroides (11). P. putida TDH catalyzes the degradation of L(ϩ)-tartrate, meso-tartrate, and D(ϩ)-malate at the same active site by using NADH 2 and Mn 2ϩ (50). The P. putida tdh gene has recently been sequenced (49). So far, n...

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“…Tartrate utilization plasmids (pTr) of A. vitis provide another example. They promote growth (TAR-I and TAR-II) on pTrAB3 and pTiAB3 respectively (Otten et al, 1995). The tar region consists of the ttuA-E genes, orfX, orfY and a second ttuC copy.…”

Section: Other Ecologically Significant Plasmidsmentioning

confidence: 99%

Agrobacterium: A disease-causing bacterium

Otten

Burr

Szegedi³

2008

Agrobacterium: From Biology to Biotechnology

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Agrobacterium vitisStrain AB3 Harbors Two Independent Tartrate Utilization Systems, One of Which Is Encoded by theTi Plasmid

Cited by 22 publications

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Construction of physical map and mapping of chromosomal virulence genes of the biovar 3 Agrobacterium (Rhizobium vitis) strain K-Ag-1

Construction of physical map and mapping of chromosomal virulence genes of the biovar 3 Agrobacterium (Rhizobium vitis) strain K-Ag-1

Sequence and mutational analysis of a tartrate utilization operon from Agrobacterium vitis

Agrobacterium: A disease-causing bacterium

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