Hyriopsis cumingii Hic52—A novel nacreous layer matrix protein with a collagen-like structure

Liu, Xiao Jun; Pu, Jingwen; Zeng, Shimei; Jin, Can; Dong, Shaojian; Li, Jiale

doi:10.1016/j.ijbiomac.2017.03.154

Cited by 12 publications

(5 citation statements)

References 26 publications

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“…Hic52 is a very basic (theoretical pI > 10), Gly- and Gln-rich protein, with few poly-Q and poly-G blocks and several degenerate G-rich repeats of different lengths along the sequence. It possesses a collagen-like structure which suggests a structural role in nacre formation (Liu et al, 2017b). Silkmapin (GenBank: AIZ03589.1, and its isoforms nasilin 1 and 2) are Gly-rich non-acidic proteins with a structural function, probably related to the formation of both nacreous and prismatic layers (Liu et al, 2015; Marie et al, 2017).…”

Section: Resultsmentioning

confidence: 99%

“…It has poly-glutamine and poly-glycine blocks with several degenerate repeats of different lengths along the sequence. It was reported to have collagen-like structure (Liu et al, 2017b) and probably plays a structural role in nacre formation.Silkmapin (GenBank: AIZ03589.1) and its isoforms (nasilin 1 and 2 (GenBank: ASQ40996.1 and ASQ40997.1)) are glycine-rich (>34%), non-acidic proteins with a structural function and probably play a role in the deposition of both nacre and prismatic layers (Liu et al, 2015). Chitin deacetylase enzyme (present in isoforms A and B (GenBank: AFO53262.1 and AFO53263.1)) is a slightly basic (IP 8.34) protein, with a chitin deacetylation function and possibly has a role in the 3D structuring of the mineral framework.Carbonic anhydrase 3 ( Hyriopsis cumingii , GenBank: ARG42317.1) is an asparagine (10.2%) and threonine (9.3%) rich, acidic (IP 5.9) protein.…”

Section: 1 Archaeological Sites and Double-buttonsmentioning

confidence: 99%

Section: 1 Archaeological Sites and Double-buttonsmentioning

confidence: 99%

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'Palaeoshellomics’ reveals the use of freshwater mother-of-pearl in prehistory

Sakalauskaite

Andersen

Biagi

et al. 2019

eLife

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The extensive use of mollusc shell as a versatile raw material is testament to its importance in prehistoric times. The consistent choice of certain species for different purposes, including the making of ornaments, is a direct representation of how humans viewed and exploited their environment. The necessary taxonomic information, however, is often impossible to obtain from objects that are small, heavily worked or degraded. Here we propose a novel biogeochemical approach to track the biological origin of prehistoric mollusc shell. We conducted an in-depth study of archaeological ornaments using microstructural, geochemical and biomolecular analyses, including ‘palaeoshellomics’, the first application of palaeoproteomics to mollusc shells (and indeed to any invertebrate calcified tissue). We reveal the consistent use of locally-sourced freshwater mother-of-pearl for the standardized manufacture of ‘double-buttons’. This craft is found throughout Europe between 4200–3800 BCE, highlighting the ornament-makers’ profound knowledge of the biogeosphere and the existence of cross-cultural traditions.

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Section: Resultsmentioning

confidence: 99%

Section: 1 Archaeological Sites and Double-buttonsmentioning

confidence: 99%

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'Palaeoshellomics’ reveals the use of freshwater mother-of-pearl in prehistory

Sakalauskaite

Andersen

Biagi

et al. 2019

eLife

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“…To date, several matrix proteins, including 48 kDa glycosylation protein , hcperlucin , HcCA , silkmapin , hic22 , hic24 , hic31 , hic52 , hic74 , HcTyr , HcTyp‐1 , pif , and Hc‐upsalin , have been isolated and identified from the shell of H. cumingii . These matrix proteins play an important role in controlling the CaCO 3 sedimentation speed, regulating the crystalline form, and regulating nacre deposition during pearl mineralization .…”

