<i>Agrobacterium</i>-mediated transformation of tomato (<i>Lycopersicon esculentum</i>L. cv. Hezuo 908) with improved efficiency

Sun, Sheng; Kang, Xin; Xing, Xiangzhuo; Xu, Xiaoyong; Cheng, Jiao; Zheng, Shu; Xing, Guoming

doi:10.1080/13102818.2015.1056753

Cited by 31 publications

(26 citation statements)

References 29 publications

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“…Five tissue culture jars containing seeds subjected to different sterilization treatment in three replicates were used. The cultures were kept initially for 48 h in the dark, and subsequently maintained at 23 ° ±2°C, with 30–50% humidity [42]. After the first 48 h, the cultures were switched to a 16/8 h light/dark photoperiod provided by 70 μmolm -2 s -1 cool white fluorescent lights in a growth room.…”

Section: Methodsmentioning

confidence: 99%

Efficient and reproducible somatic embryogenesis and micropropagation in tomato via novel structures - Rhizoid Tubers

et al. 2019

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A dual in vitro regeneration system consisting of indirect organogenesis and somatic embryogenesis (SE), applicable to several varieties of tomato— Solanum lycopersicum (cv. Riogrande , cv. Roma , hybrid 17905 and model cv. M82 ) has been established. This system is both improved and highly reproducible compared to current methods. Callus initiation, plant regeneration and SE was developed for one-week-old cotyledon explants. Indirect organogenesis via callus induction (CI) was developed for all four varieties of tomato used in this study. One-week-old tomato seedlings were used as a source of cotyledon and hypocotyl segments as explants. The explants were subsequently cultured on Murashige and Skoog (MS) medium supplemented with different combination and concentrations of plant growth regulators (PGRs). Substantial trends in regeneration and propagation response were observed among the varieties and treatments. For commercial varieties cvs. Riogrande and Roma , maximum CI was observed at 2 weeks in CIMT 9 (0.5 mg/L NAA, 1 mg/L BAP) and CIMT 12 (2 mg/L IAA, 2 mg/L NAA, 2 mg/L BAP, 4 mg/L KIN). However, cv. M82 responded after 4 weeks to a combination of treatments CIMT 9 (0.5 mg/L NAA + 1 mg/L BAP) and CIMT 13 (2 mg/L IAA + 2 mg/L NAA + 2 mg/L BAP + 4 mg/L ZEA) for the production of calli. Subsequent shoot and root organogenesis were optimized for all four varieties. Cv. Riogrande , exhibited fastidious in vitro regeneration potential and selected for induction of somatic embryos via SE involving novel structure: rhizoid tubers (RTBs). Numerous fine hair like rhizoids (~23/explants) were first developed from cotyledon and hypocotyl explants cultured on MS medium supplemented with 0.5 or 2 mg/L NAA at pH 4.0 in dark conditions. Further incubation of each rhizoid under light conditions on MS media supplemented with 5 mg/L TDZ or BAP at pH 4.0 led to the formation of a novel structure—rhizoid Tubers (RTBs). Thus, as evident from histology, SE in Riogrande tomato species requires a medium with pH of (4.0) and higher concentration of cytokinins (BAP/TDZ) to form on average 40–45 RTBs from both explants. Histological and morphological studies revealed that RTBs develop through different stages of embryogenesis to multiple plantlets, on MS medium with 5 mg/L TDZ/BAP at normal pH (5.8). The results obtained indicated that the induced somatic embryos of tomato with lower pH are a more efficient mode of propagation than the organogenesis with or without callus formation. The RTBs led to a complete plantlets regeneration in 45 days compared to indirect organogenesis at 60 days.

