Adenanthera pavonina trypsin inhibitor retard growth of Anagasta kuehniella (Lepidoptera: Pyralidae)

Abstract

Anagasta kuehniella is a polyphagous pest that feeds on a wide variety of stored products. The possible roles suggested for seed proteinase inhibitors include the function as a part of the plant defensive system against pest via inhibition of their proteolytic enzymes. In this study, a trypsin inhibitor (ApTI) was purified from Adenanthera pavonina seed and was tested for insect growth regulatory effect. The chronic ingestion of ApTI did result in a significant reduction in larval survival and weight. Larval and pupal developmental time of larvae fed on ApTI diet at 1% was significantly longer; the larval period was extended by 5 days and pupal period was 10 days longer, therefore delaying by up to 20 days and resulting in a prolonged period of development from larva to adult. As a result, the ApTI diet emergence rate was only 28% while the emergence rate of control larvae was 80%. The percentage of surviving adults (%S) decreased to 62%. The fourth instar larvae reared on a diet containing 1% ApTI showed a decrease in tryptic activity of gut and that no novel proteolytic form resistant to ApTI was induced. In addition, the tryptic activity in ApTI -fed larvae was sensitive to ApTI. These results suggest that ApTI have a potential antimetabolic effect when ingested by A. kuehniella.

A Nonhost Peptidase Inhibitor of ~14 kDa fromButea monosperma(Lam.) Taub. Seeds Affects Negatively the Growth and Developmental Physiology ofHelicoverpa armigera

“…As the proportion of digested food metabolized for energy increases, ECD decreases [47, 48]. Therefore, it is suggested that the reduction in ECD is a likely outcome by undermining the efficiency to convert foodstuffs into growth, perhaps by a diversion of energy from production of biomass into detoxification of BmPI, that is, an increase in costs as reported elsewhere [43, 49]. …”

“…Table 4 shows that 0.10% w/w BmPI, incorporated into an artificial diet, decreases the growth, consumption rate, and fecal production of H. armigera larvae. These observations suggest that BmPI acts on intestinal tract and/or interferes with digestive capabilities of the insect or may be an outcome of physiological (postingestive) effects [43]. …”

The effects of a plant proteinase inhibitor from Enterolobium contortisiliquum on human tumor cell lines

“…Therefore, the search for effective inhibitors applicable in such clinical situations was and still is a challenging topic of tumor therapy-relevant research (Lah et al, 2006;Turk, 2006). In recent years, many proteinase inhibitors have been found in plants, especially in legume seeds Oliva and Sampaio, 2008;Roy and Dutta, 2009;Macedo et al, 2010;Prasad et al, 2010). One major aspect of interest in plant-derived inhibitors is to discover the relationship of structure and specificity or selectivity of these inhibitors for different proteinases enabling structural modifications to target a variety of proteinases, which are significantly involved in tumor development.…”

Trypsin inhibitors from Capsicum baccatum var. pendulum leaves involved in Pepper yellow mosaic virus resistance

“…Trypsin inhibitors are serine proteinase inhibitors, and high concentrations of such inhibitors are associated with plant resistance to insects, fungi, bacteria, and viruses (Kim et al, 2009, Macedo et al, 2010, Oliva et al, 2011. A successful strategy employing defense proteins in plant breeding requires the investigation of in vitro and in vivo activity against pathogens, determining the partial or full amino acid sequence, and purifying and characterizing the protein of interest (Oliveira and Macedo, 2011).…”