Section: Introductionmentioning

confidence: 99%

Identification of nacre matrix protein genes hic14 and hic19 and their roles in crystal growth and pearl formation in the mussel Hyriopsis cumingii

Jin¹,

Ren

Pu³

et al. 2019

Biotech and App Biochem

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Biological mineralization is a highly programmed process in which inorganic minerals reassociate under the strict control of the extracellular matrix to form minerals with special functions and patterns. Shells are biominerals, and their synthesis is finely regulated by organic matrix including matrix proteins, polysaccharides, lipids, pigments, free amino acids, and small peptides. In this study, two matrix protein genes, hic14 and hic19, were isolated from the mantle of the mussel Hyriopsis cumingii. Tissue expression analysis showed that both proteins were expressed mainly in the mantle, and in situ hybridization of mantle tissues showed that they were specifically expressed in the dorsal epithelial cells of mantle pallial. Therefore, hic14 and hic19 were both nacreous layer matrix proteins. In the pearl insertion experiment, hic14 and hic19 kept low expression during pearl sac formation and disordered calcium carbonate deposition, and increased significantly during pearl nacre accumulation, which showed that both proteins participated in the mineralization of pearl nacre. In the RNA interference experiment, shell nacre tablet growth was inhibited after crystal nucleation due to the decreased expression of hic14, and crystal morphology and arrangement of nacre were highly modified after expression of hic19 was inhibited. These results provided further evidence that both hic14 and hic19 participated in nacreous layer biomineralization.

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“…Most of these proteins share specific domains which have been related to functions such as inhibitors of growth and precipitation of calcite 15 and/or inducers of aragonite nucleation 4 . Proteins such as MSI60 acts as structural support for crystal nucleation and growth due to the self-assembly of the protein forming a fiber-like structure 9 , other structural proteins includes hic31 and hic52, both containing polyglycine blocks of (Gly) n and structure similar to collagen type I, alpha 1 and alpha 2 [16][17][18] . Pearlin is an insoluble SMPs that has drawn attention since is involved in the shell nacre formation and the pearl of oysters.…”

mentioning

confidence: 99%

A shell matrix protein of Pinctada mazatlanica produces nacre platelets in vitro

Rivera-Pérez

Flores-Sánchez

Areyano

et al. 2020

Sci Rep

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Nacre is the main component of the pearl oyster shells and it is synthesized by specialized soluble and insoluble shell matrix proteins. Insoluble proteins from the decalcification of the shell are the less studied proteins due to the technical problems to isolate them from the organic matrix. In this study, an insoluble shell matrix protein from Pinctada mazatlanica, pearlin (Pmaz-pearlin), was successfully cloned from the mantle tissue, and the native protein isolated from the shell was functionally characterized. The full coding sequence of Pmaz-pearlin mRNA consists of 423 base pairs, which encode to a 16.3 kDa pearlin. Analysis of the deduced amino acid sequence revealed that Pmaz-pearlin contained four acidic regions, an NG repeat domain, and Cys conserved residues, the latter potentially forms four disulfide bridges which might stabilize the protein structure. The isolated protein from the shell is a glycoprotein of ~ 16.74 kDa which can produce aragonite and calcite crystals in vitro. Our results show that Pmaz-pearlin is a well-conserved protein involved in nacre layer growth, which produces calcite crystals in the presence of CaCl2, aragonite crystal polymorphs with a hexagonal structure in the presence of MgCl2, and needle-like crystal structure polymorphs in the presence of CaCO3 The identity of the crystals was confirmed using RAMAN analyses.

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Hyriopsis cumingii Hic52—A novel nacreous layer matrix protein with a collagen-like structure

Cited by 12 publications

References 26 publications

'Palaeoshellomics’ reveals the use of freshwater mother-of-pearl in prehistory

'Palaeoshellomics’ reveals the use of freshwater mother-of-pearl in prehistory

Identification of nacre matrix protein genes hic14 and hic19 and their roles in crystal growth and pearl formation in the mussel Hyriopsis cumingii

A shell matrix protein of Pinctada mazatlanica produces nacre platelets in vitro

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