<i>AGL80</i>Is Required for Central Cell and Endosperm Development in<i>Arabidopsis</i>

Portereiko, Michael F.; Lloyd, A. C.; Steffen, Joshua G.; Punwani, Jayson A.; Otsuga, Denichiro; Drews, Gary N.

doi:10.1105/tpc.106.040824

Cited by 212 publications

(212 citation statements)

References 58 publications

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“…Also, the function of the different Type I genes is generally poorly characterized. However, several Type I genes have been reported to play a role in female gametogenesis, embryogenesis, and seed development (Portereiko et al, 2006;Bemer et al, 2010;Masiero et al, 2011).…”

Section: Iii2 Type I Mads-box Genesmentioning

confidence: 99%

Phylogenomic Synteny Network Analysis of MADS-Box Transcription Factor Genes Reveals Lineage-Specific Transpositions, Ancient Tandem Duplications, and Deep Positional Conservation

et al. 2017

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Conserved genomic context provides critical information for comparative evolutionary analysis. With the increase in numbers of sequenced plant genomes, synteny analysis can provide new insights into gene family evolution. Here, we exploit a network analysis approach to organize and interpret massive pairwise syntenic relationships. Specifically, we analyzed synteny networks of the MADS-box transcription factor gene family using 51 completed plant genomes. In combination with phylogenetic profiling, several novel evolutionary patterns were inferred and visualized from synteny network clusters. We found lineage-specific clusters that derive from transposition events for the regulators of floral development (APETALA3 and PI) and flowering time (FLC) in the Brassicales and for the regulators of root development (AGL17) in Poales. We also identified two large gene clusters that jointly encompass many key phenotypic regulatory Type II MADS-box gene clades (SEP1, SQUA, TM8, SEP3, FLC, AGL6, and TM3). Gene clustering and gene trees support the idea that these genes are derived from an ancient tandem gene duplication that likely predates the radiation of the seed plants and then expanded by subsequent polyploidy events. We also identified angiosperm-wide conservation of synteny of several other less studied clades. Combined, these findings provide new hypotheses for the genomic origins, biological conservation, and divergence of MADS-box gene family members.

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Section: Iii2 Type I Mads-box Genesmentioning

confidence: 99%

Phylogenomic Synteny Network Analysis of MADS-Box Transcription Factor Genes Reveals Lineage-Specific Transpositions, Ancient Tandem Duplications, and Deep Positional Conservation

et al. 2017

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“…The DD46 promoter (At1g22015) has been shown to be active in the central cell and the synergid cells of the female gametophyte (Portereiko et al, 2006). We established transgenic plants containing the DD46 promoter fused to the ␤-GLUCURONIDASE (GUS) reporter gene (referred to as DD46::GUS) and investigated the temporal and spatial expression pattern of this reporter construct.…”

Section: Expression Of Msi1 In the Female Gametophyte Can Rescue Thementioning

confidence: 99%

Polycomb group proteins function in the female gametophyte to determine seed development in plants

et al. 2007

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Polycomb group (PcG) proteins are evolutionary conserved proteins that stably maintain established transcriptional patterns over cell generations. The FERTILIZATION INDEPENDENT SEED (FIS) PcG complex from plants has a similar composition to the Polycomb repressive complex 2 from animals. Mutations in FIS genes cause parent-of-origin-dependent seed abortion. Every seed inheriting a mutant fis allele from the mother is destined to abort, regardless of the presence of a wild-type paternal allele. We tested in Arabidopsis whether the parent-of-origin-dependent seed abortion caused by lack of the FIS subunit MSI1 is caused by parental imprinting of the MSI1 gene. Our data show that MSI1 is not an imprinted gene and that early paternal MSI1 expression is not sufficient to rescue msi1 mutant seeds. By contrast, expression of MSI1 in msi1 female gametophytes is necessary to restore normal seed development, strongly arguing that the female gametophytic effect of fis mutants is caused by a functional requirement for an intact FIS complex in the female gametophyte. Thus, FIS-mediated expression patterns established in the female gametophyte can impact on seed development, establishing fis mutants as true female gametophytic maternal-effect mutants.

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“…The qPCR cycling was performed at 95 1C for 5 min followed by 40 cycles of denaturation (95 1C for 15 s), annealing (61 1C for 30 s) and extension (72 1C for 20 s). The target mRNA level was calculated as described previously (Portereiko et al, 2006).…”

Section: Quantitative Real-time Pcr (Qpcr)mentioning

confidence: 99%

Downregulation of tumor suppressor Pdcd4 promotes invasion and activates both β-catenin/Tcf and AP-1-dependent transcription in colon carcinoma cells

2007

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Programmed cell death 4 (Pdcd4) is a tumor suppressor that inhibits neoplastic transformation and tumor invasion. Tissue microarray analysis showed that Pdcd4 expression is downregulated in colon adenocarcinoma and carcinoma relative to adjacent normal tissues. To address the issue of whether reduced Pdcd4 expression is sufficient to promote tumor progression, we knocked down Pdcd4 expression in colon tumor HT29 cells using pdcd4 short hairpin RNA (shRNA). Pdcd4 knockdown results in a fibroblast-like transition, while the control cells (expressing LacZ shRNA) remain as clumped similar to the parental cells. In addition, expression of pdcd4 shRNA in HT29 cells promotes invasion. In an effort to characterize the molecular mechanism underlying these observations, we discovered that knockdown of Pdcd4 results in reduction of E-cadherin expression, and accumulation of active b-catenin in the nucleus. The active b-catenin binds with T-cell factor 4 (Tcf4) and activates b-catenin/ Tcf-dependent transcription. Furthermore, Pdcd4 knockdown dramatically increases AP-1-dependent transcription. Thus, the mechanism by which reduced Pdcd4 expression promotes invasion appears to involve the activation of b-catenin/Tcf and AP-1-dependent transcription.

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AGL80Is Required for Central Cell and Endosperm Development inArabidopsis

Cited by 212 publications

References 58 publications

Phylogenomic Synteny Network Analysis of MADS-Box Transcription Factor Genes Reveals Lineage-Specific Transpositions, Ancient Tandem Duplications, and Deep Positional Conservation

Phylogenomic Synteny Network Analysis of MADS-Box Transcription Factor Genes Reveals Lineage-Specific Transpositions, Ancient Tandem Duplications, and Deep Positional Conservation

Polycomb group proteins function in the female gametophyte to determine seed development in plants

Downregulation of tumor suppressor Pdcd4 promotes invasion and activates both β-catenin/Tcf and AP-1-dependent transcription in colon carcinoma cells

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