Agaricus subrufescens and Pleurotus ostreatus mushrooms as alternative additives to antibiotics in diets for broilers challenged with Eimeria spp.

Lima, Gabrieli Andressa de; Barbosa, Bruna-Viana; Araujo, Robert Guaracy Aparecido Cardoso; Polidoro, B. R.; Polycarpo, Gustavo do Valle; Zied, Diego Cunha; Biller, Jaqueline Dalbello; Ventura, Gabriela; Modesto, I. M.; Madeira, A. M. B. N.; Cruz-Polycarpo, Valquíria Cação

doi:10.1080/00071668.2020.1837344

Cited by 11 publications

(13 citation statements)

References 51 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Looking specifically at some 2021 publications, if pre-screening with in vitro models had been used by Fu et al (2021), concentrations could have been adjusted to reduce animals from 630 to 480 (23.8%). Similarly, the use of in vitro models could have excluded one testing group for both Lima et al (2021) and Herrero-Encinas et al (2021), reducing animals from 900 to 720 (20%) and 400 to 320 (20%), respectively. If we extrapolate these data (an average of 20% reduction) to the total published studies in 2021, the use of our in vitro models could have avoided the experimental use of 792 chickens (out of 3,958) globally, or 3,958 (out of 19,786) in the last 5 years.…”

Section: Discussionmentioning

confidence: 99%

Reduction of chickens use to perform in vitro pre-screening of novel anticoccidials by miniaturisation and increased throughput of the current Eimeria tenella compound-screening model

et al. 2022

View full text Add to dashboard Cite

We have developed an in vitro model for the evaluation of potential anticoccidial properties of novel compounds aimed to control chicken coccidiosis, a costly disease for the poultry industry. This disease is caused by protozoan parasites of the genus Eimeria (Apicomplexa), and it is mainly controlled by chemoprophylaxis with ionophores and chemical anticoccidials; however, there is an overall agreement about the limitation of these traditional drugs and the need to improve current methods of control. Anticoccidial activities of novel compounds is currently evaluated by expensive experiments that involve large numbers of chickens. The use of our in vitro model for the pre-screening of essential oils led to a reduction of 67% of the chickens used in the in vivo trials for validation. Eimeria parasites can only complete their life cycle in their animal host, therefore chickens are required for their propagation as they cannot be propagated in vitro. In this study, we describe how further optimisation of this in vitro model by miniaturisation can have an additional impact in reduction of the number of chickens used for the generation of parasite stocks for provision of the in vitro model. We have estimated that the use of one chicken could support the evaluation of 10 compounds with a 96-well plate format versus only two compounds with a 24-well plate format, which means an 80% reduction in chicken use. In this study we have proved that the miniaturisation into a 96-well plate format perfectly mimics the invasion and replication observed before in the 24-well plate format. In addition, the 96-well plate format has allowed the simultaneous pre-screening of higher numbers of anticoccidial drugs at different concentrations following streamlined protocols in a more cost-effective way, factors that are beneficial for a wider uptake of the model by other researchers investigating anticoccidial compounds.

show abstract

Section: Discussionmentioning

confidence: 99%

Reduction of chickens use to perform in vitro pre-screening of novel anticoccidials by miniaturisation and increased throughput of the current Eimeria tenella compound-screening model

et al. 2022

View full text Add to dashboard Cite

show abstract

“…In recent years, a variety of Chinese herbal extracts, such as Shi Ying Zi [ 27 ], berberine [ 28 ], the mushrooms Agaricus subrufescens and Pleurotus ostreatus [ 29 ], Cinnamomum verum [ 30 ] and Canary rue [ 31 ], have been reported to show anticoccidial effects to some extent. EA, a polyphenol extracted from certain medicinal plants, is a member of the tannin family that possesses several biological properties, including antioxidant and anti-inflammatory properties [ 32 , 33 ].…”

Section: Discussionmentioning

confidence: 99%

Oral administration of Lactobacillus brevis 23017 combined with ellagic acid attenuates intestinal inflammatory injury caused by Eimeria infection by activating the Nrf2/HO-1 antioxidant pathway

