<i>Agrobacterium rhizogenes</i>-Transformed Roots of <i>Medicago truncatula</i> for the Study of Nitrogen-Fixing and Endomycorrhizal Symbiotic Associations

Boisson-Dernier, Aurélien; Chabaud, Mireille; García, F. Sevillano; Bécard, Guillaume; Rosenberg, Charles; Barker, David G.

doi:10.1094/mpmi.2001.14.6.695

Cited by 647 publications

(585 citation statements)

References 31 publications

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“…The protocol was adapted from Boisson-Dernier et al (2001). Approximately 48 h after germination, the radicle was sectioned at 5 mm from the root tip with a sterile scalpel.…”

Section: Agrobacterium Rhizogenes-mediated Transgenic Root Transformamentioning

confidence: 99%

CLE Peptides Control Medicago truncatula Nodulation Locally and Systemically

Mortier

Herder²,

Whitford³

et al. 2010

Plant Physiology

294

403

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The CLAVATA3/embryo-surrounding region (CLE) peptides control the fine balance between proliferation and differentiation in plant development. We studied the role of CLE peptides during indeterminate nodule development and identified 25 MtCLE peptide genes in the Medicago truncatula genome, of which two genes, MtCLE12 and MtCLE13, had nodulation-related expression patterns that were linked to proliferation and differentiation. MtCLE13 expression was up-regulated early in nodule development. A high-to-low expression gradient radiated from the inner toward the outer cortical cell layers in a region defining the incipient nodule. At later stages, MtCLE12 and MtCLE13 were expressed in differentiating nodules and in the apical part of mature, elongated nodules. Functional analysis revealed a putative role for MtCLE12 and MtCLE13 in autoregulation of nodulation, a mechanism that controls the number of nodules and involves systemic signals mediated by a leucine-rich repeat receptor-like kinase, SUNN, which is active in the shoot. When MtCLE12 and MtCLE13 were ectopically expressed in transgenic roots, nodulation was abolished at the level of the nodulation factor signal transduction, and this inhibition involved long-distance signaling. In addition, composite plants with roots ectopically expressing MtCLE12 or MtCLE13 had elongated petioles. This systemic effect was not observed in transgenic roots ectopically expressing MtCLE12 and MtCLE13 in a sunn-1 mutant background, although nodulation was still strongly reduced. These results suggest multiple roles for CLE signaling in nodulation.

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“…The protocol was adapted from Boisson-Dernier et al (2001). Approximately 48 h after germination, the radicle was sectioned at 5 mm from the root tip with a sterile scalpel.…”

Section: Agrobacterium Rhizogenes-mediated Transgenic Root Transformamentioning

confidence: 99%

CLE Peptides Control Medicago truncatula Nodulation Locally and Systemically

Mortier

Herder²,

Whitford³

et al. 2010

Plant Physiology

294

403

View full text Add to dashboard Cite

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“…We explored this idea using an ProEnod11:b-glucuronidase (GUS) reporter construct (Boisson-Dernier et al, 2001) that is expressed in the nodule apical region as well as in the root tip in wild-type M. truncatula (Figures 1L and 1M;Journet et al, 2001;Svistoonoff et al, 2010). In noot plants, the expression of this fusion in the root tip is conserved ( Figure 1M).…”

Section: The Roots On the Mutant Organ May Originate From The Vasculamentioning

confidence: 99%

“…Four independent lines were used later for imaging and sectioning. Hairy root transformations were done as described by Boisson-Dernier et al (2001) using Agrobacterium rhizogenes strains Arqua1 containing the pEnod11:GUS plasmid. The transformed plantlets were transferred into sand/perlite medium for nodulation, as described above.…”

Section: Plant Transformationmentioning

confidence: 99%

NODULE ROOT and COCHLEATA Maintain Nodule Development and Are Legume Orthologs of Arabidopsis BLADE-ON-PETIOLE Genes

et al. 2012

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During their symbiotic interaction with rhizobia, legume plants develop symbiosis-specific organs on their roots, called nodules, that house nitrogen-fixing bacteria. The molecular mechanisms governing the identity and maintenance of these organs are unknown. Using Medicago truncatula nodule root (noot) mutants and pea (Pisum sativum) cochleata (coch) mutants, which are characterized by the abnormal development of roots from the nodule, we identified the NOOT and COCH genes as being necessary for the robust maintenance of nodule identity throughout the nodule developmental program. NOOT and COCH are Arabidopsis thaliana BLADE-ON-PETIOLE orthologs, and we have shown that their functions in leaf and flower development are conserved in M. truncatula and pea. The identification of these two genes defines a clade in the BTB/POZ-ankyrin domain proteins that shares conserved functions in eudicot organ development and suggests that NOOT and COCH were recruited to repress root identity in the legume symbiotic organ.

