<i>Aeromonas veronii</i> and aerolysin are important for the pathogenesis of motile aeromonad septicemia in cyprinid fish

Ran, Chao; Qin, Chubin; Xie, Mingxu; Zhang, Jinxiong; Li, Jie; Xie, Yadong; Wang, Yibing; Li, Shuning; Liu, Lihui; Fu, Xiaozhe; Lin, Qiang; Li, Ningqiu; Liles, Mark R.; Zhou, Zhigang

doi:10.1111/1462-2920.14390

Cited by 117 publications

(65 citation statements)

References 55 publications

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“…IL-1β and TNF-α in response to colonisation and increases neutrophil recruitment to the wound site (Saraceni, Romero, Figueras, & Novoa, 2016 composition, concomitantly increasing pathogen abundance and decreasing beneficial intestinal bacteria . Although both A. hydrophila and A. veronii are able to colonise the intestine and cause mortality (Ran et al, 2018;Saraceni et al, 2016), A. veronii expresses more aerolysin toxin, which causes intestinal lesions and invasion of the intestinal barrier and is associated with increased virulence compared to A. hydrophila (Ran et al, 2018).…”

Section: Members Of the Genus Aeromonas That Affect Humans Includementioning

confidence: 99%

The zebrafish as a model for gastrointestinal tract–microbe interactions

Flores

Nguyen

Odem

et al. 2020

Cellular Microbiology

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The zebrafish (Danio rerio) has become a widely used vertebrate model for bacterial, fungal, viral, and protozoan infections. Due to its genetic tractability, large clutch sizes, ease of manipulation, and optical transparency during early life stages, it is a particularly useful model to address questions about the cellular microbiology of host-microbe interactions. Although its use as a model for systemic infections, as well as infections localised to the hindbrain and swimbladder having been thoroughly reviewed, studies focusing on host-microbe interactions in the zebrafish gastrointestinal tract have been neglected. Here, we summarise recent findings regarding the developmental and immune biology of the gastrointestinal tract, drawing parallels to mammalian systems. We discuss the use of adult and larval zebrafish as models for gastrointestinal infections, and more generally, for studies of host-microbe interactions in the gut. K E Y W O R D S Danio rerio, gastrointestinal tract, host-pathogen interactions, infection model, microbiome, microbiota, zebrafish

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Section: Members Of the Genus Aeromonas That Affect Humans Includementioning

confidence: 99%

The zebrafish as a model for gastrointestinal tract–microbe interactions

Flores

Nguyen

Odem

et al. 2020

Cellular Microbiology

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“…The purified bacteria were inoculated in a microbiological reaction tube (Hangzhou Microbe Reagent Co., Ltd., Hangzhou, China) according to the manufacturer's specifications. We conducted a preliminary identification of strains, and the test results were referenced using the System Identification Manual of Common Bacteria and Bergey's Manual of Systematic Bacteriology [18,19].…”

Section: Separation Purification and Physiological And Biochemical mentioning

confidence: 99%

Aeromonas veronii Infection in Commercial Freshwater Fish: A Potential Threat to Public Health

Li¹,

Raza

Yang³

et al. 2020

Animals

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Aeromonas veronii is an important pathogen causing freshwater fish sepsis and ulcer syndrome. An increasing number of cases have demonstrated its significance as an aquatic zoonotic agent. The purpose of this study was to ensure the safety of freshwater products by evaluating the infection status of edible freshwater fish. In this experiment, we isolated A. veronii from several species of apparently healthy freshwater fish, including Carassius auratus, Cyprinus carpio, Ctenopharyngodon idella, and Silurus asotus. A. veronii was identified through bacterial staining, culture characteristics, and 16S rDNA gene sequence. In addition, polymerase chain reaction (PCR) was used to investigate the distribution of seven major virulence genes, including aerolysin (aer: 88.51%), cytotoxic enterotoxin (act: 71.26%), serine proteinase (ser: 54.02%), adhesin (Aha: 40.23%), phospholipase (lip: 45.98%), nuclease (exu: 51.72%), and quorum sensing-controlled virulence factor (LuxS: 59.77%). In total, 496 strains of Aeromonas were isolated, including 87 strains of A. veronii. The isolates of A. veronii were Gram-negative, rod-shaped bacteria, and the colonies are yellow on Rimler-Shotts (RS) medium and showed greater than 99% homology with A. veronii ATCC35624 according to analyses of the 16S rDNA sequence. Nearly 50% of the A. veronii isolates carried at least four or more virulence genes, 25% of the isolates carried at least five types of virulence genes, and 59.77% isolates carried the LuxS gene, and the isolates carrying more virulence genes were found to be more virulent. These results are of great significance for further improving the food safety assessment of freshwater aquatic products.

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“…are believed to reflect the microflora of the aquatic habitat but with roles as primary and opportunistic pathogens. 7,34–38 A statistically increased detection rate of Aeromonas spp. in the moribund/mortality group, including isolation from 38% of tested moribund fish, suggests its role as a pathogen.…”

Section: Discussionmentioning

confidence: 99%

Exploring the advantages and limitations of sampling methods commonly used in research facilities for zebrafish health inspections

et al. 2019

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Examining zebrafish populations for the presence of disease is an integral component of managing fish health in research facilities. Currently, many different strategies are used for zebrafish fish health inspections, which is a scenario that may result in subjective and biased diagnostic evaluations. The goal of this study was to compare the success of pathogen detection between a sample size of randomly selected fish ( n = 60) that provides 95% confidence in pathogen detection based on a presumed pathogen prevalence level ≥5%, and other subpopulations and sample numbers commonly submitted for diagnostic testing within a 1000 tank, 30,000 fish, recirculating research system. This included fish collected from a sump tank ( n = 53), sentinel fish ( n = 11), and fish that were found moribund or freshly dead ( n = 18). Additionally, five fish from each subpopulation were collected for histopathologic examination. A second study used retrospective data to examine pathogen distribution between systems ( n = 2−5) in multi-system facilities ( n = 5) using a sample size of 60 fish per system. For the pathogens detected, results supported the use of representative sample numbers rather than smaller numbers of populations considered more at risk. The exception to this is for the moribund/mortality group, which may be a resource for targeted surveillance of select pathogens. Each system within multi-system facilities should be considered separate units in terms of fish health inspections and biosecurity. Development of these evidence-based standards for fish health inspections in zebrafish systems enhances fish welfare, provides identification of potentially zoonotic pathogens, and ensures scientific integrity and reproducibility of research results.

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Aeromonas veronii and aerolysin are important for the pathogenesis of motile aeromonad septicemia in cyprinid fish

Cited by 117 publications

References 55 publications

The zebrafish as a model for gastrointestinal tract–microbe interactions

The zebrafish as a model for gastrointestinal tract–microbe interactions

Aeromonas veronii Infection in Commercial Freshwater Fish: A Potential Threat to Public Health

Exploring the advantages and limitations of sampling methods commonly used in research facilities for zebrafish health inspections

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