Agaricus blazeiextract attenuates rotenone-induced apoptosis through its mitochondrial protective and antioxidant properties in SH-SY5Y neuroblastoma cells

Abstract

The present study was aimed to find out the effect of Agaricus blazei mushroom extract against rotenone-induced cellular model. SH-SY5Y neuroblastoma cells are divided into four experimental groups (control, rotenone (100 nM), A. blazei (5 μg/ml) + rotenone (100 nM), and A. blazei alone treated) based on MTT assay, cells were allowed to measure the ROS, TBARS levels, and antioxidants activities. Finally, mitochondrial transmembrane potential (MMP) and expressions of apoptotic proteins were also analyzed. Pre-treatment with A. blazei significantly enhanced cell viability, attenuated rotenone-induced ROS, MMP, and apoptosis. Our results indicated that anti-apoptotic properties of this natural compound due to its antioxidant and mitochondrial protective function protect rotenone-induced cytotoxicity. Therefore, it may be concluded that A. blazei can be further developed as a promising drug for the treatment of Parkinson's disease (PD).

Apoptosis induction by Pleurotus sajor-caju (Fr.) Singer extracts on colorectal cancer cell lines

“…So, it is important to explore how Pleurotus sajor caju (PSC) constituents may regulate the cell dynamics and reduce cell proliferation. Identification of isolated compounds or extracts that induce the apoptotic cascade to reduce proliferation rates of cancer cells would be an effective strategy to control cancer progression (Venkatesh Gobi et al, 2018). The aim of this study was to investigate the effect of PSC extracts, specifically the PSC n-hexane extract (PSC-hex) in the proliferation of colorectal cancer cells (HCT116 wt , -Bax , -p21 and -p53 ), and to correlate the observed anti-proliferation activity to activation of proapoptotic and/or cell arrest regulation pathways.…”

Momordica charantia Ethanol Extract Attenuates H2O2-Induced Cell Death by Its Antioxidant and Anti-Apoptotic Properties in Human Neuroblastoma SK-N-MC Cells

“…This implies that MCEE inhibits the intrinsic apoptosis pathway by alleviating mitochondrial dysfunction by oxidative stress. Several studies have supported the notion that various natural product extracts inhibit apoptosis induced by H 2 O 2 [ 32 ], rotenone [ 8 ], and glutamate [ 11 ] in human and/or rat neuronal cell lines and this has been confirmed by the increased depolarized ΔΨm, Bax/Bcl2 ratio, and cytochrome c expression and activated caspases. It has been suggested that the natural product extracts used in each study protect cells through inhibition of the mitochondrial-dependent apoptosis pathway.…”

“…When the level of depolarized ΔΨ m increases, the cytochrome c present in the mitochondrial intermembrane space is released into the cytoplasm, which activates caspase-9 by binding to apoptosis protease activating factor (APAF)-1. Activated caspase-9 activates caspase-3 and results in apoptotic cell death [ 8 , 13 , 34 , 48 ]. The mitochondria-dependent cell death cascade is regulated by the Bcl2 family.…”

“…These cognitive and motor impairments affect the life quality and life span of elderly individuals [ 7 ]. According to a World Health Organization (WHO) report (2005), the ND-related mortality rate will continue to increase to the second most common cause of death, following cardiovascular disease, by 2040 [ 8 ]. For this reason, identification of therapeutic agents for NDs is required for public health in modern society, especially considering the rapidly expanding elderly population [ 9 ].…”

“…Various natural products have long been used as traditional medicines for the treatment of NDs [ 16 ]. Many studies have suggested that neuronal cells are protected from oxidative stress-induced cell damage by polyphenolic compounds, which are obtained through the extraction of natural products [ 8 ]. Momordica charantia (MC), known as bitter melon or bitter gourd, is widely grown and usually consumed as an important medicinal plant in various regions of Asia, Africa, Central Asia, and South America [ 17 , 18 ].…”