hZIP1 that is down-regulated in clear cell renal cell carcinoma is negatively associated with the malignant potential of the tumor

Dong, Xiao; Kong, Chuize; Zhang, Zhe; Liu, Xiankui; Zhan, Bo; Chen, Zhipeng; Shi, Du

doi:10.1016/j.urolonc.2014.02.021

Cited by 9 publications

(10 citation statements)

References 28 publications

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“…This result suggested that HIF-1a tended to play different roles in normal renal or cancerous renal, it also played a role in physiological processes of normal renal and further verification is needed. Second, referring to our previous results, hZIP1 plays a vital role in the progression of renal cancer, knockdown of hZIP1 increases the proliferation and invasion of ACHN cells, and overexpression of hZIP1 diminishes the formation of tumors in nude mice by targeting GAS5/miR-223 (17,18). Moreover, zinc induced HIF-1a proteasomal degradation and suppressed VEGF expression in prostate cancer and glioblastoma (24).…”

Section: Discussionmentioning

confidence: 55%

“…Second, referring to our previous results, hZIP1 plays a vital role in the progression of renal cancer, knockdown of hZIP1 increases the proliferation and invasion of ACHN cells, and overexpression of hZIP1 diminishes the formation of tumors in nude mice by targeting GAS5/miR-223 ( 17 , 18 ). Moreover, zinc induced HIF-1α proteasomal degradation and suppressed VEGF expression in prostate cancer and glioblastoma ( 24 ).…”

Section: Discussionmentioning

confidence: 59%

“…The hZIP1 protein acted as a tumor suppressor in ccRCC according to our previous work ( 17 ). We believe that after the knockdown of hZIP1, the malignant progression of renal cancer could be expedited.…”

Section: Resultsmentioning

confidence: 99%

“…Moreover, the enhancement of HIF-1α-related glucose metabolism in tumor progression may be influenced by the expression reduction or fault of hZIP1. Our previous work showed that hZIP1 demonstrated lower expression in ccRCC tissues than in normal tissues, and after knockdown of hZIP1, the proliferation and invasion of renal cancer cells increased ( 17 ). This outcome illustrated that hZIP1 acted as a tumor suppressor in ccRCC.…”

Section: Introductionmentioning

confidence: 99%

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hZIP1 Inhibits Progression of Clear Cell Renal Cell Carcinoma by Suppressing NF-kB/HIF-1α Pathway

et al. 2021

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PurposeAccumulating literature has suggested that hZIP1 and HIF-1α play vital roles in the tumor process of clear cell renal cell carcinoma (ccRCC). However, the functional roles of hZIP1 and HIF-1α in ccRCC remain largely unknown.MethodsHIF-1α protein level was evaluated by a western blot in ccRCC tissues and cell lines. ccRCC cell lines were transfected with HIF-1α-siRNA to downregulate the expression level of HIF-1α. Then the proliferative, migratory and invasive abilities of ccRCC cells in vitro were detected by real-time cell analysis (RTCA) assay, wound healing assay and transwell assay, respectively. The role of HIF-1α in vivo was explored by tumor implantation in nude mice. Then the effect on glycolysis‐related proteins was performed by western blot after hZIP1 knockdown (overexpression) or HIF-1α knockdown. The effect on NF‐kB pathway was detected after hZIP1 overexpression.ResultsHIF-1α was markedly downregulated in ccRCC tissues compared with normal areas. But HIF-1α presented almost no expression in HK-2 and ACHN cells. Immunofluorescence indicated HIF-1α and PDK1 expression in both the cytoplasm and nucleus in ccRCC cells. Downregulation of HIF-1α suppressed ccRCC cell proliferation, migration, and invasion and resulted in smaller implanted tumors in nude mice. Furthermore, hZIP1 knockdown elevated HIF-1α protein levels and PDK1 protein levels in ccRCC cells. Interestingly, a sharp downregulated expression of HIF-1α was observed after hZIP1 overexpression in OSRC-2 and 786-O cells, which resulted from a downtrend of NF-kB1 moving into the cell nucleus.ConclusionOur work has vital implications that hZIP1 suppresses ccRCC progression by inhibiting NF-kB/HIF-1α pathway.

