110^th Anniversary: Fast and Easy-to-Use Method for Coating Tissue Culture Polystyrene Surfaces with Nonfouling Copolymers To Prevent Cell Adhesion

Abstract

Synthetic substrates able to prevent the adhesion of cells, commonly referred to as nonfouling surfaces, are useful in a wide range of applications, including the culture of multicellular spheroids and the engineering of complex cell sheets. Nonfouling properties are commonly conferred to surfaces through superhydrophilic polymer-based coatings. At the same time, this type of treatment requires difficult, expensive, and time-consuming procedures, leading to high prices of the final product. In this perspective, the development of quick single-step methods to coat surfaces with nonfouling polymers would dramatically reduce the costs and increase the flexibility of such products. Moreover, this strategy would enable the process to be carried out directly by the end user. In this work, we report a fast and easy-to-use coating method to prevent the nonspecific adhesion of cells on tissue culture polystyrene (TCPS) surfaces. Our approach is based on the adsorption of poly(styrene-co-3-sulfopropyl methacrylate) copolymers comprising nonfouling hydrophilic moieties as well as functionalities that enhance the polymer adhesion to the substrate. The polymer adsorption is obtained from an aqueous mixture with a procedure that can be conveniently performed in short time. The kinetic of polymer adsorption as well as the effect of the polymer concentration was studied in order to reduce the time and cost of the entire procedure. Additionally, the polymer composition and the polymer density were optimized to completely avoid the adhesion of three different adherent cell lines, that is, CHO, A375-P, and HFF-1 cells.

Polymer Nanoparticles for the Release of Fragrances: How the Physicochemical Properties Influence the Adsorption on Textile and the Delivery of Limonene

“…As it can be seen in Figure a, the equilibrium is reached within the first 2 h at all the concentrations tested. Such a long equilibration time was already reported in the literature for polymeric NPs and is in contrast with the faster adsorption kinetics of small molecules. ,, …”

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Readily Adsorbable Thermoresponsive Polymers for the Preparation of Smart Cell-Culturing Surfaces on Site

“…44 The thermoresponsive polymers Stat10, Block, and Block-RGD were adsorbed on TCPS surfaces from an alcoholic solution in 30 min at room temperature. By measuring the absorbance of the alcoholic solution, we estimated that at least 15% of the copolymer adsorbed on the TCPS (Figure S6, SI), in line with a previous report, 44 suggesting that the recovered solution could be conveniently recycled for the coating of other surfaces. It is important to notice that the adsorbed polymer is poorly prone to desorption, with a maximum percentage of copolymer detachment during the washing of the surface of only 2% (as shown in Figure S6, SI).…”

“…After the synthesis of the thermoresponsive polymers, we studied the possibility of obtaining a smart coating on TCPS surfaces with a simple protocol that could be directly applied in biological laboratories shortly before the seeding of the cells. 44 The thermoresponsive polymers Stat10, Block, and Block-RGD were adsorbed on TCPS surfaces from an alcoholic solution in 30 min at room temperature. By measuring the absorbance of the alcoholic solution, we estimated that at least 15% of the copolymer adsorbed on the TCPS (Figure S6, SI), in line with a previous report, 44 suggesting that the recovered solution could be conveniently recycled for the coating of other surfaces.…”

“…After the synthesis of the thermoresponsive polymers, we studied the possibility of obtaining a smart coating on TCPS surfaces with a simple protocol that could be directly applied in biological laboratories shortly before the seeding of the cells . The thermoresponsive polymers Stat10, Block, and Block-RGD were adsorbed on TCPS surfaces from an alcoholic solution in 30 min at room temperature.…”