Yang

Pan

Jia

et al. 2022

Vet Res

View full text Add to dashboard Cite

The aim of this study was to investigate whether oral administration of Lactobacillus brevis 23017 (LB) alone and in combination with ellagic acid inhibits ChTLR15/ChNLRP3/ChIL-1β by activating the Nrf2/HO-1 pathway to attenuate intestinal inflammatory injury. Two animal experiments were performed. In Experiment 1, chickens were allocated into 7 groups: PBS, and low, medium and high dosages of live and heat-killed LB, named L/LB(+), M/LB(+) and H/LB(+), and L/LB(−), M/LB(−) and H/LB(−), respectively. In Experiment 2, chickens were divided into 5 groups: PBS, challenge control, and low, medium and high dosages of ellagic acid combined with LB(+), named L/EA + L/LB(+), M/EA + M/LB(+) and H/EA + H/LB(+), respectively. Chickens were gavaged with LB with or without ellagic acid once a day. Then, the mRNA and protein levels of the components of the Nrf2/HO-1 pathway found in the caecal tissues were quantified. On Day 7 post-infection with E. tenella, the levels of the components of the ChTLR15/NLRP3/IL-1β pathway in the caeca were again quantified, and the anticoccidial effects were assessed. The results showed that the levels of the genes in the Nrf2/HO-1 pathway in the chickens in the LB(+) groups were higher than those in the LB(−) groups (p < 0.001); those in the H/LB(+) group were higher than those in the M/LB(+) and L/LB(+) groups (p < 0.001); and those in the H/EA + H/LB(+) group showed the highest expression levels compared with the other groups (p < 0.001). After challenge, the chickens in the H/LB(+) group displayed less inflammatory injury than those in the M/LB(+) and L/LB(+) groups (p < 0.05), and the chickens in the H/EA + H/LB(+) group showed stronger anti-inflammatory effects than the other groups (p < 0.05). Thus, these protective effects against infection were consistent with the above results. Overall, significant anti-inflammatory effects were observed in chickens orally gavaged with high dosages of live L. brevis 23017 and ellagic acid, which occurred by regulation of the ChTLR15/NLRP3/IL-1β pathway.

show abstract

“…Therefore the investigation's results show better performance in both experimental and statistical analysis, but it has some limitations (Lima et al 2021). They do not perform so well on large data sets.…”

Section: Discussionmentioning

confidence: 99%

“…They generally fill in timberlands in relationships on woody pieces of trees either as nutrients, saprophyte or as symbionts in the dirt (Nair, Varsha, and Sunil 2021). Mushrooms and parasites overall are non-green creatures lacking chlorophyll (Lima et al 2021). They can't make their own food from basic inorganic materials, like water, carbon dioxide, and nitrates, utilizing energy from the sun.…”

Section: Introductionmentioning

confidence: 99%

Prediction of Insufficient Accuracy for Mushroom Classification whether Poisonous or Eatable Mushrooms using Support Vector Machine by comparing Logistic Regression to Improve Accuracy

2022

pnr

View full text Add to dashboard Cite

Aim: The main objective of the research study is to improve the good quality production for the better accuracy of eatable or poisonous mushrooms. It ought to be checked for consumable mushrooms. Exact assurance and appropriate distinguishing proof of species are the main safe approach to guarantee that the eatable mushrooms are poisonous, and defend against potential mishaps of burning through harmful one. Materials and Methods: The study used 51 samples with two groups of algorithms and the G-power worth of 80%. The mushrooms were collected from various web sources with recent study findings and threshold 0.05%, confidence interval 95% mean and standard deviation. To predict the Mushroom accuracy rate for already the Logistic Regression (LR) algorithm has found 90.46% of accuracy, therefore this study needs to find better accuracy for accuracy decrease prediction with the Novel Support Vector Machine (SVM) in Machine Learning.Results: This research study found 91.37% of accuracy for poisonous detection on food using the Support Vector Machine algorithm with a statistically significant difference between the two groups (p=0.022; p<0.05) with 95% confidence interval. Conclusion: This study concludes that the Novel Support Vector Machine algorithm on accuracy is significantly better than the Logistic Regression algorithm.

show abstract

Agaricus subrufescens and Pleurotus ostreatus mushrooms as alternative additives to antibiotics in diets for broilers challenged with Eimeria spp.

Cited by 11 publications

References 51 publications

Reduction of chickens use to perform in vitro pre-screening of novel anticoccidials by miniaturisation and increased throughput of the current Eimeria tenella compound-screening model

Reduction of chickens use to perform in vitro pre-screening of novel anticoccidials by miniaturisation and increased throughput of the current Eimeria tenella compound-screening model

Oral administration of Lactobacillus brevis 23017 combined with ellagic acid attenuates intestinal inflammatory injury caused by Eimeria infection by activating the Nrf2/HO-1 antioxidant pathway

Prediction of Insufficient Accuracy for Mushroom Classification whether Poisonous or Eatable Mushrooms using Support Vector Machine by comparing Logistic Regression to Improve Accuracy

Contact Info

Product

Resources

About