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Section: Discussionmentioning

confidence: 55%

Section: Discussionmentioning

confidence: 59%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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hZIP1 Inhibits Progression of Clear Cell Renal Cell Carcinoma by Suppressing NF-kB/HIF-1α Pathway

et al. 2021

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“…However ACHN, among the most commonly referenced RCC cell line, clusters with a subset of papillary RCC. Although ACHN has been previously recognized to be of papillary origin based on chromosomal alteration patterns in 7 and 17 (refs 38, 39), several studies continue to utilize it as a standard model for RCC404142. Similarly, U031 and CAL54, the other two cell lines that cluster with pRCC, also exhibit gains on chromosomes 7, 16 and 17, like ACHN.…”

Section: Discussionmentioning

confidence: 99%

Analysis of renal cancer cell lines from two major resources enables genomics-guided cell line selection

et al. 2017

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The utility of cancer cell lines is affected by the similarity to endogenous tumour cells. Here we compare genomic data from 65 kidney-derived cell lines from the Cancer Cell Line Encyclopedia and the COSMIC Cell Lines Project to three renal cancer subtypes from The Cancer Genome Atlas: clear cell renal cell carcinoma (ccRCC, also known as kidney renal clear cell carcinoma), papillary (pRCC, also known as kidney papillary) and chromophobe (chRCC, also known as kidney chromophobe) renal cell carcinoma. Clustering copy number alterations shows that most cell lines resemble ccRCC, a few (including some often used as models of ccRCC) resemble pRCC, and none resemble chRCC. Human ccRCC tumours clustering with cell lines display clinical and genomic features of more aggressive disease, suggesting that cell lines best represent aggressive tumours. We stratify mutations and copy number alterations for important kidney cancer genes by the consistency between databases, and classify cell lines into established gene expression-based indolent and aggressive subtypes. Our results could aid investigators in analysing appropriate renal cancer cell lines.

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Upregulated Solute Carrier SLC39A1 Promotes Gastric Cancer Proliferation and Indicates Unfavorable Prognosis

Chen

Luo

et al. 2022

Genetics Research

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Backgrounds. Solute carrier 39A1 (SLC39A1) is an indirect zinc transporter which showed diverse tumor-related functions in different malignancies. Here, we aimed to investigate its expression and role in gastric adenocarcinoma. Methods. A retrospective gastric adenocarcinoma cohort (n = 154) was collected from our hospital to test their tissue expression of SLC39A1 through immunohistochemical staining method. After SLC39A1 overexpression or knockdown, proliferation and invasion assays were conducted for proliferation and invasion estimation, respectively. Xenograft in nude mice was used as the in vivo strategy to validate in vitro findings. Results. Compared with adjacent stomach tissues, gastric adenocarcinoma tissues showed significantly higher SLC39A1 on both mRNA and protein levels. Higher SLC39A1 was observed in patients with larger tumor size ( P = 0.003 ) and advanced tumor stages ( P < 0.001 ). Univariate ( P = 0.001 ) and multivariate analyses ( P = 0.035 ) confirmed the independent prognostic significance of SLC39A1 on gastric adenocarcinoma outcomes. The median survival time was 22.0 months in patients with high-SLC39A1 expression, while up to 57.0 months in those with low-SLC39A1 ( P = 0.001 ). In vitro and in vivo assays demonstrated that overexpressing SLC39A1 could promote gastric cancer growth and invasion, while silencing SLC39A1 led to opposite effects. Conclusions. Aberrant high-SLC39A1 expression can serve as an independent unfavorable prognostic factor for gastric adenocarcinoma. High SLC39A1 is critical for a more aggressive tumor phenotype by promoting cell proliferation and invasion. Therefore, targeting SLC39A1 may provide novel therapeutic insights.

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hZIP1 that is down-regulated in clear cell renal cell carcinoma is negatively associated with the malignant potential of the tumor

Cited by 9 publications

References 28 publications

hZIP1 Inhibits Progression of Clear Cell Renal Cell Carcinoma by Suppressing NF-kB/HIF-1α Pathway

hZIP1 Inhibits Progression of Clear Cell Renal Cell Carcinoma by Suppressing NF-kB/HIF-1α Pathway

Analysis of renal cancer cell lines from two major resources enables genomics-guided cell line selection

Upregulated Solute Carrier SLC39A1 Promotes Gastric Cancer Proliferation and Indicates Unfavorable Prognosis

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