<i>Agrobacterium</i> in the Genomics Age

Gelvin, Stanton B.

doi:10.1104/pp.109.139873

Cited by 105 publications

(48 citation statements)

References 158 publications

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“…Many of the examples of HGT between prokaryotes and unicellular (Andersson, 2005;Loftus et al, 2005;Nosenko and Bhattacharya, 2007) or multicellular eukaryotes (Nikoh et al, 2008;Starcevic et al, 2008) are associated with parasitism or phagotrophy (reviewed by Bock, 2010). The classic example of HGT from a prokaryote to a multicellular eukaryote is the transfer of DNA via the Agrobacterium Ti plasmid to plants (reviewed by Gelvin, 2009). However, more recently, HGT has been elegantly demonstrated between the Wolbachia bacterium and a number of invertebrates (beetles, fruit flies, mosquitoes and nematodes), and biological functionality has been shown as well in some cases (Kondo et al, 2002;Nikoh et al, 2008;Wasmuth et al, 2008;Klasson et al, 2009;Woolfit et al, 2009).…”

Section: Horizontal Gene Transfermentioning

confidence: 99%

The making of a photosynthetic animal

Rumpho

Pelletreau

Moustafa

et al. 2011

Journal of Experimental Biology

145

124

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SummarySymbiotic animals containing green photobionts challenge the common perception that only plants are capable of capturing the sun's rays and converting them into biological energy through photoautotrophic CO 2 fixation (photosynthesis). 'Solar-powered' sacoglossan molluscs, or sea slugs, have taken this type of symbiotic association one step further by solely harboring the photosynthetic organelle, the plastid (chloroplast). One such sea slug, Elysia chlorotica, lives as a 'plant' when provided with only light and air as a result of acquiring plastids during feeding on its algal prey Vaucheria litorea. The captured plastids (kleptoplasts) are retained intracellularly in cells lining the digestive diverticula of the sea slug, a phenomenon sometimes referred to as kleptoplasty. Photosynthesis by the plastids provides E. chlorotica with energy and fixed carbon for its entire lifespan of 10months. The plastids are not transmitted vertically (i.e. are absent in eggs) and do not undergo division in the sea slug. However, de novo protein synthesis continues, including plastid-and nuclear-encoded plastid-targeted proteins, despite the apparent absence of algal nuclei. Here we discuss current data and provide hypotheses to explain how long-term photosynthetic activity is maintained by the kleptoplasts. This fascinating 'green animal' provides a unique model to study the evolution of photosynthesis in a multicellular heterotrophic organism. Supplementary material available online at

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Section: Horizontal Gene Transfermentioning

confidence: 99%

The making of a photosynthetic animal

Rumpho

Pelletreau

Moustafa

et al. 2011

Journal of Experimental Biology

145

124

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“…These results confirmed the transgenic nature of the three selected hairy root lines. This transformation was a consequence of an integration of the bacterial rolB gene into the plant genome (White et al 1985;Gelvin 2009). …”

Section: Confirmation Of Transgenic Hairy Root Linesmentioning

confidence: 99%

Screening and kinetic studies of catharanthine and ajmalicine accumulation and their correlation with growth biomass inCatharanthus roseushairy roots

Benyammi¹,

Paris

Khelifi-Slaoui³

et al. 2016

Pharmaceutical Biology

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Context Catharanthus roseus (L.) G. Don (Apocynaceae) is still one of the most important sources of terpene indole alkaloids including anticancer and hypertensive drugs as vincristine and vinblastine. These final compounds have complex pathway and many enzymes are involved in their biosynthesis. Indeed, ajmalicine and catharanthine are important precursors their increase can lead to enhance levels of molecules of interest. Objective This study aims at selecting the highest yield of hairy root line(s) and at identifying best times for further treatments. We study kinetics growth and alkaloids (ajmalicine and catharanthine) accumulation of three selected hairy root lines during the culture cycle in order to determine the relationship between biomass production and alkaloids accumulation. Materials and methods Comparative analysis has been carried out on three selected lines of Catharanthus roseus hairy roots (LP10, LP21 and L54) for their kinetics of growth and the accumulation of ajamalicine and catharanthine, throughout a 35-day culture cycle. The methanolic extract for each line in different times during culture cycle is analyzed using liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS). Results Maximum accumulation of the alkaloids is recorded for LP10 line in which the peak of ajmalicine and catharanthine accumulation reached to 3.8 and 4.3 mg/g dry weight (DW), respectively. This increase coincides with an exponential growth phase. Discussion and conclusion Our results suggest that the evolution of accumulation of ajmalicine and catharanthine are positively correlated with the development of the biomass growth. Significantly, for LP10 line the most promising line to continue optimizing the production of TIAs. Additionally, the end of exponential phase remains the best period for elicitor stimuli. ARTICLE HISTORY

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“…2). Probable stationary phase of Agrobacterium and toxicity due to phenolic exudates from wounded PLBs at extended incubation period [22] caused the downfall in terms of chemotactic response.…”

Section: Chemotaxis Assaymentioning

confidence: 99%

Mapping of the Interaction Between Agrobacterium tumefaciens and Vanda Kasem’s Delight Orchid Protocorm-Like Bodies

Gnasekaran

Subramaniam

2015

Indian J Microbiol

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Physical contact between A. tumefaciens and the target plant cell walls is essential to transfer and integrate the transgene to introduce a novel trait. Chemotaxis response and attachment of Agrobacterium towards Vanda Kasem's Delight (VKD) protocorm-like bodies (PLBs) were studied to analyse the interaction between Agrobacterium and PLB during the transformation event. The study shows that initially A. tumefaciens reversibly attached to PLB surface via polar and lateral mode of adherence followed by the irreversible attachment which involved the production of cellulosic fibril by A. tumefaciens. Cellulosic fibril allows formation of biofilm at the tip of trichome. Contrarily, attachment mutant Escherichia coli strain DH5a was significantly deficient in the attachment process. Spectrophotometric GUS assay showed the mean value of attachment by A. tumefaciens was 8.72 % compared to the negative control E. coli strain DH5a that produced 0.16 %. A. tumefaciens swarmed with sharper and brighter edge when severe wounding was applied to the PLBs producing the highest swarming ratio of 1.46 demonstrating the positive effect of the plant exudates on bacterial movement. The study shows that VKD's PLBs are the suitable explants for Agrobacterium-mediated transformation since the bacteria expressed higher competency rate.

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Agrobacterium in the Genomics Age

Cited by 105 publications

References 158 publications

The making of a photosynthetic animal

The making of a photosynthetic animal

Screening and kinetic studies of catharanthine and ajmalicine accumulation and their correlation with growth biomass inCatharanthus roseushairy roots

Mapping of the Interaction Between Agrobacterium tumefaciens and Vanda Kasem’s Delight Orchid Protocorm-Like Bodies

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