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Section: Methodsmentioning

confidence: 99%

Efficient and reproducible somatic embryogenesis and micropropagation in tomato via novel structures - Rhizoid Tubers

et al. 2019

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“…The pBI121-35S- SlDREBA4 fusion plasmid was introduced into Agrobacterium tumefaciens strain GV3101. Positive GV3101 was transformed into Microtom cotyledons ( Sun et al., 2015 ), and the rooting plants were screened by kanamycin and transferred to Hoagland’s nutrient solution. The level of SlDREBA4 transcription was assessed using RT-qPCR with the primer, pSlDREBA4-q.…”

Section: Methodsmentioning

confidence: 99%

Characterization of the DREBA4-Type Transcription Factor (SlDREBA4), Which Contributes to Heat Tolerance in Tomatoes

et al. 2020

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Dehydration-responsive element binding (DREB) transcription factors play crucial regulatory roles in abiotic stress. The only DREB transcription factor in tomato (Solanum lycopersicum), SlDREBA4 (Accession No. MN197531), which was determined to be a DREBA4 subfamily member, was isolated from cv. Microtom using high-temperature-induced digital gene expression (DGE) profiling technology. The constitutive expression of SlDREBA4 was detected in different tissues of Microtom plants. In addition to responding to high temperature, SlDREBA4 was up-regulated after exposure to abscisic acid (ABA), cold, drought and high-salt conditions. Transgenic overexpression and silencing systems revealed that SlDREBA4 could alter the resistance of transgenic Microtom plants to heat stress by altering the content of osmolytes and stress hormones, and the activities of antioxidant enzymes at the physiologic level. Moreover, SlDREBA4 regulated the downstream gene expression of many heat shock proteins (Hsp), as well as calcium-binding protein enriched in the pathways of protein processing in endoplasmic reticulum (ko04141) and plant-pathogen interaction (ko04626) at the molecular level. SlDREBA4 also induces the expression of biosynthesis genes in jasmonic acid (JA), salicylic acid (SA), and ethylene (ETH), and specifically binds to the DRE elements (core sequence, A/GCCGAC) of the Hsp genes downstream from SlDREBA4. This study provides new genetic resources and rationales for tomato heat-tolerance breeding and the heat-related regulatory mechanisms of DREBs.

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“…A higher efficacy of transformation was reported in the case of flowers treated with a bacterial suspension before pollination. Despite promising transformation efficiency, some adverse changes in the morphology (short and not erected steam, curled leaves) of the plants were observed in comparison Sun et al (2015) to wild-type (WT) plants. Although the flowers on these plants appeared earlier and were normal, unfortunately they were sterile and did not give fruits (Yasmeen et al 2009).…”

Section: Methodology Of Tomato Transformationmentioning

confidence: 99%

“…The agroinfection process is complex, and its efficiency depends on a broad spectrum of elements including the presence of a chemoattractant in the culture or preculture media, the application of nurse cells, bacterial factors (culture density, virulence of the Agrobacterium strain), the type of plasmid vector and the tissue specific factors (the type of explants and the genotype), the composition of the culture media (concentration of phytohormones), the concentration and kind of selective agents and the cocultivation time Chetty et al 2013;Shah et al 2015;Sun et al 2015). Examples of optimisation of aforementioned parameters for tomato transformation are presented in Table 1.…”

Section: Methodology Of Tomato Transformationmentioning

confidence: 99%

Tomato tolerance to abiotic stress: a review of most often engineered target sequences

Gerszberg

Hnatuszko-Konka

2017

Plant Growth Regul

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Agrobacterium-mediated transformation of tomato (Lycopersicon esculentumL. cv. Hezuo 908) with improved efficiency

Cited by 31 publications

References 29 publications

Efficient and reproducible somatic embryogenesis and micropropagation in tomato via novel structures - Rhizoid Tubers

Efficient and reproducible somatic embryogenesis and micropropagation in tomato via novel structures - Rhizoid Tubers

Characterization of the DREBA4-Type Transcription Factor (SlDREBA4), Which Contributes to Heat Tolerance in Tomatoes

Tomato tolerance to abiotic stress: a review of most often engineered target sequences

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