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“…These protein fusions (Fig. 6A) were expressed from the CaMV 35S promoter in roots of M. truncatula after Agrobacterium rhizogenes transformation (Boisson-Dernier et al, 2001). Many fluorescent lines of the EX and TM constructs were obtained.…”

Section: Localization and Targeting Of The Lecrks To The Pmmentioning

confidence: 99%

Characterization of Four Lectin-Like Receptor Kinases Expressed in Roots of Medicago truncatula. Structure, Location, Regulation of Expression, and Potential Role in the Symbiosis with Sinorhizobium meliloti

et al. 2003

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To study the role of LecRK (lectin-like receptor kinase) genes in the legume-rhizobia symbiosis, we have characterized the four Medicago truncatula Gaernt. LecRK genes that are most highly expressed in roots. Three of these genes, MtLecRK7;1, MtLecRK7;2, and MtLecRK7;3, encode proteins most closely related to the Class A LecRKs of Arabidopsis, whereas the protein encoded by the fourth gene, MtLecRK1;1, is most similar to a Class B Arabidopsis LecRK. All four genes show a strongly enhanced root expression, and detailed studies on MtLecRK1;1 and MtLecRK7;2 revealed that the levels of their mRNAs are increased by nitrogen starvation and transiently repressed after either rhizobial inoculation or addition of lipochitooligosaccharidic Nod factors. Studies of the MtLecRK1;1 and MtLecRK7;2 proteins, using green fluorescent protein fusions in transgenic M. truncatula roots, revealed that they are located in the plasma membrane and that their central transmembrane-spanning helix is required for correct sorting. Moreover, their lectin-like domains appear to be highly glycosylated. Of the four proteins, only MtLecRK1;1 shows a high conservation of key residues implicated in monosaccharide binding, and molecular modeling revealed that this protein may be capable of interacting with Nod factors. However, no increase in Nod factor binding was found in roots overexpressing a fusion in which the kinase domain of this protein had been replaced with green fluorescent protein. Roots expressing this fusion protein however showed an increase in nodule number, suggesting that expression of MtLecRK1;1 influences nodulation. The potential role of LecRKs in the legumerhizobia symbiosis is discussed.The lectin-like receptor kinases (LecRKs) are a class of proteins originally described from Arabidopsis (Hervé et al., 1996). They have a structure similar to other plant receptor-like kinases (RLKs; Shiu and Bleecker, 2001;Cock et al., 2002) with an N-terminal targeting signal, a presumably extracellular domain, a single transmembrane (TM)-spanning helix, and a cytosolic kinase domain. The Arabidopsis genome contains over 610 RLKs that have been shown to be monophylogenetic with respect to the kinase domain and most closely related to the fruitfly (Drosophila melanogaster) Pelle and related animal cytoplasmic kinases (Shiu and Bleecker, 2001). In cases in which they have been tested, these kinases have been shown to have specificity for phosphorylation on Ser/Thr residues (Shiu and Bleecker, 2001).The plant RLKs can be grouped into more than 21 structural classes based on their extracellular domains (Shiu and Bleecker, 2001). The LecRKs represent one such class in which the extracellular domain is related to legume lectins. The Arabidopsis genome contains at least 42 LecRK sequences that have been grouped into three classes (A-C) plus an additional nine sequences of related soluble lectins (Barre et al., 2002). Searches in plant databases reveal that LecRK genes are widespread in higher plants, but apart from Arabidopsis (Hervé et al., 1996,...

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Agrobacterium rhizogenes-Transformed Roots of Medicago truncatula for the Study of Nitrogen-Fixing and Endomycorrhizal Symbiotic Associations

Cited by 647 publications

References 31 publications

CLE Peptides Control Medicago truncatula Nodulation Locally and Systemically

CLE Peptides Control Medicago truncatula Nodulation Locally and Systemically

NODULE ROOT and COCHLEATA Maintain Nodule Development and Are Legume Orthologs of Arabidopsis BLADE-ON-PETIOLE Genes

Characterization of Four Lectin-Like Receptor Kinases Expressed in Roots of Medicago truncatula. Structure, Location, Regulation of Expression, and Potential Role in the Symbiosis with Sinorhizobium meliloti

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