<i>Aedes triseriatus</i>Females Transovarially Infected With La Crosse Virus Mate More Efficiently Than Uninfected Mosquitoes

Reese, Sara M.; Beaty, Meaghan K.; Gabitzsch, Elizabeth S.; Blair, Carol D.; Beaty, Barry J.

doi:10.1603/033.046.0524

Cited by 22 publications

(18 citation statements)

References 25 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Virus infection of insects by entomopathogenic and vectorborne viruses can cause both positive and negative effects on the development of the insect host (13,58). We found vitellogenin and actin (both within spot 374) to be upregulated in the TSWVexposed thrips.…”

Section: Discussionmentioning

confidence: 74%

Proteomic Analysis of Frankliniella occidentalis and Differentially Expressed Proteins in Response toTomato Spotted Wilt VirusInfection

Badillo-Vargas

Rotenberg

Schneweis

et al. 2012

J Virol

View full text Add to dashboard Cite

Tomato spotted wilt virus(TSWV) is transmitted byFrankliniella occidentalisin a persistent propagative manner. Despite the extensive replication of TSWV in midgut and salivary glands, there is little to no pathogenic effect onF. occidentalis. We hypothesize that the first-instar larva (L1) ofF. occidentalismounts a response to TSWV that protects it from pathogenic effects caused by virus infection and replication in various insect tissues. A partial thrips transcriptome was generated using 454-Titanium sequencing of cDNA generated fromF. occidentalisexposed to TSWV. Using these sequences, the L1 thrips proteome that resolved on a two-dimensional gel was characterized. Forty-seven percent of the resolved protein spots were identified using the thrips transcriptome. Real-time quantitative reverse transcriptase PCR (RT-PCR) analysis of virus titer in L1 thrips revealed a significant increase in the normalized abundance of TSWV nucleocapsid RNA from 2 to 21 h after a 3-h acquisition access period on virus-infected plant tissue, indicative of infection and accumulation of virus. We compared the proteomes of infected and noninfected L1s to identify proteins that display differential abundances in response to virus. Using four biological replicates, 26 spots containing 37 proteins were significantly altered in response to TSWV. Gene ontology assignments for 32 of these proteins revealed biological roles associated with the infection cycle of other plant- and animal-infecting viruses and antiviral defense responses. Our findings support the hypothesis that L1 thrips display a complex reaction to TSWV infection and provide new insights toward unraveling the molecular basis of this interaction.

show abstract

Section: Discussionmentioning

confidence: 74%

Proteomic Analysis of Frankliniella occidentalis and Differentially Expressed Proteins in Response toTomato Spotted Wilt VirusInfection

Badillo-Vargas

Rotenberg

Schneweis

et al. 2012

J Virol

View full text Add to dashboard Cite

show abstract

“…Interactions of Ae. triseriatus with other mosquitoes have been speculated to result in competitive displacement, spill‐over effects, and enhanced LACV vectorial capacity (Bevins 2007, 2008, Reese et al 2009, Leisnham and Juliano 2012, Westby et al 2016). Additionally, laboratory‐based interspecific competition studies demonstrated that Ae.…”

Section: Introductionmentioning

confidence: 99%

Aedes albopictus oviposits with other Aedes species in artificial oviposition cups: a case study in Knox County, Tennessee, U.S.A.

Dixson

Jackson

Rowe

et al. 2020

Journal of Vector Ecology

View full text Add to dashboard Cite

Interspecific associations between two mosquito species can lead to effects such as competition, species displacement, and species stability. To better understand Aedes albopictus (Skuse) and other Aedes species, we monitored eggs in artificial oviposition cups (ovitraps) within Knox County, TN, U.S.A., during the 2016 and 2017 mosquito seasons. In 2016, one black and one white ovitrap were placed at 18 sites for 21 weeks, while in 2017 black and white ovitraps baited with grass‐infused or deionized water were placed at 11 sites for nine weeks. Eggs were identified to species and resulting counts were used to determine the degree of interspecific association using Cole's coefficients (C7) and the degree of heterogeneity across space and time using generalized linear mixed models (GLMM). Here, Aedes mosquitoes are generally ovipositing in black cups with grass‐infused water, and Ae. albopictus eggs co‐occurred with other Aedes species more often than would be expected. Finding a positive significant interspecific association between Ae. albopictus and other Aedes eggs suggests that methods used to control Ae. albopictus may also control other Aedes mosquitoes. Finding that Ae. albopictus co‐occurs with other Aedes mosquitoes warrants additional research to evaluate outcomes associated with co‐occurrence within the study area.

show abstract

“…However, the rodent hosts do not exhibit overt illness (Borucki et al 2002). Furthermore, LACV can be transovarially transmitted from an infected female mosquito to her progeny, and recent studies have suggested that female mosquitoes infected with LACV mate more efficiently than uninfected mosquitoes (Reese et al 2009). Virus can be amplified substantially by this mechanism of transmission since each gonadotropic cycle leads to a multitude of infected progeny (Borucki et al 2002, Watts et al 1973).…”

Section: Bunyaviridaementioning

confidence: 99%

Arboviral Encephalitides: Transmission, Emergence, and Pathogenesis

Hollidge

González‐Scarano

Soldan

2010

J Neuroimmune Pharmacol

108

View full text Add to dashboard Cite

Arthropod-borne viruses (arboviruses) are of paramount concern as a group of pathogens at the forefront of emerging and re-emerging diseases. Although some arboviral infections are asymptomatic or present with a mild influenza-like illness, many are important human and veterinary pathogens causing serious illness ranging from rash and arthritis to encephalitis and hemorrhagic fever. Here, we discuss arboviruses from diverse families (Flaviviruses, Alphaviruses, and the Bunyaviridae) that are causative agents of encephalitis in humans. An understanding of the natural history of these infections as well as shared mechanisms of neuroinvasion and neurovirulence is critical to control the spread of these viruses and for the development of effective vaccines and treatment modalities.

show abstract

Aedes triseriatusFemales Transovarially Infected With La Crosse Virus Mate More Efficiently Than Uninfected Mosquitoes

Cited by 22 publications

References 25 publications

Proteomic Analysis of Frankliniella occidentalis and Differentially Expressed Proteins in Response toTomato Spotted Wilt VirusInfection

Proteomic Analysis of Frankliniella occidentalis and Differentially Expressed Proteins in Response toTomato Spotted Wilt VirusInfection

Aedes albopictus oviposits with other Aedes species in artificial oviposition cups: a case study in Knox County, Tennessee, U.S.A.

Arboviral Encephalitides: Transmission, Emergence, and Pathogenesis

Contact Info

Product

Resources

About