I.27 Plasma lactate levels increase during hperinsulinemic euglycemic clamp and oral glucose tolerance test

Berhane, Feven; Fite, Alemu; Daboul, Nour; Al-Janabi, Wissam; Msallaty, Zaher; Caruso, Martina; Lewis, Monique K.; Zhao, Yang; Diamond, M. P.; Abou‐Samra, Abdul Badi; Seyoum, Berhane

doi:10.1016/s0168-8227(14)70028-2

Cited by 2 publications

(1 citation statement)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…1,2 This is of particular importance in the case of clinical diagnostics, where an accurate diagnosis requires evidence from more than a single biomarker. 3 Apart from multi-analyte analysis, multiplexed systems are also used to endow LoCs with reliability through statistical analysis, 4 when requiring multiple control points 5,6 or when the reliability of the assay is subjected to sequential analysis, 7,8 among others.…”

Section: Introductionmentioning

confidence: 99%

Microfluidic-controlled optical router for lab on a chip

et al. 2019

View full text Add to dashboard Cite

show abstract

Section: Introductionmentioning

confidence: 99%

Microfluidic-controlled optical router for lab on a chip

et al. 2019

View full text Add to dashboard Cite

show abstract

Reconfigurable multiplexed point of Care System for monitoring type 1 diabetes patients

Márquez

Aymerich

Dei

et al. 2019

Biosensors and Bioelectronics

View full text Add to dashboard Cite

At the point of care (POC), on-side clinical testing allows fast biomarkers determination even in resource-limited environments. Current POC systems rely on tests selective to a single analyte or complex multiplexed systems with important portability and performance limitations. Hence, there is a need for handheld POC devices enabling the detection of multiple analytes with accuracy and simplicity. Here we present a reconfigurable smartphone-interfaced electrochemical Lab-on-a-Chip (LoC) with two working electrodes for dual analyte determination enabling biomarkers' selection in situ and on-demand. Biomarkers selection was achieved by the use of electrodepositable alginate hydrogels. Alginate membranes containing either glucose oxidase (GOx) or lactate oxidase (LOx) were selectively electrodeposited on the surface of each working electrode in around 4 min, completing sample measurement in less than 1 min. Glucose and lactate determination was performed simultaneously and without cross-talk in buffer, fetal bovine serum (FBS) and whole blood samples, the latter being possible by the sizeexclusion filtration capacity of the hydrogels. At optimal conditions, glucose and lactate were determined in a wide linear range (0 -12 mM and 0 -5 mM, respectively) and with high sensitivities (0.24 and 0.54 µA cm -2 mM -1 , respectively), which allowed monitoring of Type-1 diabetic patients with a simple dual analysis system. After the measurement, membranes were removed by disaggregation with the calcium-chelator phosphate buffer. At this point, new membranes could be electrodeposited, this time being selective to the same or another analyte. This conferred the system with on-demand biomarkers' selection capacity. The versatility and flexibility of the current architecture is expected to impact in POC analysis in applications ranging from homecare to sanitary emergencies.

show abstract

I.27 Plasma lactate levels increase during hperinsulinemic euglycemic clamp and oral glucose tolerance test

Cited by 2 publications

References 0 publications

Microfluidic-controlled optical router for lab on a chip

Microfluidic-controlled optical router for lab on a chip

Reconfigurable multiplexed point of Care System for monitoring type 1 diabetes patients

Contact Info

Product

Resources

About