“…The compounds displayed a diverse array of bioactivities such as antimycobacterial, cytotoxicity, anti-PLA2, and antimicrobial activity Youssef et al,2002;Youssef, 2005;Sauleau et al,2006;Youssef et al,2004). These results encouraged us to look at the symbiotic fungal biodiversity in the Red Sea sponges Hyrtios erecta Youssef et al,2002) and Hyrtios erectus (Youssef, 2005;Sauleau et al,2006) and evaluate the antimicrobial activities of these fungi as a potential and future source of drug leads. Herein we mixture for PCR amplification contained 5 μL of 10 × reaction buffer with 15 mM MgCl2 (Invitrogen), 2 μL of 2.5 mM dNTPs, 0.5 μL of 10 µM each primer, 4 μL of fungal DNA, 0.3 μL of Taq DNA polymerase (5 U·μL−1, Invitrogen), and 39.7 μL of H 2 O. PCR conditions included an initial denaturation at 94 ºC for 4 min followed by 30 cycles of denaturation at 94 ºC for 50 s, annealing at 51 ºC for 50 s, and elongation at 68 °C for 1 min, with a final elongation at 68 ºC for 10 min.…”